Applied Biochemistry and Biotechnology

, Volume 160, Issue 2, pp 360–369 | Cite as

Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass

  • Yifeng Lu
  • Yonghong Wang
  • Guoqian Xu
  • Ju Chu
  • Yingping Zhuang
  • Siliang Zhang


Steam-exploded corn stover biomass was used as the substrate for fed-batch separate enzymatic hydrolysis and fermentation (SHF) to investigate the solid concentration ranging from 10% to 30% (w/w) on the lignocellulose enzymatic hydrolysis and fermentation. The treatment of washing the steam-exploded material was also evaluated by experiments. The results showed that cellulose conversion changed little with increasing solid concentration, and fermentation by Saccharomyces cerevisiae revealed a nearly same ethanol yield with the water-washed steam-exploded corn stover. For the washed material at 30% substrate concentration, i.e., 30% water insoluble solids (WIS), enzymatic hydrolysis yielded 103.3 g/l glucose solution and a cellulose conversion of 72.5%, thus a high ethanol level up to 49.5 g/l. With the unwashed steam-exploded corn stover, though a cellulose conversion of 70.9% was obtained in hydrolysis at 30% solid concentration (27.9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production.


Bioethanol Corn stover High solid concentration Enzymatic hydrolysis Fermentation 


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Copyright information

© Humana Press 2008

Authors and Affiliations

  • Yifeng Lu
    • 1
  • Yonghong Wang
    • 1
  • Guoqian Xu
    • 1
  • Ju Chu
    • 1
  • Yingping Zhuang
    • 1
  • Siliang Zhang
    • 1
  1. 1.State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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