Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass
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Steam-exploded corn stover biomass was used as the substrate for fed-batch separate enzymatic hydrolysis and fermentation (SHF) to investigate the solid concentration ranging from 10% to 30% (w/w) on the lignocellulose enzymatic hydrolysis and fermentation. The treatment of washing the steam-exploded material was also evaluated by experiments. The results showed that cellulose conversion changed little with increasing solid concentration, and fermentation by Saccharomyces cerevisiae revealed a nearly same ethanol yield with the water-washed steam-exploded corn stover. For the washed material at 30% substrate concentration, i.e., 30% water insoluble solids (WIS), enzymatic hydrolysis yielded 103.3 g/l glucose solution and a cellulose conversion of 72.5%, thus a high ethanol level up to 49.5 g/l. With the unwashed steam-exploded corn stover, though a cellulose conversion of 70.9% was obtained in hydrolysis at 30% solid concentration (27.9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production.
KeywordsBioethanol Corn stover High solid concentration Enzymatic hydrolysis Fermentation
- 11.Kalman, G., Varga, E., & Reczey, K. (2002). Diluted sulphuric acid pretreatment of corn stover at long residence times. Chem Biochem Eng Q, 16, 151–157.Google Scholar
- 13.Schell, D. (2005). 2005 OBP biennial peer review, energy efficiency and renewable energy. http://programreview.biomass.govtools.us/%5Cdocuments%5C11e80e62-6e8a-4858-a0e5-1fa75a591116.ppt
- 24.Palmqvist, E., Grage, H., Meinander, N. Q., & Hahn-Hagerdal, B. (1999). Main and interaction effects of acetic acid, furfural, and p-hydroxybenzoic acid on growth and ethanol productivity of yeasts. Biotechnology and Bioengineering, 63, 46–55. doi:10.1002/(SICI)1097-0290(19990405)63:1<46::AID-BIT5>3.0.CO;2-J.CrossRefGoogle Scholar
- 25.National Renewable Energy Laboratory (NREL). Chemical analysis and testing laboratory analytical procedures, LAP-002 (1996), LAP-003 (1995), LAP-004 (1996), LAP-005 (1994), LAP-010 (1994) and LAP-017 (1998), NREL, Golden, CO, USA. http://www.eere.energy.gov/biomass/analytical_procedures.html.
- 26.Öhgren, K., Bengtsson, O., Gorwa, M. F., Galbe, M., Hahn, B., & Zacchi, G. (2006). Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400. Journal of Biotechnology, 126, 488–498. doi:10.1016/j.jbiotec.2006.05.001.CrossRefGoogle Scholar