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Optimization of Fermentation Parameters to Enhance the Production of Ethanol from Palmyra Jaggery Using Saccharomyces cerevisiae in a Batch Fermentor

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Abstract

Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett–Burman design, impeller speed, concentrations of CoCl2 and KH2PO4 were identified as significant variables, which highly influenced ethanol production, and these variables were further optimized using a central composite design (CCD). The ethanol production was adequately approximated with a full quadratic equation obtained from three factors and five levels of CCD. Maximum ethanol concentration of 132.56 g/l (16.8% [v/v]) was obtained for an impeller speed of 247.179 (≈250) rev/min, CoCl2 of 0.263 g/l and KH2PO4 of 2.39 g/l. A second-order polynomial regression model was fitted and was found adequate with R 2 of 0.8952. This combined statistical approach enables rapid identification and investigation of significant parameters for improving the ethanol production and could be very useful in optimizing processes.

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Authors and Affiliations

  1. Department of Neurology, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Pathology 233/Meyer 222, Baltimore, MD, 21287, USA

    Veera Venkata Ratnam Bandaru

  2. RITAMS Degree College, Andhra University, West Godavari District, Tanuku, Andhra Pradesh, 534211, India

    Sita Ramalakshmi Bandaru

  3. Center for Biotechnology, Department of Chemical Engineering, College of Engineering, Andhra University, Visakhapatnam, 530 003, India

    Sita Ramalakshmi Bandaru, Subba Rao Somalanka, Sarat Babu Imandi, Srinivasa Rao Bejawada, Narasimha Rao Medicherla, Jayaraju Karothi & Ayyanna Chityala

  4. Bioanalytical Core Laboratory, General Clinical Research Center, Georgetown University, Washington, DC, USA

    Damodara Rao Mendu

  5. Department of Botany, Sri Krishnadevaraya University, Anantapur, 515003, India

    Thangadurai Devarajan

  6. Al-Ameer College of Engineering and Technology, Gudilova, Anandapuram, Visakhapatnam, India

    Srinivasa Rao Bejawada & Ayyanna Chityala

  7. Department of Bio-Technology, Acharya Nagarjuna University, Nagarjuna Nagar, 522 510, India

    Srinivasa Rao Bejawada

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  1. Veera Venkata Ratnam Bandaru
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  2. Sita Ramalakshmi Bandaru
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  3. Subba Rao Somalanka
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  4. Damodara Rao Mendu
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  5. Sarat Babu Imandi
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  6. Srinivasa Rao Bejawada
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  7. Narasimha Rao Medicherla
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  8. Thangadurai Devarajan
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  9. Jayaraju Karothi
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  10. Ayyanna Chityala
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Correspondence to Veera Venkata Ratnam Bandaru.

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Bandaru, V.V.R., Bandaru, S.R., Somalanka, S.R. et al. Optimization of Fermentation Parameters to Enhance the Production of Ethanol from Palmyra Jaggery Using Saccharomyces cerevisiae in a Batch Fermentor. Appl Biochem Biotechnol 143, 224–235 (2007). https://doi.org/10.1007/s12010-007-0048-x

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  • DOI: https://doi.org/10.1007/s12010-007-0048-x

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