Abstract
This work presented a novel strategy to integrate colorant and stabilizing effects of BSA-crocin I (BC) nanoparticles with gel-like structure of high internal phase Pickering emulsion (HIPPE, φ = 0.75). The BSA was cross-lined with crocin I to obtain BC nanoparticles for stabilizing HIPPE. When pH = 7.4, the particle size of BC nanoparticles was 326.65–366.40 nm with low polydispersity index (PDI) around 0.5 and zeta potential around −8 mV. The three-phase contact angle of BC nanoparticles was approaching 90°, indicating the strong absorptive capacity at the O/W interface. CLSM images showed the BC nanoparticles surrounded the oil droplets in the emulsions. Specifically, BC-1.5 (7.5% BSA and 0.1125% crocin I)-stabilized HIPPE exhibited the highest stability, with a Turbiscan stability index (TSI) value of 1.3. The thixotropic recovery degree of BC-1.5-stabilized HIPPE was the highest, while the G0′ and G0″ were the lowest, which confirmed that BC-1.5 constructed the most compact gel-network structure around emulsion droplets. The color difference of BC-1.5-stabilized HIPPE was much lower than 1 throughout the UV treatment with the lowest oxidation rate of gardenia oil. The colorimetric and oxidative results verified the color-protective and anti-oxidative abilities of BC nanoparticle-stabilized HIPPE, which might be used for the improvement of the meat product.
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This work was supported by the Key Research and Development Program of Zhejiang province (2021C02014) and Wenzhou Agricultural Development Research Program (2018ZN0001).
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Hao Huang and Yingjie Zhu designed and conducted experiments. Hao Huang and Yingjie Zhu wrote the main manuscript text. Hao Huang prepared figures and tables. Li Li, Hailong Yang, Guangsheng Zhao, and Zisheng Luo revised the manuscript. All authors reviewed the manuscript.
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Huang, H., Zhu, Y., Li, L. et al. Cross-Linked Bovine Serum Albumin-Crocin I Nanoparticle-Based Gel Network for Stabilizing High Internal Phase Pickering Emulsion. Food Bioprocess Technol 15, 2573–2586 (2022). https://doi.org/10.1007/s11947-022-02903-3
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DOI: https://doi.org/10.1007/s11947-022-02903-3