In this study, we describe the extraction of different valuable compounds from winter melon seeds using supercritical carbon dioxide extraction combined with pressure swing technique (SCE-PST). The effects of the extraction variables, namely pressure, holding time (HT), and continuous extraction time (CT), were optimized by response surface methodology (RSM) to maximize the crude extraction yield (CEY). The optimal conditions were at pressure of 181.35 bar, HT of 9.93 min, and CT of 50.14 min. Under these conditions, the experimental CEY was 235.70 ± 0.11 mg g−1 with a relatively strong antioxidant activity (64.42 ± 0.21 % inhibition of DPPH· radicals, 67.36 ± 0.34 % inhibition of ABTS·+ radicals) and considerable amount of phenolic compounds (42.77 ± 0.40 mg gallic acid equivalent/g extract). The high-performance liquid chromatography (HPLC) analysis revealed that the bioactive phenolic compounds increased significantly using PST (p < 0.05), where gallic acid had the highest concentration (0.688 ± 0.34 mg g−1). The extract obtained using optimal SCE-PST conditions contained more than 83.65 % total unsaturated fatty acids (UFAs) and linoleic acid accounted for 67.33 ± 0.22 % in the total extract. From the results, the SCE efficiency in terms of extract quantity and quality has been enhanced significantly applying PST. Finally, the results were compared with previous published findings using supercritical carbon dioxide, ultrasound-assisted, and Soxhlet extraction. It was found that higher CEY could be achieved using Soxhlet extraction even through the quality of SCE-PST extracts in terms of antioxidant activity and phenolic compounds was better.
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Al-Khalifa, A. S. (1996). Physicochemical characteristics, fatty acid composition, and lipoxygenase activity of crude pumpkin and melon seed oils. Journal of Agricultural and Food Chemistry, 44, 964–966.
Al-Naqeeb, G., Ismail, M., & Al-Zubairi, A. S. (2009). Fatty acid profile, α-tocopherol content and total antioxidant activity of oil extracted from Nigella sativa seeds. International Journal of Pharmacology, 5, 244–250.
Bas, D., & Boyaci, I. H. (2007). Modeling and optimization I, usability of response surface methodology. Journal of Food Engineering, 78, 836–845.
Bhattacharjee, P., Singhal, R. S., & Tiwari, S. R. (2007). Supercritical carbon dioxide extraction of cottonseed oil. Journal of Food Engineering, 79, 892–898.
Bimakr, M., Rahman, R. A., Taip, F. S., Adzahan, N. M., Sarker, M. Z. I., & Ganjloo, A. (2012). Optimization of ultrasound-assisted extraction of crude oil from winter melon (Benincasa hispida) seed using response surface methodology and evaluation of its antioxidant activity, total phenolic content and fatty acid composition. Molecules, 7, 11748–11762.
Bimakr, M., Rahman, R. A., Taip, F. S., Adzahan, N. M., Sarker, M. Z. I., & Ganjloo, A. (2013). Supercritical carbon dioxide extraction of seed oil from winter melon (Benincasa hispida) and its antioxidant activity and fatty acid composition. Molecules, 18, 997–1014.
Cao, X., & Ito, Y. (2003). Supercritical fluid extraction of grape seed oil and subsequent separation of free fatty acids by high-speed counter-current chromatography. Journal of Chromatography A, 1021, 117–124.
Ensminger, M. E., Oldfield, J. E., & Heinemann, W. W. (1990). Feeds and nutrition. 2nd edition. Ensminger, Clovis
Kazzazi, H., Rezaei, K., Ghotb-Sharif, S. J., Emam-Djomeh, Z., & Yamini, Y. (2007). Supercritical fluid extraction of flavors and fragrances from Hyssopus officinalis L. cultivated in Iran. Food Chemistry, 105, 805–811.
Khan, M. K., Abert-Vian, M., Fabiano-Tixier, A. S., Dangles, O., & Chemat, F. (2010). Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry, 119, 851–858.
Lee, W. Y., Cho, Y. J., Oh, S. L., Park, J. H., Cha, W. S., Jung, J. Y., & Choi, Y. H. (2000). Extraction of grape seed oil by supercritical CO2 and ethanol modifier. Food Science and Biotechnology, 9, 174–178.
Liu, G., Xu, X., Hao, Q., & Gao, Y. (2009). Supercritical CO2 extraction optimization of pomegranate (Punica granatum L.) seed oil using response surface methodology. LWT--Food Science and Technology, 42, 1491–1495.
Liyana-Pathirana, C., & Shahidi, F. (2005). Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chemistry, 93, 47–56.
Liza, M. S., Abdul Rahman, R., Mandana, B., Jinap, S., Rahmat, A., Zaidul, I. S. M., & Hamid, S. (2010). Supercritical carbon dioxide extraction of bioactive flavonoid from Strobilanthes crispus (Pecah Kaca). Food and Bioproducts Processing, 88, 319–326.
Luengthanaphol, S., Mongkholkhajornsilp, D., Douglas, S., Douglas, P. L., Pengsopa, L., & Pongamphai, S. (2004). Extraction of antioxidants from sweet Thai tamarind seed coat—preliminary experiments. Journal of Food Engineering, 63, 247–252.
Machmudah, S., Kawahito, Y., Sasaki, M., & Goto, M. (2007). Supercritical CO2 extraction of rosehip seed oil: fatty acids composition and process optimization. Journal of Supercritical Fluids, 41, 421–428.
Mandana, B., Russly, A. R., Farah, S. T., Noranizan, M. A., Zaidul, I. S., & Ali, G. (2012). Antioxidant activity of winter melon (Benincasa hispida) seeds using conventional Soxhlet extraction technique. International Food Research Journal, 19, 229–234.
Mariod, A. A., Ahmed, Y. M., Matthäus, B., Khaleel, G., Siddig, A., Gabra, A. M., & Abdelwahab, S. I. (2009). A comparative study of the properties of six Sudanese cucurbit seeds and seed oils. Journal of the American Oil Chemists' Society, 86, 1181–1188.
Martinez, J. L. (2008). Supercritical fluid extraction of nutraceuticals and bioactive compounds. New York: United States of America, CRC Press.
Mingyu, D., Mingzhang, L., Qinghong, Y., Weiming, F., Jianxiang, X., & Weiming, X. (1995). A study on Benincasa hispida contents effective for protection of kidney. Jiangsu Journal of Agricultural Sciences, 11, 46–52.
Mirhosseini, H., Tan, C. P., Hamid, N. S. A., & Yusof, S. (2008). Effect of Arabic gum, xanthan gum and orange oil on flavour release from diluted orange beverage emulsion. Food Chemistry, 107, 1161–1172.
Montgomery, D. C. (2001). Design and analysis of experiments (5th ed.). New York: Wiley.
Nyam, K. L., Tan, C. P., Lai, O. M., Long, K., & Che Man, Y. B. (2009). Physicochemical properties and bioactive compounds of selected seed oils. LWT - Food Science and Technology, 42, 1396–1403.
Oliveira, R., Rodrigues, M. F., & Bernardo-Gil, M. G. (2002). Characterization and supercritical carbon dioxide extraction of walnut oil. Journal of the American Oil Chemists' Society, 79, 225–230.
Rezaei, K., & Temelli, F. (2000). Using supercritical fluid chromatography to determine diffusion coefficients of lipids in supercritical CO2. Journal of Supercritical Fluids, 17, 35–44.
Rezzoug, S. A., Boutekedjiret, C., & Allaf, K. (2005). Optimization of operating conditions of rosemary essential oil extraction by a fast controlled pressure drop process using response surface methodology. Journal of Food Engineering, 71, 9–17.
Salto, S. (1995). Research activities on supercritical fluid science and technology in Japan—a review. Journal of Supercritical Fluids, 8, 177–204.
Sánchez-Vicente, Y., Cabañas, A., Renuncio, J. A. R., & Pando, C. (2009). Supercritical fluid extraction of peach (Prunus persica) seed oil using carbon dioxide and ethanol. Journal of Supercritical Fluids, 49, 167–173.
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178.
Smith, R. L., Jr., Malaluan, R. M., Setianto, W. B., Inomata, H., & Arai, K. (2003). Separation of cashew (Anacardium occidentale L.) nut shell liquid with supercritical carbon dioxide. Bioresource Technology, 88, 1–7.
Stévigny, C., Rolle, L., Valentini, N., & Zeppa, G. (2007). Optimization of extraction of phenolic content from hazelnut shell using response surface methodology. Journal of the Science of Food and Agriculture, 87, 2817–2822.
Thana, P., Machmudah, S., Goto, M., Sasaki, M., Pavasant, P., & Shotipruk, A. (2008). Response surface methodology to supercritical carbon dioxide extraction of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 99, 3110–3115.
Triveni, R., Shamala, T. R., & Rastogi, N. K. (2001). Optimised production and utilisation of exopolysaccharide from Agrobacterium radiobacter. Process Biochemistry, 36, 787–795.
Valcárcel, M., & Tena, M. T. (1997). Applications of supercritical fluid extraction in food analysis. Fresenius Journal of Analytical Chemistry, 358, 561–573.
Wang, L., & Weller, C. L. (2006). Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology, 17, 300–312.
Wang, L., Yang, B., Du, X., & Yi, C. (2008). Optimisation of supercritical fluid extraction of flavonoids from Pueraria lobata. Food Chemistry, 108, 737–741.
Wei, Z. J., Liao, M. A., Zhang, H. X., Liu, J., & Jiang, S. H. (2009). Optimization of supercritical carbon dioxide extraction of silkworm pupal oil applying the response surface methodology. Bioresource Technology, 100, 4214–4219.
Wilkinson, P., Leach, C., Ahsing, E. E., Hussain, N., Miller, G. J., & Millward, D. J. (2005). Influence of α-linolenic acid and fish-oil on markers of cardiovascular risk in subjects with an atherogenic lipoprotein phenotype. Atherosclerosis, 181, 115–124.
Zaidul, I. S. M., Norulaini, N. A. N., Omar, A. K. M., Sato, Y., & Smith, R. L., Jr. (2007). Separation of palm kernel oil from palm kernel with supercritical carbon dioxide using pressure swing technique. Journal of Food Engineering, 81, 419–428.
Zaini, N. A. M., Anwar, F., Hamid, A. A., & Saari, N. (2011). Kundur [Benincasa hispida (Thunb.) Cogn.]: a potential source for valuable nutrients and functional foods. Food Research International, 44, 2368–2376.
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Bimakr, M., Rahman, R.A., Ganjloo, A. et al. Characterization of Valuable Compounds from Winter Melon (Benincasa hispida (Thunb.) Cogn.) Seeds Using Supercritical Carbon Dioxide Extraction Combined with Pressure Swing Technique. Food Bioprocess Technol 9, 396–406 (2016). https://doi.org/10.1007/s11947-015-1636-3