Skip to main content

Enzymatic Hydrolysis and Ethanol Fermentation of High Dry Matter Wet-Exploded Wheat Straw at Low Enzyme Loading

Applied Biochemistry and Biotechnology volume 148, pages 35–44 (2008)Cite this article

Abstract

Wheat straw was pretreated by wet explosion using three different oxidizing agents (H2O2, O2, and air). The effect of the pretreatment was evaluated based on glucose and xylose liberated during enzymatic hydrolysis. The results showed that pretreatment with the use of O2 as oxidizing agent was the most efficient in enhancing overall convertibility of the raw material to sugars and minimizing generation of furfural as a by-product. For scale-up of the process, high dry matter (DM) concentrations of 15–20% will be necessary. However, high DM hydrolysis and fermentation are limited by high viscosity of the material, higher inhibition of the enzymes, and fermenting microorganism. The wet-explosion pretreatment method enabled relatively high yields from both enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) to be obtained when performed on unwashed slurry with 14% DM and a low enzyme loading of 10 FPU/g cellulose in an industrial acceptable time frame of 96 h. Cellulose and hemicellulose conversion from enzymatic hydrolysis were 70 and 68%, respectively, and an overall ethanol yield from SSF was 68%.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Kim, S., & Dale, B. E. (2004). Biomass Bioenergy, 26, 361–375.

    Article  Google Scholar 

  2. Jørgensen, H., Kristensen, J., & Felby, C. (2007). Biofuels Bioproducts and Biorefinery, 1, 119–134.

    Article  CAS  Google Scholar 

  3. Schmidt, A. S., & Thomsen, A. B. (1998). Bioresource Technology, 64, 139–151.

    Article  CAS  Google Scholar 

  4. Ballesteros, I., Negro, M. J., Oliva, J. M., Cabanas, A., & Ballesteros, M. (2006). Applied Biochemistry and Biotechnology, 129–132, 496–508.

    Article  Google Scholar 

  5. Saha, B. C., Iten, L. B., Cotta, M. A., & Wu, Y. V. (2005). Process Biochemistry, 40, 3693–3700.

    Article  CAS  Google Scholar 

  6. Thomsen, M. H., Thygesen, A., Jørgensen, H., Larsen, J., Christensen, B. H., & Thomsen, A. B. (2006). Applied Biochemistry and Biotechnology, 129–132, 448–460.

    Google Scholar 

  7. Ahring, B. K. & Munck, J. (2006) Patent no. WO 2006-032282 A1.

  8. Mohagheghi, A., Tucker, M., Grohmann, K., & Wyman, C. (1992). Applied Biochemistry and Biotechnology, 33, 67–81.

    CAS  Article  Google Scholar 

  9. Jørgensen, H., Vibe-Pedersen, J., Larsen, J., & Felby, C. (2007). Biotechnology and Bioengineering, 96, 862–870.

    Article  CAS  Google Scholar 

  10. Tengborg, C., Galbe, M., & Zacchi, G. (2001). Enzyme and Microbial Technology, 28, 835–844.

    Article  CAS  Google Scholar 

  11. Felby, C., Klinke, H. B., Olsen, H. S., & Thomsen, A. B. (2003). Applications of Enzymes to Lignocellulosics, 855, 157–174.

    CAS  Google Scholar 

  12. Galbe, M., & Zacchi, G. (2002). Applied Microbiology Biotechnology, 59, 618–628.

    Article  CAS  Google Scholar 

  13. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluite, J., Templeton, D., & Crocker, D. (2006). http://devafdc.nrel.gov/pdfs/9572.pdf.

  14. Hartmann, H., & Ahring, B. K. (2005). Water Research, 39, 1543–1552.

    Article  CAS  Google Scholar 

  15. American Public Health Association/American Water Works Association/Water Environmental Federation (1995) Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association/American Water Works Association/Water Environmental Federation, Washington DC, USA.

  16. Georgieva, T. I., Mikkelsen, M. J., & Ahring, B. K. (2007). Applied Biochemistry and Biotechnology DOI 10.1007/s12010-007-8014-1.

  17. Lu, Y. P., Yang, B., Gregg, D., Saddler, J. N., & Mansfield, S. D. (2002). Applied Biochemistry and Biotechnology, 98, 641–654.

    Article  Google Scholar 

  18. Tengborg, C., Galbe, M., & Zacchi, G. (2001). Biotechnology Progress, 17, 110–117.

    Article  CAS  Google Scholar 

  19. Ingesson, H., Zacchi, G., Yang, B., Esteghlalian, A. R., & Saddler, J. N. (2001). Journal of Biotechnology, 88, 177–182.

    Article  CAS  Google Scholar 

  20. Xiao, Z. Z., Zhang, X., Gregg, D. J., & Saddler, J. N. (2004). Applied Biochemistry and Biotechnology, 113–16, 1115–1126.

    Article  Google Scholar 

  21. Panagiotou, G., & Olsson, L. (2007). Biotechnology and Bioengineering, 96, 250–258.

    Article  CAS  Google Scholar 

  22. Varga, E., Klinke, H. B., Reczey, K., & Thomsen, A. B. (2004). Biotechnology and Bioengineering, 88, 567–574.

    Article  CAS  Google Scholar 

  23. Stenberg, K., Bollok, M., Reczey, K., Galbe, M., & Zacchi, G. (2000). Biotechnology and Bioengineering, 68, 204–210.

    Article  CAS  Google Scholar 

  24. Sassner, P., Galbe, M., & Zacchi, G. (2006). Enzyme and Microbial Technology, 39, 756–762.

    Article  CAS  Google Scholar 

  25. Öhgren, K., Vehmaanpera, J., Siika-Aho, M., Galbe, M., Viikari, L., & Zacchi, G. (2006). Enzyme and Microbial Technology EMT-7322.

  26. Alfani, F., Gallifuoco, A., Saporosi, A., Spera, A., & Cantarella, M. (2000). Journal of Industrial Microbiology and Biotechnology, 25, 184–192.

    Article  CAS  Google Scholar 

  27. Zhu, S. D., Wu, Y. X., Yu, Z. N., Zhang, X., Wang, C. W., Yu, F. Q., & Jin, S. W. (2006). Process Biochemistry, 41, 869–873.

    Article  CAS  Google Scholar 

Download references

Acknowledgment

We thank Novozymes A/S, Bagsvaerd, Denmark, for supplying the enzymes Celluclast and Novozyme 188, and Thomas Andersen from BioCentrum for the technical help.

Author information

Author notes

  1. Tania I. Georgieva

    Present address: Fluxome Science, Diplomvej 378, 2800, Lyngby, Denmark

  2. Troels Hilstrøm

    Present address: BioGasol ApS, DTU, Building 205, 2800, Lyngby, Denmark

Affiliations

  1. BioScience and Technology Group, BioCentrum–DTU, Technical University of Denmark, Building 227, 2800, Lyngby, Denmark

    Tania I. Georgieva, Xiaoru Hou, Troels Hilstrøm & Birgitte K. Ahring

Authors
  1. Tania I. Georgieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaoru Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Troels Hilstrøm
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Birgitte K. Ahring
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Birgitte K. Ahring.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Georgieva, T.I., Hou, X., Hilstrøm, T. et al. Enzymatic Hydrolysis and Ethanol Fermentation of High Dry Matter Wet-Exploded Wheat Straw at Low Enzyme Loading. Appl Biochem Biotechnol 148, 35–44 (2008). https://doi.org/10.1007/s12010-007-8085-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-007-8085-z

Keywords

  • Saccharomyces cerevisiae
  • Simultaneous saccharification and fermentation
  • Wet explosion
  • Pretreatment
  • Wheat straw
  • High dry matter
  • Ethanol