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Characterization of Valuable Compounds from Winter Melon (Benincasa hispida (Thunb.) Cogn.) Seeds Using Supercritical Carbon Dioxide Extraction Combined with Pressure Swing Technique

Food and Bioprocess Technology volume 9, pages 396–406 (2016)Cite this article

Abstract

In this study, we describe the extraction of different valuable compounds from winter melon seeds using supercritical carbon dioxide extraction combined with pressure swing technique (SCE-PST). The effects of the extraction variables, namely pressure, holding time (HT), and continuous extraction time (CT), were optimized by response surface methodology (RSM) to maximize the crude extraction yield (CEY). The optimal conditions were at pressure of 181.35 bar, HT of 9.93 min, and CT of 50.14 min. Under these conditions, the experimental CEY was 235.70 ± 0.11 mg g−1 with a relatively strong antioxidant activity (64.42 ± 0.21 % inhibition of DPPH· radicals, 67.36 ± 0.34 % inhibition of ABTS·+ radicals) and considerable amount of phenolic compounds (42.77 ± 0.40 mg gallic acid equivalent/g extract). The high-performance liquid chromatography (HPLC) analysis revealed that the bioactive phenolic compounds increased significantly using PST (p < 0.05), where gallic acid had the highest concentration (0.688 ± 0.34 mg g−1). The extract obtained using optimal SCE-PST conditions contained more than 83.65 % total unsaturated fatty acids (UFAs) and linoleic acid accounted for 67.33 ± 0.22 % in the total extract. From the results, the SCE efficiency in terms of extract quantity and quality has been enhanced significantly applying PST. Finally, the results were compared with previous published findings using supercritical carbon dioxide, ultrasound-assisted, and Soxhlet extraction. It was found that higher CEY could be achieved using Soxhlet extraction even through the quality of SCE-PST extracts in terms of antioxidant activity and phenolic compounds was better.

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Authors and Affiliations

  1. Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mandana Bimakr, Russly Abdul Rahman & Noranizan Mohd Adzahan

  2. Halal Product Research Institute, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia

    Russly Abdul Rahman

  3. Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, 43400, Selangor, Malaysia

    Russly Abdul Rahman & Farah Saleena Taip

  4. Department of Food Science and Technology, Faculty of Agriculture, University of Zanjan, Zanjan, 45371-38791, Iran

    Ali Ganjloo

  5. Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic Universiti Malaysia, 25200, Kuntan, Pahang, Malaysia

    Md Zaidul Islam Sarker

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  1. Mandana Bimakr
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  2. Russly Abdul Rahman
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  4. Farah Saleena Taip
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  5. Noranizan Mohd Adzahan
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  6. Md Zaidul Islam Sarker
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Correspondence to Mandana Bimakr.

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Bimakr, M., Rahman, R.A., Ganjloo, A. et al. Characterization of Valuable Compounds from Winter Melon (Benincasa hispida (Thunb.) Cogn.) Seeds Using Supercritical Carbon Dioxide Extraction Combined with Pressure Swing Technique. Food Bioprocess Technol 9, 396–406 (2016). https://doi.org/10.1007/s11947-015-1636-3

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