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New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation

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Abstract

Studying biofilm formation and influence of the matrix composition was heavy because only old and long methods were employed up to now: confocal microscopy, fluorescent chemical markers, and/or dying techniques. In this context, an innovative tool, the BioFilm Ring Test®, was here employed to explore the role of exopolysaccharides, proteins, and nucleic acids in the formation of biofilm by Leuconostoc mesenteroides. The principle is to add magnetic particles in the culture medium. When a biofilm is formed, particles are unable to migrate in the media to form a ring when a magnet is brought nearer to the well. Therefore, culture media supplemented with proteases, glycanases, and/or nucleases allowed us to identify the involvement of these substances in L. mesenteroides biofilm formation. The results permitted to demonstrate that dextran, proteins, and nucleic acids are implied in biofilm formation.

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Authors and Affiliations

  1. BioFilm Control, 27 rue Jean Claret, la Pardieu, 63000, Clermont Ferrand, France

    S. Badel & T. Bernardi

  2. Laboratoire de Génie Chimique et Biochimique, Polytech’ Clermont Ferrand, Université Blaise Pascal, 24 avenue des Landais, P.O. Box 206, 63174, Aubière cedex, France

    S. Badel, C. Laroche, C. Gardarin & P. Michaud

Authors
  1. S. Badel
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  2. C. Laroche
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  3. C. Gardarin
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  4. T. Bernardi
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  5. P. Michaud
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Correspondence to P. Michaud.

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Badel, S., Laroche, C., Gardarin, C. et al. New Method Showing the Influence of Matrix Components in Leuconostoc mesenteroides Biofilm Formation. Appl Biochem Biotechnol 151, 364–370 (2008). https://doi.org/10.1007/s12010-008-8199-y

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  • DOI: https://doi.org/10.1007/s12010-008-8199-y

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