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Solid-state Fermentation of Xylanase from Penicillium canescens 10–10c in a Multi-layer-packed Bed Reactor

Applied Biochemistry and Biotechnology volume 145, pages 87–98 (2008)Cite this article

Abstract

Xylanase is produced by Penicillium canescens 10–10c from soya oil cake in static conditions using solid-state fermentation. The impact of several parameters such as the nature and the size of inoculum, bed-loading, and aeration is evaluated during the fermentation process. Mycelial inoculum gives more production than conidial inoculum. Increasing the quantity of inoculum enhances slightly xylanase production. Forced aeration induces more sporulation of strain and reduces xylanase production. However, forced moistened air improves the production compared to production obtained with forced dry air. In addition, increasing bed-loading reduces the specific xylanase production likely due to the incapacity of the Penicillium strain to grow deeply in the fermented soya oil cake mass. Thus, the best cultivation conditions involve mycelial inoculum form, a bed loading of 1-cm height and passive aeration. The maximum xylanase activity is obtained after 7 days of fermentation and attains 10,200 U/g of soya oil cake. These levels are higher than those presented in the literature and, therefore, show all the potentialities of this stock and this technique for the production of xylanase.

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Acknowledgements

We are very grateful to Quentin Denis (Unité de Chimie Analytique) for his helpful assistance in ergosterol analysis and Adeline Gillet (Unité de statistique, Informatique et Mathématique apliquées) for the councils on the ANOVA test of our data. We also thank the Government of the Côte d’Ivoire for financial assistance.

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Affiliations

  1. Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, 2, Passage des Déportés, Gembloux, 5030, Belgium

    Antoine A. Assamoi, Jacqueline Destain, Frank Delvigne & Philippe Thonart

  2. Unité de Chimie Analytique, Faculté Universitaire des Sciences Agronomiques de Gembloux, 2, Passage des Déportés, Gembloux, 5030, Belgium

    Georges Lognay

Authors
  1. Antoine A. Assamoi
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  2. Jacqueline Destain
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  3. Frank Delvigne
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  4. Georges Lognay
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  5. Philippe Thonart
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Correspondence to Jacqueline Destain.

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Assamoi, A.A., Destain, J., Delvigne, F. et al. Solid-state Fermentation of Xylanase from Penicillium canescens 10–10c in a Multi-layer-packed Bed Reactor. Appl Biochem Biotechnol 145, 87–98 (2008). https://doi.org/10.1007/s12010-007-8077-z

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  • DOI: https://doi.org/10.1007/s12010-007-8077-z

Keywords

  • Multi-layer-packed bed reactor
  • Penicillium canescens
  • Solid-state fermentation
  • Soya oil cake
  • Xylanase