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Kinetic Analysis and pH-Shift Control Strategy for Propionic Acid Production with Propionibacterium Freudenreichii CCTCC M207015

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Abstract

The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a 7.5-l stirred-tank fermentor. Batch fermentations by P. freudenreichii CCTCC M207015 at various pH values ranging from 5.5 to 7.0 were studied. Based on the analysis of the time course of specific cell growth rate (μ x) and specific propionic acid formation rate (μ p), a two-stage pH-shift control strategy was proposed. At first 48 h, pH was controlled at 6.5 to obtain the maximal μ x, subsequently pH 6.0 was used to maintain high μ p to enhance the production of propionic acid. By applying this pH-shift control strategy in propionic acid fermentation, the maximal propionic acid and glucose conversion efficiency had a significant improvement and reached 19.21 g/l and 48.03%, respectively, compared with those of constant pH operation (14.58 g/l and 36.45%). Fed-batch fermentation with pH-shift control strategy was also applied to produce propionic acid; the maximal propionic acid yield and glucose conversion efficiency reached 25.23 g/l and 47.76%, respectively.

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Acknowledgements

This work was supported by the National Basic Research Program of China (973) (2007CB714304), the National High Technology Research and Development Program of China (2006AA03Z453), and the National Nature Science Foundation of China (20674038).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 210009, People’s Republic of China

    Xiaohai Feng, Hong Xu, Jun Yao, Sha Li, Hongyang Zhu & Pingkai Ouyang

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  1. Xiaohai Feng
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  2. Hong Xu
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  3. Jun Yao
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  4. Sha Li
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Correspondence to Hong Xu.

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Feng, X., Xu, H., Yao, J. et al. Kinetic Analysis and pH-Shift Control Strategy for Propionic Acid Production with Propionibacterium Freudenreichii CCTCC M207015. Appl Biochem Biotechnol 160, 343–349 (2010). https://doi.org/10.1007/s12010-008-8300-6

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  • DOI: https://doi.org/10.1007/s12010-008-8300-6

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