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Developments in Directed Evolution for Improving Enzyme Functions

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Abstract

The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCHY), recombined extension on truncated templates (RETT), and so on have been developed to mimic and accelerate nature’s recombination strategy. This review discusses gradual advances in the techniques and strategies used for the directed evolution of biocatalytic enzymes aimed at improving the quality and potential of enzyme libraries, their advantages, and disadvantages.

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Authors and Affiliations

  1. Centre for the Environment, Indian Institute of Technology (IIT)–Guwahati, Guwahati, 781039, Assam, India

    S. Sen, V. Venkata Dasu & B. Mandal

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  1. S. Sen
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  2. V. Venkata Dasu
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  3. B. Mandal
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Correspondence to V. Venkata Dasu.

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Submitted to Applied Biochemistry and Biotechnology

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Sen, S., Venkata Dasu, V. & Mandal, B. Developments in Directed Evolution for Improving Enzyme Functions. Appl Biochem Biotechnol 143, 212–223 (2007). https://doi.org/10.1007/s12010-007-8003-4

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  • DOI: https://doi.org/10.1007/s12010-007-8003-4

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