Abstract
Combinations of essential oils (EOs) can be an effective approach to reinforce their antimicrobial effects. In this sense, incorporation of two EOs into edible films may have supplementary utilizations in food packaging. Chitosan films containing combined EOs of cinnamon and ginger (1:1) at levels of 0.00, 0.05, 0.20, and 1.00% were developed and preliminarily characterized in the current study. The effect of the resulting materials on the antimicrobial and antioxidant properties of pork was then investigated during refrigerated storage (4 °C) over 9 days. Results showed that the presence of EOs markedly increased the thickness and opacity of the chitosan films, but did not modify the film solubility and water vapor permeability. When applied to the preservation of pork slices, these films were effective in retarding total microbial growth, increases in pH as well as lipid oxidation. The highest antioxidant and antimicrobial activities were observed in chitosan films incorporated with 1.00% EOs. These results suggest that chitosan-EO films have potential for application in pork packaging.






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Abdollahi, M., Rezaei, M., & Farzi, G. (2012). Improvement of active chitosan film properties with rosemary essential oil for food packaging. International Journal of Food Science & Technology, 47(4), 847–853.
Aider, M. (2010). Chitosan application for active bio-based films production and potential in the food industry: review. LWT - Food Science and Technology, 43(6), 837–842.
Amaral, D. S., Cardelle-Cobas, A., Nascimento, B. M., Monteiro, M. J., Madruga, M. S., & Pintado, M. M. (2015). Development of a low fat fresh pork sausage based on chitosan with health claims: impact on the quality, functionality and shelf-life. Food & Function, 6(8), 2768–2778.
Amaral, D. S., Cardelle-Cobas, A., Nascimento, B. M., Madruga, M. S., & Pintado, M. M. (2016). Goat sausages containing chitosan towards a healthier product: microbiological, physico-chemical textural evaluation. Food & Function, 7(9), 4020–4029.
ASTM. (1995). Standard test methods for water vapor transmission of material, E96–95. Philadelphia: American Society for Testing and Material.
ASTM. (1997). Standard test method for transparency of plastic sheeting, D1746. West Conshohocken: ASTM International.
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils—a review. Food and Chemical Toxicology, 46(2), 446–475.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223–253.
Donsì, F., Annunziata, M., Sessa, M., & Ferrari, G. (2011). Nanoencapsulation of essential oils to enhance their antimicrobial activity in foods. LWT - Food Science and Technology, 44(9), 1908–1914.
Du, H., & Li, H. (2008). Antioxidant effect of Cassia essential oil on deep-fried beef during the frying process. Meat Science, 78(4), 461–468.
Fasseas, M. K., Mountzouris, K. C., Tarantilis, P. A., Polissiou, M., & Zervas, G. (2008). Antioxidant activity in meat treated with oregano and sage essential oils. Food Chemistry, 106(3), 1188–1194.
Georgantelis, D., Ambrosiadis, I., Katikou, P., Blekas, G., & Georgakis, S. A. (2007). Effect of rosemary extract, chitosan and α-tocopherol on microbiological parameters and lipid oxidation of fresh pork sausages stored at 4 °C. Meat Science, 76(1), 172–181.
Gómez-Estaca, J., Montero, P., Fernández-Martín, F., & Gómez-Guillén, M. C. (2009). Physico-chemical and film-forming properties of bovine-hide and tuna-skin gelatin: a comparative study. Journal of Food Engineering, 90(4), 480–486.
Hafsa, J., Smach, M. A., Khedher, M. R. B., Charfeddine, B., Limem, K., Majdoub, H., et al. (2015). Physical, antioxidant and antimicrobial properties of chitosan films containing Eucalyptus globulus essential oil. LWT - Food Science and Technology, 68, 356–364.
He, S., Yang, Q., Ren, X., Zi, J., Lu, S., Wang, S., et al. (2014). Antimicrobial efficiency of chitosan solutions and coatings incorporated with clove oil and/or ethylenediaminetetraacetate. Journal of Food Safety, 34(4), 345–352.
He, S., Wang, Y., Sun, Y., Chen, S., Zhang, Y., & Ying, M. (2015). Antimicrobial activity and preliminary characterization of κ-carrageenan films containing cinnamon essential oil. Advance Journal of Food Science and Technology, 9(7), 523–528.
Hettiarachchy, N. S., Glenn, K. C., Gnanasambandam, R., & Johnson, M. G. (1996). Natural antioxidant extract from Fenugreek (Trigonella foenumgraecum) for ground beef patties. Journal of Food Science, 61(3), 516–519.
Holley, R. A., & Patel, D. (2005). Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiology, 22(4), 273–292.
Hosseini, M. H., Razavi, S. H., & Mousavi, M. A. (2009). Antimicrobial, physical and mechanical properties of chitosan-based films incorporated with thyme, clove and cinnamon essential oils. Journal of Food Processing and Preservation, 33(6), 727–743.
Hu, J., Wang, X., Xiao, Z., & Bi, W. (2015). Effect of chitosan nanoparticles loaded with cinnamon essential oil on the quality of chilled pork. LWT - Food Science and Technology, 63(1), 519–526.
Huff-Lonergan, E., & Lonergan, S. M. (2005). Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes. Meat Science, 71(1), 194–204.
Lahucky, R., Nuernberg, K., Kovac, L., Bucko, O., & Nuernberg, G. (2010). Assessment of the antioxidant potential of selected plant extracts—in vitro and in vivo experiments on pork. Meat Science, 85(4), 779–784.
Lamas, A., Anton, X., Miranda, J. M., Roca-Saavedra, P., Cardelle-Cobas, A., Ibarra, I. S., et al. (2016). Technological strategies for the development of egg-derived products with reduced content of cholesterol. Food and Bioprocess Technology, 9(1), 81–90.
Ma, Q., Zhang, Y., & Zhong, Q. (2016). Physical and antimicrobial properties of chitosan films incorporated with lauric arginate, cinnamon oil, and ethylenediaminetetraacetate. LWT - Food Science and Technology, 65, 173–179.
Miao, J., Peng, W., Liu, G., Chen, Y., Chen, F., & Cao, Y. (2015). Biopreservative effect of the natural antimicrobial substance from Lactobacillus paracasei subsp. tolerans FX-6 on fresh pork during chilled storage. Food Control, 56, 53–56.
Murphy, M. M., Spungen, J. H., Bi, X., & Barraj, L. M. (2011). Fresh and fresh lean pork are substantial sources of key nutrients when these products are consumed by adults in the United States. Nutrition Research, 31(10), 776–783.
Ojagh, S. M., Rezaei, M., Razavi, S. H., & Hosseini, S. M. H. (2010). Development and evaluation of a novel biodegradable film made from chitosan and cinnamon essential oil with low affinity toward water. Food Chemistry, 122(1), 161–166.
Pearce, K. L., Rosenvold, K., Andersen, H. J., & Hopkins, D. L. (2011). Water distribution and mobility in meat during the conversion of muscle to meat and ageing and the impacts on fresh meat quality attributes—a review. Meat Science, 89(2), 111–124.
Peng, Y., & Li, Y. (2014). Combined effects of two kinds of essential oils on physical, mechanical and structural properties of chitosan films. Food Hydrocolloids, 36, 287–293.
Pérez-Rosés, R., Risco, E., Vila, R., Peñalver, P., & Cañigueral, S. (2016). Biological and nonbiological antioxidant activity of some essential oils. Journal of Agricultural and Food Chemistry, 64(23), 4716–4724.
Pires, C., Ramos, C., Teixeira, G., Batista, I., Mendes, R., Nunes, L., et al. (2011). Characterization of biodegradable films prepared with hake proteins and thyme oil. Journal of Food Engineering, 105(3), 422–428.
Remya, S., Mohan, C. O., Bindu, J., Sivaraman, G. K., Venkateshwarlu, G., & Ravishankar, C. N. (2016). Effect of chitosan based active packaging film on the keeping quality of chilled stored barracuda fish. Journal of Food Science and Technology, 53(1), 685–693.
Salgado, P. R., López-Caballero, M. E., Gómez-Guillén, M. C., Mauri, A. N., & Montero, M. P. (2013). Sunflower protein films incorporated with clove essential oil have potential application for the preservation of fish patties. Food Hydrocolloids, 33(1), 74–84.
Sathivel, S., Liu, Q., Huang, J., & Prinyawiwatkul, W. (2007). The influence of chitosan glazing on the quality of skinless pink salmon (Oncorhynchus gorbuscha) fillets during frozen storage. Journal of Food Engineering, 83(3), 366–373.
Sayas-Barberá, E., Quesada, J., Sánchezzapata, E., Viudamartos, M., Fernándezlópez, F., Pérezalvarez, J. A., et al. (2011). Effect of the molecular weight and concentration of chitosan in pork model burgers. Meat Science, 88(4), 740–749.
Shahidi, F., Arachchi, J. K. V., & Jeon, Y. J. (1999). Food applications of chitin and chitosans. Trends in Food Science & Technology, 10(2), 37–51.
Soultos, N., Tzikas, Z., Abrahim, A., Georgantelis, D., & Ambrosiadis, I. (2008). Chitosan effects on quality properties of Greek style fresh pork sausages. Meat Science, 80(4), 1150–1156.
Yuan, G., Chen, X., & Li, D. (2016). Chitosan films and coatings containing essential oils: the antioxidant and antimicrobial activity, and application in food systems. Food Research International, 89, 117–128.
Zhang, W. G., Lonergan, S. M., Gardner, M. A., & Huff-Lonergan, E. (2006). Contribution of postmortem changes of integrin, desmin and μ-calpain to variation in water holding capacity of pork. Meat Science, 74(3), 578–585.
Zinoviadou, K. G., Koutsoumanis, K. P., & Biliaderis, C. G. (2009). Physico-chemical properties of whey protein isolate films containing oregano oil and their antimicrobial action against spoilage flora of fresh beef. Meat Science, 82(3), 338–345.
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This work was supported by alliance plan (LM201644) of SPAT.
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Wang, Y., Xia, Y., Zhang, P. et al. Physical Characterization and Pork Packaging Application of Chitosan Films Incorporated with Combined Essential Oils of Cinnamon and Ginger. Food Bioprocess Technol 10, 503–511 (2017). https://doi.org/10.1007/s11947-016-1833-8
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DOI: https://doi.org/10.1007/s11947-016-1833-8

