Abstract
Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in different carriers were carried out for six cycles at optimal conditions. It was found that 33%, 38%, and 47% of activity was lost with chitosan, gelatin, and agar, respectively as immobilizing supports after the sixth cycle of operation.
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Nigam, V.K., Kundu, S. & Ghosh, P. Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid. Appl Biochem Biotechnol 141, 119–125 (2007). https://doi.org/10.1007/s12010-007-9214-4
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DOI: https://doi.org/10.1007/s12010-007-9214-4