Food and Bioprocess Technology

, Volume 11, Issue 5, pp 1050–1060 | Cite as

Construction of a Biocompatible and Antioxidant Multilayer Coating by Layer-by-Layer Assembly of κ-Carrageenan and Quercetin Nanoparticles

  • Marthyna P. Souza
  • Antônio F. M. Vaz
  • Thacianna B. Costa
  • Miguel A. Cerqueira
  • Célia M. M. B. De Castro
  • António A. Vicente
  • Maria G. Carneiro-da-Cunha
Original Paper


The present work aimed at the construction and characterization of a multilayer coating based on κ-carrageenan and quercetin-loaded lecithin/chitosan nanoparticles (Np) by the layer-by-layer technique and the evaluation of its antioxidant capacity and potential cytotoxicity in vitro. The multilayered coating was successfully self-assembled, as confirmed by UV-Vis spectroscopy, contact angle, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Multilayered coatings showed to have antioxidant capacity, with a DPPH• radical scavenging activity of 31.32 ± 3.13% and a result of the FRAP assay of 799.41 ± 95.39 μM of ferrous ion (Fe2+) equivalent. These coatings were also shown to be devoid of cell toxicity, as evaluated by determination of nitric oxide production and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test. The alveolar macrophages culture was tested in the presence of the κ-carrageenan/quercetin-Np multilayer coating and showed a cell viability of 91.3 ± 9.6%. These results suggest that this multilayered coating is adequate for surfaces modification in view of biomedical and food industry applications.


Biomaterial Cytotoxicity Multilayer Polyelectrolyte Surface modification 



This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. The authors would also like to thank the Brazilian Government for support given by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Carneiro-da-Cunha, M.G. expresses her gratitude to the CNPq for research grant.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Marthyna P. Souza
    • 1
    • 2
    • 3
  • Antônio F. M. Vaz
    • 4
  • Thacianna B. Costa
    • 2
  • Miguel A. Cerqueira
    • 3
    • 5
  • Célia M. M. B. De Castro
    • 2
  • António A. Vicente
    • 3
  • Maria G. Carneiro-da-Cunha
    • 1
    • 2
  1. 1.Biochemistry DepartmentUniversidade Federal de Pernambuco-UFPERecifeBrazil
  2. 2.LIKA - Laboratório de Imunopatologia Keizo AsamiUFPE, Cidade UniversitariaRecifeBrazil
  3. 3.CEB – Centre of Biological EngineeringUniversidade do MinhoBragaPortugal
  4. 4.Unidade Acadêmica de Medicina VeterináriaUniversidade Federal de Campina GrandePatosBrazil
  5. 5.International Iberian Nanotechnology Laboratory-INLBragaPortugal

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