Gamma oryzanol (γ-oryzanol), a bioactive ingredient in rice bran oil, has potential as a nutraceutical, pharmaceutical, and cosmeceutical. γ-Oryzanol is normally degraded through oxidation by oxygen and sunlight. Microencapsulation is a good alternative to preserve the organoleptic properties, antioxidants and bioactive compounds, and oxidative degradation of γ-oryzanol in rice bran oil. Thus, this study aimed to study and optimize the microencapsulation of rice bran oil by spray drying. The effects of carrier composition (mixtures of maltodextrin (MD) with whey protein concentrate (WPC), from 1 to 4 of MD, or sodium caseinate (NaCas), from 6 to 10 of MD), homogenization pressure (150-300 bar), and carrier to oil ration (1-4) on microencapsulation efficiency and yield, bulk density and moisture content of microcapsules were investigated. To optimize the rice bran oil microencapsulation, a carrier composed of MD and NaCas (8:1), moderate pressures (200–250 bar), and carrier to oil ratios of 3:1 were required. The significant influences of these factors on microencapsulation efficiency and yield were found. Meanwhile, the bulk density and moisture of microcapsules were only affected by carrier mixtures. The results of microencapsulation efficiency and yield of γ-oryzanol that obtained in optimal conditions were 85.23% and 72.12%, respectively. Microencapsulation was a potential method for protection of γ-oryzanol in rice bran oil.
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Aghbashlo, M., Mobli, H., Madadlou, A., & Rafiee, S. (2013). Influence of wall material and inlet drying air temperature on the microencapsulation of fish oil by spray drying. Food and Bioprocess Technology, 6(6), 1561–1569.
Ali, A., & Devarajan, S. (2017). Nutritional and health benefits of rice bran oil. In Brown Rice (pp. 135–158). Springer.
Amiri Samani, S., & Naji, M. H. (2019). Effect of homogenizer pressure and temperature on physicochemical, oxidative stability, viscosity, droplet size, and sensory properties of sesame vegetable cream. Food Science & Nutrition, 7(3), 899–906.
AOAC. (2000). Determination of moisture content.
Bae, E. K., & Lee, S. J. (2008). Microencapsulation of avocado oil by spray drying using whey protein and maltodextrin. Journal of Microencapsulation, 25(8), 549–560. https://doi.org/10.1080/02652040802075682
Bakry, A. M., Abbas, S., Ali, B., Majeed, H., Abouelwafa, M. Y., Mousa, A., & Liang, L. (2016). Microencapsulation of oils: A comprehensive review of benefits, techniques, and applications. Comprehensive Reviews in Food Science and Food Safety. https://doi.org/10.1111/1541-4337.12179
Bastıoğlu, A. Z., Koç, M., & Ertekin, F. K. (2017). Moisture sorption isotherm of microencapsulated extra virgin olive oil by spray drying. Journal of Food Measurement and Characterization, 11(3), 1295–1305.
Benito-Román, Sanz, T., & Beltrán, S. (2020). Microencapsulation of rice bran oil using pea protein and maltodextrin mixtures as wall material. Heliyon, 6(4). https://doi.org/10.1016/j.heliyon.2020.e03615
Bhusari, S. N., Muzaffar, K., & Kumar, P. (2014). Effect of carrier agents on physical and microstructural properties of spray dried tamarind pulp powder. Powder Technology, 266, 354–364.
Böger, B. R., Georgetti, S. R., & Kurozawa, L. E. (2018). Microencapsulation of grape seed oil by spray drying. Food Science and Technology, (AHEAD).
Bumrungpert, A., Chongsuwat, R., Phosat, C., & Butacnum, A. (2019). Rice bran oil containing gamma-oryzanol improves lipid profiles and antioxidant status in hyperlipidemic subjects: A randomized double-blind controlled trial. The Journal of Alternative and Complementary Medicine, 25(3), 353–358.
Carneiro, H. C. F., Tonon, R. V., Grosso, C. R. F., & Hubinger, M. D. (2013). Encapsulation efficiency and oxidative stability of flaxseed oil microencapsulated by spray drying using different combinations of wall materials. Journal of Food Engineering, 115(4), 443–451.
Comunian, T. A., da Silva Anthero, A. G., Bezerra, E. O., Moraes, I. C. F., & Hubinger, M. D. (2020). Encapsulation of pomegranate seed oil by emulsification followed by spray drying: Evaluation of different biopolymers and their effect on particle properties. Food and Bioprocess Technology, 13(1), 53–66.
Domian, E., Brynda-Kopytowska, A., & Marzec, A. (2017). Functional properties and oxidative stability of flaxseed oil microencapsulated by spray drying using legume proteins in combination with soluble fiber or trehalose. Food and Bioprocess Technology, 10(7), 1374–1386.
Drapala, K. P., Auty, M. A. E., Mulvihill, D. M., & O’Mahony, J. A. (2017). Influence of emulsifier type on the spray-drying properties of model infant formula emulsions. Food Hydrocolloids, 69, 56–66.
Drusch, S., & Berg, S. (2008). Extractable oil in microcapsules prepared by spray-drying: Localisation, determination and impact on oxidative stability. Food Chemistry, 109(1), 17–24.
Fäldt, P., & Bergenståhl, B. (1996a). Spray-dried whey protein/lactose/soybean oil emulsions. 1. Surface composition and particle structure. Food Hydrocolloids, 10(4), 421–429.
Fäldt, P., & Bergenståhl, B. (1996b). Spray-dried whey protein/lactose/soybean oil emulsions. 2. Redispersability, wettability and particle structure. Food Hydrocolloids, 10(4), 431–439.
Floury, J., Desrumaux, A., & Lardières, J. (2000). Effect of high-pressure homogenization on droplet size distributions and rheological properties of model oil-in-water emulsions. Innovative Food Science and Emerging Technologies, 1(2), 127–134. https://doi.org/10.1016/S1466-8564(00)00012-6
Fraterrigo Garofalo, S., Tommasi, T., & Fino, D. (2020). A short review of green extraction technologies for rice bran oil. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-020-00846-3
Gupta, P. K., Jadhav, S. B., & Singhal, R. S. (2015). Development of shrikhand premix using microencapsulated rice bran oil as fat alternative and hydrocolloids as texture modifier. Food Hydrocolloids, 48, 220–227.
Hogan, S. A., McNamee, B. F., Dolores O’Riordan, E., & O’Sullivan, M. (2001). Microencapsulating properties of sodium caseinate. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf000276q
Hunt, J. A., & Dalgleish, D. G. (1994). Adsorption behaviour of whey protein isolate and caseinate in soya oil-in-water emulsions. Food Hydrocolloids, 8(2), 175–187.
Jafari, S. M., Assadpoor, E., He, Y., & Bhandari, B. (2008). Encapsulation efficiency of food flavours and oils during spray drying. Drying Technology, 26(7), 816–835. https://doi.org/10.1080/07373930802135972
Jamshidi, A., Antequera, T., Solomando, J. C., & Perez-Palacios, T. (2020). Microencapsulation of oil and protein hydrolysate from fish within a high-pressure homogenized double emulsion. Journal of Food Science and Technology, 57(1), 60–69.
Joshi, M., Kaur, R., Kanwar, P., Dhiman, G., Sharma, G., Lata, S., et al. (2016). To evaluate antioxidant activity of γ–oryzanol extracted from rice bran oil. International Journal of Life science and Pharma Research, 6, 17–25.
Kaur, A., Jassal, V., Thind, S. S., & Aggarwal, P. (2012). Rice bran oil an alternate bakery shortening. Journal of Food Science and Technology, 49(1), 110–114. https://doi.org/10.1007/s13197-011-0259-6
Krist, S. (2020). Rice bran oil. In Vegetable Fats and Oils (pp. 641–646). Springer.
Kshirsagar, A. C., Yenge, V. B., Sarkar, A., & Singhal, R. S. (2009). Efficacy of pullulan in emulsification of turmeric oleoresin and its subsequent microencapsulation. Food Chemistry, 113(4), 1139–1145.
Kubo, M. T. K., Augusto, P. E. D., & Cristianini, M. (2013). Effect of high pressure homogenization (HPH) on the physical stability of tomato juice. Food Research International, 51(1), 170–179.
Lai, O.-M., Jacoby, J. J., Leong, W.-F., & Lai, W.-T. (2019). Nutritional studies of rice bran oil. Rice Bran and Rice Bran Oil, 19–54.
Lavanya, M. N., Kathiravan, T., Moses, J. A., & Anandharamakrishnan, C. (2019). Influence of spray-drying conditions on microencapsulation of fish oil and chia oil. Drying Technology.
Lee, S., Yu, S., Park, H. J., Jung, J., Go, G., & Kim, W. (2019). Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages. PloS one, 14(10), e0222857.
Liu, R., Liu, R., Shi, L., Zhang, Z., Zhang, T., Lu, M., et al. (2019). Effect of refining process on physicochemical parameters, chemical compositions and in vitro antioxidant activities of rice bran oil. LWT, 109, 26–32.
McNamee, B. F., O’Riorda, E. D., & O’Sullivan, M. (1998). Emulsification and microencapsulation properties of gum arabic. Journal of Agricultural and Food Chemistry, 46(11), 4551–4555.
Mohammed, N. K., Tan, C. P., Manap, Y. A., Muhialdin, B. J., & Hussin, A. S. M. (2020). Spray drying for the encapsulation of oils—A review. Molecules. https://doi.org/10.3390/molecules25173873
Murali, S., Kar, A., & Patel, A. S. (2020). Protein–polysaccharide matrix as novel wall material for rice bran oil encapsulation. Journal of Agricultural Engineering, 57(4), 329–340.
Murali, S., Kar, A., Patel, A. S., Kumar, J., Mohapatra, D., & Dash, S. K. (2016). Encapsulation of rice bran oil in tapioca starch-soya protein isolate complex using spray drying. Indian Journal of Agricultural Sciences, 86(8), 984–991.
Murali, S., Kar, A., Patel, A. S., Mohapatra, D., & Krishnakumar, P. (2017). Optimization of rice bran oil encapsulation using jackfruit seed starch–whey protein isolate blend as wall material and its characterization. International Journal of Food Engineering, 13(4).
Ochoa, A. A., Hernández-Becerra, J. A., Cavazos-Garduño, A., Vernon-Carter, E. J., & GarcÍa, H. S. (2016). Preparation and characterization of curcumin nanoemulsions obtained by thin-film hydration emulsification and ultrasonication methods. Revista Mexicana De Ingeniera Quimica, 15(1), 79–90.
Oliveira, É. R., Fernandes, R. V. B., Botrel, D. A., Carmo, E. L., Borges, S. V., & Queiroz, F. (2018). Study of different wall matrix biopolymers on the properties of spray-dried pequi oil and on the stability of bioactive compounds. Food and Bioprocess Technology, 11(3), 660–679.
Onsaard, E., & Onsaard, W. (2019). Microencapsulated vegetable oil powder. In Microencapsulation-Processes, Technologies and Industrial Applications. IntechOpen.
Ozdikicierler, O., Dirim, S. N., & Pazir, F. (2014). The effects of spray drying process parameters on the characteristic process indices and rheological powder properties of microencapsulated plant (Gypsophila) extract powder. Powder Technology, 253, 474–480.
Pal, Y. P., & Pratap, A. P. (2017). Rice bran oil: A versatile source for edible and industrial applications. Journal of Oleo Science, 66(6), 551–556.
Park, C. W., & Drake, M. (2017). The effect of homogenization pressure on the flavor and flavor stability of whole milk powder. Journal of Dairy Science, 100(7), 5195–5205.
Punia, S., Kumar, M., & Sandhu, K. S. (2021). Rice bran oil: An emerging source of functional oil. Journal of Food Processing and Preservation, e15318.
Rodea-González, D. A., Cruz-Olivares, J., Román-Guerrero, A., Rodríguez-Huezo, M. E., Vernon-Carter, E. J., & Pérez-Alonso, C. (2012). Spray-dried encapsulation of chia essential oil (Salvia hispanica L.) in whey protein concentrate-polysaccharide matrices. Journal of Food Engineering, 111(1), 102–109.
Sanchez-Reinoso, Z., Osorio, C., & Herrera, A. (2017). Effect of microencapsulation by spray drying on cocoa aroma compounds and physicochemical characterisation of microencapsulates. Powder Technology, 318, 110–119.
Sarabandi, K., Peighambardoust, S. H., Mahoonak, A. R. S., & Samaei, S. P. (2018). Effect of different carriers on microstructure and physical characteristics of spray dried apple juice concentrate. Journal of Food Science and Technology, 55(8), 3098–3109.
Shu, B., Yu, W., Zhao, Y., & Liu, X. (2006). Study on microencapsulation of lycopene by spray-drying. Journal of Food Engineering, 76(4), 664–669. https://doi.org/10.1016/j.jfoodeng.2005.05.062
Silva, V. M., Vieira, G. S., & Hubinger, M. D. (2014). Influence of different combinations of wall materials and homogenisation pressure on the microencapsulation of green coffee oil by spray drying. Food Research International, 61, 132–143.
Sookwong, P., & Mahatheeranont, S. (2017). Supercritical CO2 extraction of rice bran oil—The technology, manufacture, and applications. Journal of oleo science, ess17019.
Srisaipet, A., & Nuddagul, M. (2014). Influence of temperature on gamma-oryzanol stability of edible rice bran oil during heating. International Journal of Chemical Engineering and Applications, 5(4), 303.
Takeungwongtrakul, S., Benjakul, S., & Aran, H. (2014). Micro-encapsulation of Pacific white shrimp oil as affected by emulsification condition. Food Science and Human Wellness, 3(3–4), 175–182.
Tonon, R. V., Pedro, R. B., Grosso, C. R. F., & Hubinger, M. D. (2012). Microencapsulation of flaxseed oil by spray drying: Effect of oil load and type of wall material. Drying Technology, 30(13), 1491–1501. https://doi.org/10.1080/07373937.2012.696227
Vega, C., Kim, E.H.J., Chen, X. D., & Roos, Y. H. (2005). Solid-state characterization of spray-dried ice cream mixes. Colloids and Surfaces b: Biointerfaces, 45(2), 66–75.
Vidović, S. S., Vladić, J. Z., Vaštag, ŽG., Zeković, Z. P., & Popović, L. M. (2014). Maltodextrin as a carrier of health benefit compounds in Satureja montana dry powder extract obtained by spray drying technique. Powder Technology, 258, 209–215.
Wang, W., Jiang, Y., & Zhou, W. (2013). Characteristics of soy sauce powders spray-dried using dairy whey proteins and maltodextrins as drying aids. Journal of Food Engineering, 119(4), 724–730.
Wang, Y. (2019). Applications of rice bran oil. Chemistry, Processing and Utilization. Elsevier Inc. https://doi.org/10.1016/B978-0-12-812828-2.00006-8
Wong, S. K., Kamisah, Y., Mohamed, N., Muhammad, N., Masbah, N., Fahami, N. A. M., et al. (2020). Potential role of tocotrienols on non-communicable diseases: A review of current evidence. Nutrients, 12(1), 259.
Young, S. L., Sarda, X., & Rosenberg, M. (1993). Microencapsulating properties of whey proteins. 1. Microencapsulation of anhydrous milk fat. Journal of Dairy Science, 76(10), 2868–2877.
Yu, Y., Zhang, J., Wang, J., & Sun, B. (2019). The anti-cancer activity and potential clinical application of rice bran extracts and fermentation products. RSC Advances, 9(31), 18060–18069.
Yuan, L., Sun, T., Hu, H., Yuan, S., Yang, Y., Wang, R., et al. (2019). Preparation and characterization of microencapsulated ethylenediamine with epoxy resin for self-healing composites. Scientific Reports, 9(1), 1–10.
Zhang, Y., Pan, K., & Zhong, Q. (2018). Eugenol nanoencapsulated by sodium caseinate: Physical, antimicrobial, and biophysical properties. Food Biophysics, 13(1), 37–48.
We acknowledge the support of time and facilities from Ho Chi Minh City University of Technology (HCMUT), VNU-HCM for this study.
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Lai, Q.D., Doan, N.T.T. & Nguyen, T.T.T. Influence of Wall Materials and Homogenization Pressure on Microencapsulation of Rice Bran Oil. Food Bioprocess Technol 14, 1885–1896 (2021). https://doi.org/10.1007/s11947-021-02685-0
- Rice bran oil
- Wall material