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Fermentation of Acid-pretreated Corn Stover to Ethanol Without Detoxification Using Pichia stipitis

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Abstract

In this work, the effect of adaptation on P. stipitis fermentation using acid-pretreated corn stover hydrolyzates without detoxification was examined. Two different types of adaptation were employed, liquid hydrolyzate and solid state agar adaptation. Fermentation of 12.5% total solids undetoxified acid-pretreated corn stover was performed in shake flasks at different rotation speeds. At low rotation speed (100 rpm), both liquid hydrolyzate and solid agar adaptation highly improved the sugar consumption rate as well as ethanol production rate compared to the wild-type strains. The fermentation rate was higher for solid agar-adapted strains compared to liquid hydrolyzate-adapted strains. At a higher rotation speed (150 rpm), there was a faster sugar consumption and ethanol production for both the liquid-adapted and the wild-type strains. However, improvements in the fermentation rate between the liquid-adapted and wild strains were less pronounced at the high rotation speed.

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Acknowledgment

This investigation was funded by the US DOE project managed by Abengoa Bioenergy R&D. The authors thank the sponsors for their support.

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  1. Novozymes North America, 77 Perry Chapel Church Road, P.O. Box 576, Franklinton, NC, 27525, USA

    Frank K. Agbogbo, Frank D. Haagensen, David Milam & Kevin S. Wenger

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  1. Frank K. Agbogbo
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  2. Frank D. Haagensen
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  3. David Milam
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  4. Kevin S. Wenger
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Correspondence to Frank K. Agbogbo or Frank D. Haagensen.

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Agbogbo, F.K., Haagensen, F.D., Milam, D. et al. Fermentation of Acid-pretreated Corn Stover to Ethanol Without Detoxification Using Pichia stipitis . Appl Biochem Biotechnol 145, 53–58 (2008). https://doi.org/10.1007/s12010-007-8056-4

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  • DOI: https://doi.org/10.1007/s12010-007-8056-4

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