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Can rhBMP-2 Containing Collagen Sponges Enhance Bone Repair in Ovariectomized Rats?: A Preliminary Study

  • Symposium: Tribute to Dr. Marshall Urist: Musculoskeletal Growth Factors
  • Published:
Clinical Orthopaedics and Related Research®

Abstract

With an aging population the frequency of postmenopausal fractures is increasing. Methods to enhance the repair of osteoporotic bone repair therefore become more important to reduce the society burden of care. We asked if absorbable collagen sponges containing recombinant human bone morphogenetic protein-2 (rhBMP-2) have the potential to enhance bone repair. We randomly assigned 40 rats into the ovariectomy and sham operation groups. A segmental defect was created in the right tibia 12 weeks after ovariectomy. rhBMP-2-containing absorbable collagen sponges were implanted into the defect in half of the animals in each group. We analyzed radiographs and histological sections and performed three-point bending tests to assess repair. Radiological scores in the rhBMP-2 applied rats were higher than those in controls at the end of 8 weeks after tibial osteotomy. The specimens failed under higher loads in the rhBMP-2-applied groups and histology revealed a higher fracture healing score, including callus formation, bone union, marrow changes, and cortex remodeling. We observed no adverse tissue responses such as fibrous connective tissue formation and inflammatory cellular infiltration. rhBMP-2 in absorbable collagen sponges enhanced bone repair in segmental tibial defects of ovariectomized rats. The sponges with rhBMP-2 appeared to enhance bone repair.

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Acknowledgments

We thank Dr. Hasan Bilgili of Ankara University for conducting the ovariectomies; Dr. Sedat Ulger for evaluating the radiological images; Dr. Tevfik Sabuncu, Dr. Müge Harma, and Dr. Mehmet Harma of Harran University for their help in creating the osteoporosis rat model; Arzu Tasci of Middle East Technical University for assisting with the biomechanical tests and Dr. Sedat Isikli of Hacettepe University for statistical analysis. Orhan Resit Cubukcu MD and Celal Aydin MD of Middle East Technical University Medical Center Radiodiagnostic Unit independently evaluated and scored the xray pictures. Dilara Zeybek MD of Hacettepe University Faculty of Medicine, Department of Histology and Embryology, assisted blinded evaluation and scoring of the histological sections.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Harran University Faculty of Medicine, Sanliurfa, Turkey

    Sezgin Sarban MD & U. Erdem Isikan MD

  2. Department of Orthopaedic Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    Alparslan Senkoylu MD

  3. Department of Histology and Embryology, Hacettepe University Faculty of Medicine, Ankara, Turkey

    Petek Korkusuz MD, PhD

  4. Department of Biomedical Engineering, Institute of Natural Sciences, Middle East Technical University, 100 Yil, Ankara, 06531, Turkey

    Feza Korkusuz MD

Authors
  1. Sezgin Sarban MD
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  2. Alparslan Senkoylu MD
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  3. U. Erdem Isikan MD
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  4. Petek Korkusuz MD, PhD
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  5. Feza Korkusuz MD
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Corresponding author

Correspondence to Feza Korkusuz MD.

Additional information

This project was partly supported by Harran University Research Fund (HUBAK 436). Kurtsan Inc. (Istanbul, Turkey) supplied the absorbable collagen sponges for this study.

Each author certifies that his or her institution has approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.

This work was performed at the Middle East Technical University.

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Sarban, S., Senkoylu, A., Isikan, U.E. et al. Can rhBMP-2 Containing Collagen Sponges Enhance Bone Repair in Ovariectomized Rats?: A Preliminary Study. Clin Orthop Relat Res 467, 3113–3120 (2009). https://doi.org/10.1007/s11999-009-1004-6

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  • DOI: https://doi.org/10.1007/s11999-009-1004-6

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