Abstract
Microalgal biomass is a promising functional ingredient for innovative food products due to its potential health benefits given by its composition (protein, minerals, vitamins, pigments, fatty acids, sterol and antioxidants). However, in practice, the level of incorporation of microalgae in many products is limited due to among others the strong green colour. In this study, we investigated the potential of 3D food printing to incorporate microalgae in cereal snacks. Chlorella vulgaris and Arthrospira platensis were the microalgae evaluated. First, the effect of microalgae fortification on both the rheological properties and printability of batters and on the properties of snacks (i.e. shape, texture and colour) was studied. Microalgae fortification improved the printability of batters using extrusion-based 3D printing, which was concluded from the increased extrusion force and shear modulus in comparison to those for the batter without microalgae. Subsequently, snacks enriched with 3% and 4% Chlorella provided most accurate printed structures. However, snacks with the latter levels of microalgae addition are probably not well accepted by consumers due to the strong green and dark colour of the cereal snacks after baking. The next logical step could be to use coaxial food printing to hide the microalgae inside the snack. First coaxial printing experiments showed that this could be a feasible approach.
References
Andrade, L. M. (2018). Chlorella and Spirulina microalgae as sources of functional foods, nutraceuticals, and food supplements; an overview. MOJ Food Processing & Technology, 6(1), 45–58. https://doi.org/10.15406/mojfpt.2018.06.00144.
AOAC. (2005). Official methods of analysis of AOAC International. Association of Official Analysis Chemists International.
Barkallah, M., Dammak, M., Louati, I., Hentati, F., Hadrich, B., Mechichi, T., Ayadi, M. A., Fendri, I., Attia, H., & Abdelkafi, S. (2017). Effect of Spirulina platensis fortification on physicochemical, textural, antioxidant and sensory properties of yogurt during fermentation and storage. LWT - Food Science and Technology, 84, 323–330. https://doi.org/10.1016/j.lwt.2017.05.071.
Batista, A. P., Gouveia, L., Bandarra, N. M., Franco, J. M., & Raymundo, A. (2013). Comparison of microalgal biomass profiles as novel functional ingredient for food products. Algal Research, 2(2), 164–173. https://doi.org/10.1016/j.algal.2013.01.004.
Batista, A. P., Niccolai, A., Fradinho, P., Fragoso, S., Bursic, I., Rodolfi, L., Biondi, N., Tredici, M. R., Sousa, I., & Raymundo, A. (2017). Microalgae biomass as an alternative ingredient in cookies: sensory, physical and chemical properties, antioxidant activity and in vitro digestibility. Algal Research, 26(March), 161–171. https://doi.org/10.1016/j.algal.2017.07.017.
Batista, A. P., Niccolai, A., Bursic, I., Sousa, I., Raymundo, A., Rodolfi, L., Biondi, N., & Tredici, M. R. (2019). Microalgae as functional ingredients in savory food products : application to wheat crackers. Foods, 8(611), 1–22. https://doi.org/10.3390/foods8120611.
Beheshtipour, H., Mortazavian, A. M., Mohammadi, R., Sohrabvandi, S., & Khosravi-Darani, K. (2013). Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks. Comprehensive Reviews in Food Science and Food Safety, 12(2), 144–154. https://doi.org/10.1111/1541-4337.12004.
Bodart, M., de Peñaranda, R., Deneyer, A., & Flamant, G. (2008). Photometry and colorimetry characterisation of materials in daylighting evaluation tools. Building and Environment, 43(12), 2046–2058. https://doi.org/10.1016/j.buildenv.2007.12.006.
Buono, S., Langellotti, A. L., Martello, A., Rinna, F., & Fogliano, V. (2014). Functional ingredients from microalgae. Food & Function, 5(8), 1669–1685. https://doi.org/10.1039/c4fo00125g.
Cai, L., Choi, I., Hyun, J. N., Jeong, Y. K., & Baik, B. K. (2014). Influence of bran particle size on bread-baking quality of whole grain wheat flour and starch retrogradation. Cereal Chemistry, 91(1), 65–71. https://doi.org/10.1094/CCHEM-02-13-0026-R.
Caporgno, M. P., & Mathys, A. (2018). Trends in microalgae incorporation into innovative food products with potential health benefits. Frontiers in Nutrition, 5(July), 1–10. https://doi.org/10.3389/fnut.2018.00058.
Caporizzi, R., Derossi, A., & Severini, C. (2019). Cereal-based and insect-enriched printable food. Fundamentals of 3D food printing and applications. Elsevier Inc. https://doi.org/10.1016/b978-0-12-814564-7.00004-3.
Cervejeira Bolanho, B., Buranelo Egea, M., Morocho Jácome, A. L., Campos, I., Monteiro de Carvalho, J. C., & Godoy Danesi, E. D. (2014). Antioxidant and nutritional potential of cookies enriched with Spirulina platensis and sources of fibre. Journal of Food and Nutrition Research, 53(2), 171–179. https://doi.org/10.1093/humrep/deq166.
Fradique, M., Batista, A. P., Nunes, M. C., Gouveia, L., Bandarra, N. M., & Raymundo, A. (2010). Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: preparation and evaluation. Journal of the Science of Food and Agriculture, 90(10), 1656–1664. https://doi.org/10.1002/jsfa.3999.
García-Segovia, P., Pagán-Moreno, M., Lara, I., & Martínez-Monzó, J. (2017). Effect of microalgae incorporation on physicochemical and textural properties in wheat bread formulation. Food Science and Technology International, 23(5), 437–447. https://doi.org/10.1177/1082013217700259.
García-Segovia, P., García-Alcaraz, V., Balasch-Parisi, S., & Martínez-Monzó, J. (2020a). 3D printing of gels based on xanthan/konjac gums. Innovative Food Science & Emerging Technologies, 102343. https://doi.org/10.1016/j.ifset.2020.102343.
García-Segovia, P., García-Alcaraz, V., Tárrega, A., & Martínez-Monzó, J. (2020b). Consumer perception and acceptability of microalgae based breadstick. Food Science and Technology International, 26(6), 493–502. https://doi.org/10.1177/1082013220906235.
Gouveia, L., Batista, A. P., Miranda, A., Empis, J., & Raymundo, A. (2007). Chlorella vulgaris biomass used as colouring source in traditional butter cookies. Innovative Food Science & Emerging Technologies, 8(3), 433–436. https://doi.org/10.1016/J.IFSET.2007.03.026.
Gouveia, L., Batista, A. P., Sousa, I., Raymundo, A., & Bandarra, N. M. (2008). Microalgae in novel food products. In K. N. Papadopoulos (Ed.), Food chemistry research developments (pp. 1–36). Nova Science Publishers, Inc.
Graça, C., Fradinho, P., Sousa, I., & Raymundo, A. (2018). Impact of Chlorella vulgaris on the rheology of wheat flour dough and bread texture. LWT - Food Science and Technology, 89(July 2017), 466–474. https://doi.org/10.1016/j.lwt.2017.11.024.
Huang, M., Zhang, M., & Bhandari, B. (2019). Assessing the 3D printing precision and texture properties of brown rice induced by infill levels and printing variables. Food and Bioprocess Technology, 12(7), 1185–1196. https://doi.org/10.1007/s11947-019-02287-x.
Kim, H. W., Bae, H., & Park, H. J. (2018a). Reprint of: Classification of the printability of selected food for 3D printing: development of an assessment method using hydrocolloids as reference material. Journal of Food Engineering, 220, 28–37. https://doi.org/10.1016/j.jfoodeng.2017.10.023.
Kim, H. W., Lee, J. H., Park, S. M., Lee, M. H., Lee, I. W., Doh, H. S., & Park, H. J. (2018b). Effect of hydrocolloids on rheological properties and printability of vegetable inks for 3D food printing. Journal of Food Science, 83(12), 2923–2932. https://doi.org/10.1111/1750-3841.14391.
Kim, H. W., Lee, I. J., Park, S. M., Lee, J. H., Nguyen, M. H., & Park, H. J. (2019). Effect of hydrocolloid addition on dimensional stability in post-processing of 3D printable cookie dough. LWT - Food Science and Technology, 101(November 2018), 69–75. https://doi.org/10.1016/j.lwt.2018.11.019.
Kohajdová, Z., Karovičacová, J., Jurasová, M., & Kukurová, K. (2011). Application of citrus dietary fibre preparations in biscuit production. Journal of Food and Nutrition Research, 50(3), 182–190.
Lafarga, T. (2019). Effect of microalgal biomass incorporation into foods: nutritional and sensorial attributes of the end products. Algal Research, 41, 101566. https://doi.org/10.1016/j.algal.2019.101566.
Lafarga, T., Acién-Fernández, F. G., Castellari, M., Villaró, S., Bobo, G., & Aguiló-Aguayo, I. (2019). Effect of microalgae incorporation on the physicochemical, nutritional, and sensorial properties of an innovative broccoli soup. LWT - Food Science and Technology, 111, 167–174. https://doi.org/10.1016/J.LWT.2019.05.037.
Lipton, J. I., Cutler, M., Nigl, F., Cohen, D., & Lipson, H. (2015). Additive manufacturing for the food industry. Trends in Food Science and Technology, 43(1), 114–123. https://doi.org/10.1016/j.tifs.2015.02.004.
Liu, Y., Zhang, W., Wang, K., Bao, Y., Regenstein, J. M., & Zhou, P. (2019). Fabrication of gel-like emulsions with whey protein isolate using microfluidization: Rheological Properties and 3D Printing Performance (Food and Bioprocess Technology, (2019), 12, 12, (1967-1979), 10.1007/s11947-019-02344-5). Food and Bioprocess Technology, 12(12), 1980–1981. https://doi.org/10.1007/s11947-019-02356-1.
Liu, Z., Dick, A., Prakash, S., Bhandari, B., & Zhang, M. (2020). Texture modification of 3D printed air-fried potato snack by varying its internal structure with the potential to reduce oil content. Food and Bioprocess Technology, 13(3), 564–576. https://doi.org/10.1007/s11947-020-02408-x.
Lucas, B. F., de Morais, M. G., Santos, T. D., & Costa, J. A. V. (2018). Spirulina for snack enrichment: nutritional, physical and sensory evaluations. LWT- Food Science and Technology, 90, 270–276. https://doi.org/10.1016/j.lwt.2017.12.032.
Mantihal, S., Prakash, S., Godoi, F. C., & Bhandari, B. (2019). Effect of additives on thermal, rheological and tribological properties of 3D printed dark chocolate. Food Research International, 119(January), 161–169. https://doi.org/10.1016/j.foodres.2019.01.056.
Martínez-Monzó, J., Cárdenas, J., & García-Segovia, P. (2019). Effect of temperature on 3D printing of commercial potato puree. Food Biophysics, 14(3), 1–10. https://doi.org/10.1007/s11483-019-09576-0.
Noort, M. W. J., Van Bommel, K., & Renzetti, S. (2017). 3D-printed cereal foods. Cereal Foods World, 62(November), 272–277. https://doi.org/10.1094/CFW-62-6-0272.
Pallottino, F., Hakola, L., Costa, C., Antonucci, F., Figorilli, S., Seisto, A., & Menesatti, P. (2016). Printing on food or food printing: a review. Food and Bioprocess Technology, 9(5), 725–733. https://doi.org/10.1007/s11947-016-1692-3.
Pedreschi, F., Cortés, P., & Mariotti, M. S. (2018). Potato crisps and snack foods. In Reference Module in Food Science. Elsevier.. https://doi.org/10.1016/B978-0-08-100596-5.21137-2.
Sahni, P., Sharma, S., & Singh, B. (2019). Evaluation and quality assessment of defatted microalgae meal of Chlorella as an alternative food ingredient in cookies. Nutrition and Food Science, 49(2), 221–231. https://doi.org/10.1108/NFS-06-2018-0171.
Schutyser, M. A. I., Houlder, S., de Wit, M., Buijsse, C. A. P., & Alting, A. C. (2018). Fused deposition modelling of sodium caseinate dispersions. Journal of Food Engineering, 220, 49–55. https://doi.org/10.1016/j.jfoodeng.2017.02.004.
Sun, J., Zhou, W., Huang, D., Fuh, J. Y. H., & Hong, G. S. (2015). An overview of 3D printing technologies for food fabrication. Food and Bioprocess Technology, 8(8), 1605–1615. https://doi.org/10.1007/s11947-015-1528-6.
Theagarajan, R., Moses, J. A., & Anandharamakrishnan, C. (2020). 3D extrusion printability of rice starch and optimization of process variables. Food and Bioprocess Technology, 13(6), 1048–1062. https://doi.org/10.1007/s11947-020-02453-6.
Topkaya, C., & Isik, F. (2019). Effects of pomegranate peel supplementation on chemical, physical, and nutritional properties of muffin cakes. Journal of Food Processing and Preservation, (December 2018), 1–11. https://doi.org/10.1111/jfpp.13868.
Ureta, M. M., Olivera, D. F., & Salvadori, V. O. (2014). Quality attributes of muffins: effect of baking operative conditions. Food and Bioprocess Technology, 7(2), 463–470. https://doi.org/10.1007/s11947-012-1047-7.
Uribe-Wandurraga, Z. N., Igual, M., García-Segovia, P., & Martínez-Monzó, J. (2019). Effect of microalgae addition on mineral content, colour and mechanical properties of breadsticks. Food & Function, 10(8), 4685–4692. https://doi.org/10.1039/c9fo00286c.
Uribe-Wandurraga, Z. N., Igual, M., García-Segovia, P., & Martínez-Monzó, J. (2020a). In vitro bioaccessibility of minerals from microalgae-enriched cookies. Food & Function, 11(3), 2186–2194. https://doi.org/10.1039/c9fo02603g.
Uribe-Wandurraga, Z. N., Igual, M., Reino-Moyón, J., García-Segovia, P., & Martínez-Monzó, J. (2020b). Effect of microalgae (Arthrospira platensis and Chlorella vulgaris) addition on 3D printed cookies. Food Biophysics. https://doi.org/10.1007/s11483-020-09642-y.
Vancauwenberghe, V., Verboven, P., Lammertyn, J., & Nicolaï, B. (2018). Development of a coaxial extrusion deposition for 3D printing of customizable pectin-based food simulant. Journal of Food Engineering, 225, 42–52. https://doi.org/10.1016/j.jfoodeng.2018.01.008.
Vieira, M. V., Oliveira, S. M., Amado, I. R., Fasolin, L. H., Vicente, A. A., Pastrana, L. M., & Fuciños, P. (2020). 3D printed functional cookies fortified with Arthrospira platensis: evaluation of its antioxidant potential and physical-chemical characterization. Food Hydrocolloids, 107(March), 105893. https://doi.org/10.1016/j.foodhyd.2020.105893.
Yang, F., Zhang, M., Prakash, S., & Liu, Y. (2018). Physical properties of 3D printed baking dough as affected by different compositions. Innovative Food Science & Emerging Technologies, 49, 202–210. https://doi.org/10.1016/J.IFSET.2018.01.001.
Zhang, L., Lou, Y., & Schutyser, M. A. I. (2018). 3D printing of cereal-based food structures containing probiotics. Food Structure, 18(August), 14–22. https://doi.org/10.1016/j.foostr.2018.10.002.
Zhu, S., Stieger, M. A., van der Goot, A. J., & Schutyser, M. A. I. (2019). Extrusion-based 3D printing of food pastes: correlating rheological properties with printing behaviour. Innovative Food Science & Emerging Technologies, 58(July), 102214. https://doi.org/10.1016/j.ifset.2019.102214.
Acknowledgements
The authors would like to thank Marta Igual from CUINA, Universitat Politècnica de València, for fruitful discussions; and Martin de Wit and Sicong Zhu from FPE, Wageningen University & Research; and Jolanda Henket from WFBR, Wageningen University & Research, for their technical supports.
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Uribe-Wandurraga, Z.N., Zhang, L., Noort, M.W.J. et al. Printability and Physicochemical Properties of Microalgae-Enriched 3D-Printed Snacks. Food Bioprocess Technol 13, 2029–2042 (2020). https://doi.org/10.1007/s11947-020-02544-4
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DOI: https://doi.org/10.1007/s11947-020-02544-4