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Xylanase Production by Bacillus circulans D1 Using Maltose as Carbon Source

Applied Biochemistry and Biotechnology volume 146, pages 29–37 (2008)Cite this article

Abstract

Bacillus circulans D1 is a good producer of extracellular thermostable xylanase. Xylanase production in different carbon sources was evaluated and the enzyme synthesis was induced by various carbon sources. It was found that d-maltose is the best inducer of the enzyme synthesis (7.05 U/mg dry biomass at 48 h), while d-glucose and d-arabinose lead to the production of basal levels of xylanase. The crude enzyme solution is free of cellulases, even when the microorganism was cultivated in a medium with d-cellobiose. When oat spelt xylan was supplemented with d-glucose, the repressive effect of this sugar on xylanase production was observed at 24 h, only when used at 5.0 g/L, leading to a reduction of 60% on the enzyme production. On the other hand, when the xylan medium was supplemented with d-xylose (3.0 or 5.0 g/L), this effect was more evident (80 and 90% of reduction on the enzyme production, respectively). Unlike that observed in the xylan medium, glucose repressed xylanase production in the maltose medium, leading to a reduction of 55% on the enzyme production at 24 h of cultivation. Xylose, at 1.0 g/L, induced xylanase production on the maltose medium. On this medium, the repressive effect of xylose, at 3.0 or 5.0 g/L, was less expressive when compared to its effect on the xylan medium.

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Acknowledgments

Daniela Alonso Bocchini is grateful for the Ph.D. fellowship funded by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo, São Paulo, Brazil). The authors also acknowledge the financial support of FAPESP and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil).

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  1. Laboratório de Bioquímica e Microbiologia Aplicada, IBILCE—Instituto de Biociências Letras e Ciências Exatas, UNESP—Universidade Estadual Paulista, Rua Cristóvão Colombo, 2265, São José do Rio Preto, São Paulo, CEP 15054-000, Brazil

    D. A. Bocchini, E. Gomes & R. Da Silva

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  1. D. A. Bocchini
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  2. E. Gomes
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  3. R. Da Silva
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Correspondence to R. Da Silva.

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Bocchini, D.A., Gomes, E. & Da Silva, R. Xylanase Production by Bacillus circulans D1 Using Maltose as Carbon Source. Appl Biochem Biotechnol 146, 29–37 (2008). https://doi.org/10.1007/s12010-007-8051-9

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  • DOI: https://doi.org/10.1007/s12010-007-8051-9

Keywords

  • Xylanase
  • Maltose
  • Induction
  • Repression
  • Bacillus circulans