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Combination Effect of High-Pressure Processing and Essential Oil (Melissa officinalis Extracts) or Their Constituents for the Inactivation of Escherichia coli in Ground Beef

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Abstract

The inactivation of a five-strain cocktail of Escherichia coli by high-pressure processing (HPP) and Melissa officinalis leaf extracts (MoEOs) or their similar chemical constituents (CCs; citral, geraniol, β-caryophyllene or a mixture) in fresh ground beef was investigated. The pathogenic bacteria post-process growth and survival were further determined at 4 °C, 7 days storage. Ground beef pressurized at 300, 350, and 400 MPa for 15 min in combination with 0.5 and 1.0% of MoEOs or CCs was investigated. A 5-log CFU/g reduction was achieved with properly selected pressure and MoEOs, CCs, or MIX [a reconstituted mixture of 1/3 citral, 1/3 geraniol, and 1/3 β-caryophyllene (weight basis)] concentration. Without the HPP, the inactivation potential of citral, geraniol, MoEOs, or MIX was similar and negligible, while under pressure, the inactivation potential increased significantly as the pressure and concentration increased. The Escherichia coli strains tested include Shiga toxin-producing E. coli (STEC) O157, O111, O121, O128, and O145, which are involved in many food-borne pathogen outbreaks worldwide. For 24 h under refrigeration, 1.0% citral, 1.0% geraniol, 1.0% MIX, and 1.0% MoEOs with 350 and 400 MPa could reduce ca. 3–6 log CFU/g of E. coli. The inactivation potential continued to show effectiveness during the low-temperature storage test (e.g., 4 °C, 7 days). In conclusion, the combination treatment of HPP and M. officinalis extracts was found to be significantly effective in the inactivation of E. coli. Transmission electron microscope (TEM) images further demonstrated the cell structure damaged under HPP and antimicrobial compound stresses.

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Acknowledgments

This research was supported by the USDA Agricultural Research Service National Program 108 (Food Safety) Project # 1935-42000-078-00D.

The Natural Products Innovation Laboratories located in Chiayi Industry Innovation and Research Center (Chiayi, Taiwan) is acknowledged for providing equipment/facility to prepare essential oil extracts.

We also would like to thank Dr. Alberto Nuñez for his generous assistance in analyzing the components of essential oil extracts with GC-MS and Mr. Joseph Uknalis for his delicate work on the electron microscopy images (TEM). Both researchers are with the USDA/ARS/ERRC.

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  1. Institute of Food Science and Technology, The National Taiwan University, Taipei, Taiwan

    Shih-Yung Chien & Lee-Yan Sheen

  2. Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA, 19038, USA

    Shih-Yung Chien, Shiowshuh Sheen & Christopher Sommers

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Chien, SY., Sheen, S., Sommers, C. et al. Combination Effect of High-Pressure Processing and Essential Oil (Melissa officinalis Extracts) or Their Constituents for the Inactivation of Escherichia coli in Ground Beef. Food Bioprocess Technol 12, 359–370 (2019). https://doi.org/10.1007/s11947-018-2211-5

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