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Organic Phase Synthesis of Ethyl Oleate Using Lipases Produced by Solid-state Fermentation

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Abstract

This paper reports a study of the enzymatic esterification of oleic acid and ethanol. The reaction was catalyzed by lipases produced by solid-state fermentation with Rhizopus sp. Olive oil and perlite were used as an inducer and inert support, respectively. Synthesis of ethyl oleate was carried out in a 10-mL batch reactor with magnetic stirring. The effects of substrate ratios, biocatalyst concentration, and temperature on the reaction rate and conversion efficiency were evaluated. The highest reaction rate (1.64 mmol/L min) was reached with an oleic acid/ethanol mol ratio of 1:5 (oleic acid 50 mM:ethanol 250 mM) and 1 g of biocatalyst. Conversions approaching 100% were obtained after 60 min of reaction at 45 °C with n-hexane as a solvent. The initial reaction rate increased proportionally with respect to biocatalyst concentration, which suggests that the reaction rate was not controlled by mass transfer. The biocatalyst retained more than 80% of its catalytic activity after 7 months of storage at 4 °C. The results demonstrate that the biocatalyst produced by Rhizopus sp. in solid-state fermentation can be successfully used for ethyl oleate synthesis over short reaction periods under conditions when ethanol is in excess.

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Acknowledgments

This work was supported by the Mexican Council for Science and Technology (CONACyT). The authors gratefully thank Dr. Jesus A. Cordova Lopez for the Rhizopus strain used in this work.

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Authors and Affiliations

  1. Departamento de Biotecnología, Universidad Autónoma Metropolitana—Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Mexico City, C.P., 09340, Mexico

    Antonio Martínez-Ruiz, Gerardo Saucedo-Castañeda & Ernesto Favela-Torres

  2. Instituto Tecnológico de Veracruz, Calzada Miguel Ángel de Quevedo 2779, Col. Formando Hogar, Veracruz, C.P., 91897, Mexico

    Hugo Sergio García

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  1. Antonio Martínez-Ruiz
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  2. Hugo Sergio García
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  3. Gerardo Saucedo-Castañeda
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  4. Ernesto Favela-Torres
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Correspondence to Ernesto Favela-Torres.

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Martínez-Ruiz, A., García, H.S., Saucedo-Castañeda, G. et al. Organic Phase Synthesis of Ethyl Oleate Using Lipases Produced by Solid-state Fermentation. Appl Biochem Biotechnol 151, 393–401 (2008). https://doi.org/10.1007/s12010-008-8207-2

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  • DOI: https://doi.org/10.1007/s12010-008-8207-2

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