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Ultrasonication of Milky Stage Rice Milk with Bioactive Peptides from Rice Bran: Its Bioactivities and Absorption

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Abstract

Rice in early development stage contains peptides and various bioactive compounds. Rice protein hydrolysate was prepared from Riceberry bran protein hydrolysed with Alcalase® and trypsin. Protein hydrolysate from Alcalase® (MW < 3 kDa) was fractionated into 12 fractions using RP-HPLC and tested for antioxidant, ACE, and ROS-inhibitory activity. Peptide from RP-HPLC fraction 1 showed that the lowest ROS inhibition and highest antioxidant were identified by LC-MS/MS as Val-Leu-Ala-Ala-Gly-Cys-Pro-Leu. Peptide fraction 4 showed that the strongest in vitro ACE-inhibitory activity was identified as Ala-Met-Ser-Phe-Ala-Glu-Met. Young Riceberry milk was then enriched with Alcalase® protein hydrolysate (MW < 3 kDa) and sonicated at amplitude 20% for 30 min and 40% for 60 min. Particle size, transmittance, and Caco-2 cell absorption were measured. The highest peptide content and % absorption was found in young rice milk ultrasonicated at 40% for 60 min. Rice bran protein hydrolysate produced by Alcalase® had much smaller MW bioactive peptides and could be used as a potent functional food ingredient. The young rice milk fortified with bioactive peptides after high power ultra-sonication could increase the in vitro intestinal absorption.

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Funding

This project was partially supported by the National Research Council of Thailand (NRCT), the Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, and the Research and Development Institute, Kasetsart University. Additional thanks to the International Affairs Division, Kasetsart University, for student support.

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Authors and Affiliations

  1. Department of Food Science and Technology, Kasetsart University, Bangkok, 10900, Thailand

    Samuchaya Ngamsuk & Prisana Suwannaporn

  2. Department of Biological Science and Technology, National Pingtung University of Science and Technology, Neipu, Pingtung, 91201, Taiwan

    Jue-Liang Hsu & Tzou-Chi Huang

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  1. Samuchaya Ngamsuk
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  2. Jue-Liang Hsu
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  3. Tzou-Chi Huang
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  4. Prisana Suwannaporn
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Correspondence to Prisana Suwannaporn.

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Ngamsuk, S., Hsu, JL., Huang, TC. et al. Ultrasonication of Milky Stage Rice Milk with Bioactive Peptides from Rice Bran: Its Bioactivities and Absorption. Food Bioprocess Technol 13, 462–474 (2020). https://doi.org/10.1007/s11947-019-02371-2

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  • DOI: https://doi.org/10.1007/s11947-019-02371-2

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