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Bioethanol Production from Uncooked Raw Starch by Immobilized Surface-engineered Yeast Cells

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Abstract

Surface-engineered yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis α-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. By using 50 g/L cells during batch fermentation, ethanol concentration could reach 53 g/L in 7 days. During repeated batch fermentation, the production of ethanol could be maintained for seven consecutive cycles. For cells immobilized in loofa sponge, the concentration of ethanol could reach 42 g/L in 3 days in a circulating packed-bed bioreactor. However, the production of ethanol stopped thereafter because of limited contact between cells and starch. The bioreactor could be operated for repeated batch production of ethanol, but ethanol concentration dropped to 55% of its initial value after five cycles because of a decrease in cell mass and cell viability in the bioreactor. Adding cells to the bioreactor could partially restore ethanol production to 75% of its initial value.

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Authors and Affiliations

  1. Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan, 333, Taiwan, Republic of China

    Jyh-Ping Chen & Kuo-Wei Wu

  2. Division of Molecular Science, Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe, 657-8501, Japan

    Hideki Fukuda

Authors
  1. Jyh-Ping Chen
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  2. Kuo-Wei Wu
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  3. Hideki Fukuda
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Correspondence to Jyh-Ping Chen.

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Chen, JP., Wu, KW. & Fukuda, H. Bioethanol Production from Uncooked Raw Starch by Immobilized Surface-engineered Yeast Cells. Appl Biochem Biotechnol 145, 59–67 (2008). https://doi.org/10.1007/s12010-007-8054-6

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  • DOI: https://doi.org/10.1007/s12010-007-8054-6

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