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Processing Parameters Matching Effects upon Rhizobium tropici Biopolymers’ Rheological Properties

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Abstract

The combined effects of the processing parameters upon rheological properties of biopolymers produced by Rhizobium tropici were studied as a function of the Ca+2 ions’ concentration variation, yeast extract concentration added to the medium, aeration, and agitation, maintaining the mannitol concentration in 10 g/L. The experiments were carried out using a fermenter with 20-L capacity as a reactor. All processing parameters were monitored online. The temperature [(30 ± 1) °C] and pH values (7.0) were kept constant throughout the experimental time. As a statistical tool, a complete 23 factorial design with central point and response surface was used to investigate the interactions between relevant variables of the fermentation process: calcium carbonate concentration, yeast extract concentration, aeration, and agitation. The processing parameter setup for reaching the maximum response for rheological propriety production was obtained when applying mannitol concentration of 10.0 g/L, calcium carbonate concentration 1.0 g/L, yeast extract concentration 1.0 g/L, aeration 1.30 vvm, and agitation 800 rpm. The viscosimetric investigation of polysaccharide solutions exposed their shear-thinning behavior and polyelectrolytic feature.

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Acknowledgements

The authors would like to thank National Council for Scientific and Technological Development and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Foundation (ICCTI 049/00) for financial support to conduct this work.

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Authors and Affiliations

  1. Chemical and Textile Industry Technological Center, Textile Industrial Engineering, Rio de Janeiro, Brazil

    Flávia Duta Pimenta

  2. Professor Eloisa Mano Macromolecules Institute, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

    Léa Maria de Almeida Lopes

  3. Department of Biochemistry Engeneering, Rio de Janeiro Federal University, Rio de Janeiro, Brazil

    Francisca Pessôa de França

Authors
  1. Flávia Duta Pimenta
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  2. Léa Maria de Almeida Lopes
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  3. Francisca Pessôa de França
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Correspondence to Flávia Duta Pimenta or Francisca Pessôa de França.

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Pimenta, F.D., Lopes, L.M.d.A. & de França, F.P. Processing Parameters Matching Effects upon Rhizobium tropici Biopolymers’ Rheological Properties. Appl Biochem Biotechnol 150, 33–49 (2008). https://doi.org/10.1007/s12010-008-8202-7

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