Abstract
The efficacy of combined UV-C radiation (39.6 J/L) and mild heat (65.0 ± 3.0 °C) on the inactivation of aerobic mesophilic and yeast/molds in grapefruit juice (GFJ) (pH 3.12, °Brix 9.6, 2500 NTU, absorption coefficient 42.9 cm−1) using a flow continuous reactor was investigated. Changes in microbial load, ascorbic acid, and total phenols content, antioxidant capacity, pH, acidity, °Brix, and color were evaluated during 28 days at 4.0 ± 1.0 °C. The spoilage microorganisms were inactivated due to the synergistic effect of both technologies, but the ascorbic acid (74%) and phenol (14%) contents were considerably reduced, with losses of antioxidant capacity (DPPH• = 19% and ABTS•+ = 16%). During storage, the ascorbic acid level and antioxidant capacity gradually decreased. On the other hand, the treated GFJ did not show microbial growth while the untreated GFJ reached more than 4 logs CFU/mL. Conversely, no changes (P < 0.05) in the physicochemical parameters were observed. Principal component analysis is discriminated among the antioxidant compounds and physicochemical properties of the untreated and treated GFJ during storage. Application of UV-C in a Teflon-coil reactor combined with mild heat resulted in longer microbial shelf stability in the GFJ and was able to maintain the physicochemical properties of the juice unchanged during 28 days of storage. Hence, this process can be effectively applied as a combined method to ensure microbial quality.
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Caminiti, I. M., Noci, F., Morgan, D. J., Cronin, D. A., James, G., & Lyng, J. G. (2012). The effect of pulsed electric fields, ultraviolet light or high intensity light pulses in combination with manothermosonication on selected physico-chemical and sensory attributes of an orange and carrot juice blend. Food and Bioprocess Technology, 90(3), 442–448. https://doi.org/10.1016/j.fbp.2011.11.006.
Chen, Y., Yu, L., & Rupasinghe, H. P. V. (2013). Effect of thermal and non-thermal pasteurization on the microbial inactivation and phenolic degradation in fruit juice: a mini-review. Journal of the Science of Food and Agriculture, 93(5), 981–986. https://doi.org/10.1002/jsfa.5989.
Chia, S. L., Rosnah, S., Noranizan, M. A., & Wan Ramli, W. D. (2012). The effect of storage on the quality attributes of ultraviolet-irradiated and thermally pasteurized pineapple juices. International Food Research Journal, 19, 1001–1010.
Del Caro, A., Piga, A., Vacca, V., & Agabbio, M. (2004). Changes of flavonoids, vitamin C and antioxidant capacity in minimally processed citrus segments and juices during storage. Food Chemistry, 84(1), 99–105. https://doi.org/10.1016/S0308-8146(03)00180-8.
Falguera, V., Garza, S., Pagán, J., Garvín, A., & Ibarz, A. (2013). Effect of UV–vis irradiation on enzymatic activities and physicochemical properties of four grape musts from different varieties. Food and Bioprocess Technology, 6(8), 2223–2229. https://doi.org/10.1007/s11947-012-0781-1.
Feng, M., Ghafoor, K., Seo, B., Yang, K., & Park, J. (2013). Effects of ultraviolet-C treatment in Teflon®-coil on microbial populations and physico-chemical characteristics of watermelon juice. Innovative Food Science and Emerging Technologies, 19, 133–139. https://doi.org/10.1016/j.ifset.2013.05.005.
Floegel, A., Kim, D.-O., Chung, S.-J., Koo, S. I., & Chun, O. K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis, 24(7), 1043–1048. https://doi.org/10.1016/j.jfca.2011.01.008.
Franz, C., Specht, I., Cho, G., Graef, V., & Stahl, M. (2009). UV-C-inactivation of microorganisms in naturally cloudy apple juice using novel inactivation equipment based on Dean vortex technology. Food Control, 20(12), 1103–1107. https://doi.org/10.1016/j.foodcont.2009.02.010.
Gautam, D., Umagiliyage, A. L., Dhital, R., Joshi, P., Watson, D. G., Fisher, D. J., & Choudhary, R. (2017). Nonthermal pasteurization of tender coconut water using a continuous flow coiled UV reactor. Lebensmittel-Wissenschaft & Technologie, 83, 127–131. https://doi.org/10.1016/j.lwt.2017.05.008.
Gayán, E., Serrano, M. J., Monfort, S., Álvarez, I., & Condón, S. (2012). Combining ultraviolet light and mild temperatures for the inactivation of Escherichia coli in orange juice. Journal of Food Engineering, 113(4), 598–605. https://doi.org/10.1016/j.jfoodeng.2012.07.018.
Gayán, E., Serrano, J. M., Monfort, S., Álvarez, I., & Condon, S. (2013). Pasteurization of apple juice contaminated with Escherichia coli by a combined UV–mild temperature treatment. Food and Bioprocess Technology, 6(11), 3006–3016. https://doi.org/10.1007/s11947-012-0937-z.
Gayán, E., Serrano, M. J., Pagán, R., Álvarez, I., & Condón, S. (2015). Environmental and biological factors influencing the UV-C resistance of Listeria monocytogenes. Food Microbiology, 46, 246–253. https://doi.org/10.1016/j.fm.2014.08.011.
Guerrero-Beltrán, J. A., & Barbosa-Cánovas, G. V. (2005). Reduction of saccharomyces cerevisiae, escherichia coli and listeria innocua in apple juice by ultraviolet light. Journal of Food Process Engineering, 28(5), 437–452. https://doi.org/10.1111/j.1745-4530.2005.00040.x.
Health Protection Agency (2009). Guidelines for assessing the microbiological safety of ready-to-eat foods. London: Health Protection Agency Available at: http://www.salford.gov.uk/d/Ready_to_Eat_Food_Guidelines_Dec_09_HPA.pdf.
Ibarz, A., Pagán, J., Panadés, R., & Garza, S. (2005). Photochemical destruction of color compounds in fruit juices. Journal of Food Engineering, 69(2), 155–160. https://doi.org/10.1016/j.jfoodeng.2004.08.006.
Igual, M., García-Martínez, E., Camacho, M. M., & Martínez-Navarrete, N. (2010). Effect of thermal treatment and storage on the stability of organic acids and the functional value of grapefruit juice. Food Chemistry, 118(2), 291–299. https://doi.org/10.1016/j.foodchem.2009.04.118.
Kaya, Z., Yıldız, S., & Ünlütürk, S. (2015). Effect of UV-C irradiation and heat treatment on the shelf life stability of a lemon–melon juice blend: multivariate statistical approach. Innovative Food Science and Emerging Technologies, 29, 230–239. https://doi.org/10.1016/j.ifset.2015.03.005.
Koutchma, T., Keller, S., Chirtel, S., & Parisi, B. (2004). Ultraviolet disinfection of juice products in laminar and turbulent flow reactors. Innovative Food Science and Emerging Technologies, 5(2), 179–189. https://doi.org/10.1016/j.ifset.2004.01.004.
Koutchma, T., Popović, V., Ros-Polski, V., & Popielarz, A. (2016). Effects of ultraviolet light and high-pressure processing on quality and health-related constituents of fresh juice products. Comprehensive Reviews in Food Science and Food Safety, 15(5), 844–867. https://doi.org/10.1111/1541-4337.12214.
La Cava, E. L. M., & Sgroppo, S. C. (2015). Evolution during refrigerated storage of bioactive compounds and quality characteristics of grapefruit [Citrus paradisi (Macf.)] juice treated with UV-C light. LWT - Food Science and Technology, 63(2), 1325–1333. https://doi.org/10.1016/j.lwt.2015.04.013.
Lee, H. S., & Coates, G. A. (2006). Thermal pasteurization effects on color of red grapefruit juices. Journal of Food Science, 64(4), 663–666. https://doi.org/10.1111/j.1365-2621.1999.tb15106.x.
Liu, Q., Cedric Tan, C. S., Yang, H., & Wang, S. (2017). Treatment with low-concentration acidic electrolysed water combined with mild heat to sanitise fresh organic broccoli (Brassica oleracea). LWT - Food Science and Technology, 79, 594–600. https://doi.org/10.1016/j.lwt.2016.11.012.
Magalhaes, L. M., Barreiros, L., Maia, M. A., Reis, S., & Segundo, M. A. (2012). Rapid assessment of endpoint antioxidant capacity of red wines through microchemical methods using a kinetic matching approach. Talanta, 97, 473–483. https://doi.org/10.1016/j.talanta.2012.05.002.
Müller, A., Stahl, M. R., Graef, V., Franz, C., & Huch, M. (2011). UV-C treatment of juices to inactivate microorganisms using Dean vortex technology. Journal of Food Engineering, 107(2), 268–275. https://doi.org/10.1016/j.jfoodeng.2011.05.026.
Oteiza, J. M., Peltzer, M., Gannuzzi, L., & Zaritzky, N. (2005). Antimicrobial efficacy of UV radiation on Escherichia coli O157:H7 (EDL 933) in fruit juices of different absorptivities. Journal of Food Protection, 68(1), 49–58. https://doi.org/10.4315/0362-028X-68.1.49.
Pala, Ç. U., & Toklucu, A. K. (2013). Microbial, physicochemical and sensory properties of UV-C processed orange juice and its microbial stability during refrigerated storage. LWT Food Science and Technology, 50(2), 426–431. https://doi.org/10.1016/j.lwt.2012.09.001.
Petin, V. G., Zhurakovskaya, G. P., & Komarova, L. N. (1997). Fluence rate as a determinant of synergistic interaction under simultaneous action of UV light and mild heat in Saccharomyces cerevisiae. Journal of Photochemistry and Photobiology B: Biology, 38(2), 123–128. https://doi.org/10.1016/S1011-1344(96)07449-0.
Rahman, S. M. E., Jin, Y.-G., & Oh, D.-H. (2010). Combined effects of alkaline electrolyzed water and citric acid with mild heat to control microorganisms on cabbage. Journal of Food Science, 75(2), M111–M115. https://doi.org/10.1111/j.1750-3841.2009.01507.x.
Rahn, R. O. (2008). Potassium iodide as a chemical actinometer for 254 nm radiation: use of lodate as an electron scavenger. Photochemistry and Photobiology, 66(4), 450–455. https://doi.org/10.1111/j.1751-1097.1997.tb03172.x.
Scherer, R., Rybka, A. C. P., Ballus, C. A., Meinhart, A. D., Filho, J. T., & Godoy, H. T. (2012). Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices. Food Chemistry, 135(1), 150–154. https://doi.org/10.1016/j.foodchem.2012.03.111.
Sew, C. C., Mohd Ghazali, H., Martín-Belloso, O., & Noranizan, M. A. (2014). Effects of combining ultraviolet and mild heat treatments on enzymatic activities and total phenolic contents in pineapple juice. Innovative Food Science & Emerging Technologies, 26, 511–516. https://doi.org/10.1016/j.ifset.2014.05.008.
Taormina, P. J., & Beuchat, L. R. (2002). Survival of Listeria monocytogenes in commercial food-processing equipment cleaning solutions and subsequent sensitivity to sanitizers and heat. Journal of Applied Microbiology, 92(1), 71–80.
Tikekar, R. V., Anantheswaran, R. C., Elias, R. J., & LaBorde, L. F. (2011). Ultraviolet-induced oxidation of ascorbic acid in a model juice system: identification of degradation products. Journal of Agricultural and Food Chemistry, 59(15), 8244–8248. https://doi.org/10.1021/jf201000x.
Tran, M. T. T., & Farid, M. (2004). Ultraviolet treatment of orange juice. Innovative Food Science and Emerging Technologies, 5(4), 495–502. https://doi.org/10.1016/j.ifset.2004.08.002.
Uckoo, R. M., Jayaprakasha, G. K., Somerville, J. A., Balasubramaniam, V. M., Pinarte, M., & Patil, B. S. (2013). High pressure processing controls microbial growth and minimally alters the levels of health promoting compounds in grapefruit (Citrus paradisi Macfad) juice. Innovative Food Science and Emerging Technologies, 18, 7–14. https://doi.org/10.1016/j.ifset.2012.11.010.
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La Cava, E.L.M., Sgroppo, S.C. Combined Effect of UV-C Light and Mild Heat on Microbial Quality and Antioxidant Capacity of Grapefruit Juice by Flow Continuous Reactor. Food Bioprocess Technol 12, 645–653 (2019). https://doi.org/10.1007/s11947-019-2239-1
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DOI: https://doi.org/10.1007/s11947-019-2239-1