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Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.)

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Abstract

Two peroxidases, cPOD-I and rPOD-II, have been isolated and purified from cotton cell suspension and their biochemical characteristics studied. rPOD-II from R405-2000, a non-embryogenic cultivar, has higher activity than cPOD-I derived from Coker 312, which developed an embryogenic structure. The cPOD-I and rPOD-II had molecular mass of 39.1 and 64 kDa respectively, as determined by SDS-PAGE. Both enzymes showed high efficiency of interaction with the guaiacol at 25 mM. The optimal pH for cPOD-I and rPOD-II activity was 5.0 and 6.0, respectively. The enzyme had an optimum temperature of 25 °C and was relatively stable at 20–30 °C. The isoenzymes were highly inhibited by ascorbic acid, dithiothreitol, sodium metabisulfite, and β-mercaptoethanol. Their activities were highly enhanced by Al3+, Fe3+, Ca2+, and Ni2+, but they were moderately inhibited by Mn2+ and K+. The enzyme lost 50% to 62% of its activity in the presence of Zn2+ and Hg2+.

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Acknowledgments

The authors are grateful to CIRAD and CNRA for supplying cottonseeds. We thank Dr. Bognonkpe J.P. for its contribution.

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  1. Laboratoire de Biologie et d’Amélioration des Productions Végétales, UFR Sciences de la Nature, Université d’Abobo-Adjamé, 02 BP 801, Abidjan 02, Ivory Coast

    Tanoh Hilaire Kouakou & Yatty Justin Kouadio

  2. Laboratoire de Biocatalyse, UFR des Sciences et technologie des Aliments, Université d’Abobo-Adjamé, 02 BP 801, Abidjan 02, Ivory Coast

    Edmond Ahipo Dué & N’guessan Eugène Jean Parfait Kouadio

  3. Laboratoire de Biotechnologies, UFR Biosciences, Université de Cocody, 22 BP 582, Abidjan 02, Ivory Coast

    Sébastien Niamké

  4. Groupe d’Etude des Substances Végétales à Activités Biologiques, UFR des Sciences Pharmaceutiques, Université de Bordeaux 2, 146 rue Léo-Saignat, 33076, Bordeaux cedex, France

    Jean-Michel Mérillon

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  1. Tanoh Hilaire Kouakou
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Kouakou, T.H., Dué, E.A., Kouadio, N.E.J.P. et al. Purification and Characterization of Cell Suspensions Peroxidase from Cotton (Gossypium hirsutum L.). Appl Biochem Biotechnol 157, 575–592 (2009). https://doi.org/10.1007/s12010-008-8287-z

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