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Morphology and Stability of Edible Lycopene-Containing Micro- and Nanocapsules Produced Through Electrospraying and Spray Drying

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In this work, lycopene was encapsulated through electrospraying and spray drying (using a microporous membrane cap) within different edible biopolymeric matrices. Specifically, dextran, a whey protein concentrate (WPC) and chitosan were used as matrix materials. As a strategy to incorporate the hydrophobic bioactive within the hydrophilic matrices, emulsion electrospraying and spray drying from emulsion were carried out. Moreover and for comparison purposes, coaxial electrospraying was also performed. The electrospraying solutions properties were studied, since they do not only affect the success of the electrohydrodynamic process but also influence the morphology of the capsules. Apart from characterizing the morphology and molecular organization of the developed capsules, the encapsulation efficiency and the lycopene stability under moisture and heating conditions were also evaluated. Results showed that even though encapsulation structures were obtained from all the matrices assayed through both processing technologies, spray drying, as a consequence of the high temperatures needed in this process, affected lycopene stability and very poor encapsulation efficiencies were found in this case. It was also seen that WPC presented the greatest encapsulation efficiency (around 75 %), probably ascribed to the interactions between the biopolymer and the lycopene. Furthermore, WPC capsules were able to better protect lycopene against moisture and thermal degradation.

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  • Angeles, M., Cheng, H.-L., & Velankar, S. S. (2008). Emulsion electrospinning: composite fibres from drop breakup during electrospinning. Polymers for Advanced Technologies, 19, 728–733.

    Article  CAS  Google Scholar 

  • Blanch, G. P., Castillo, M. L. R., Caja, M. M., Pérez-Méndez, M., & Sánchez-Cortés, S. (2007). Stabilization of all-trans-lycopene from tomato by encapsulation using cyclodextrins. Food Chemistry, 105, 1335–1341.

    Article  CAS  Google Scholar 

  • Demiray, E., Tulek, Y., & Yilmaz, Y. (2013). Degradation kinetics of lycopene, β-carotene and ascorbic acid in tomatoes during hot air drying. LWT - Food Science and Technology, 50(1), 172–176.

    Article  CAS  Google Scholar 

  • Eissa, A. S., Puhl, C., Kadla, J. F., & Khan, S. A. (2006). Enzymatic cross-linking of β-lactoglobulin: conformational properties using FTIR spectroscopy. Biomacromolecules, 7(6), 1707–1713.

    Article  CAS  Google Scholar 

  • Fernandez, A., Torres-Giner, S., & Lagaron, J. M. (2009). Novel route to stabilization of bioactive antioxidants by encapsulation in electrospun fibres of zein prolamine. Food Hydrocolloids, 23(5), 1727–1432.

    Article  Google Scholar 

  • Fernandez-Saiz, P., Ocio, M. J., & Lagaron, J. M. (2006). Film-forming process and biocide assessment of high-molecular-weight chitosan as determined by combined ATR-FTIR spectroscopy and antimicrobial assays. Biopolymers, 83, 577–583.

    Article  CAS  Google Scholar 

  • Goula, A. M., & Adamopoulos, K. G. (2005). Stability of lycopene during spray drying of tomato pulp. LWT - Food Science and Technology, 38(5), 479–487.

    Article  CAS  Google Scholar 

  • Guo, H., Huang, Y., Qian, J.-q., Gong, Q.-y., & Tang, Y. (2012). Optimization of technological parameters for preparation of lycopene microcapsules. Journal of Food Science and Technology. doi:10.1007/s13197-011-0600-0.

    Google Scholar 

  • Hong, Y. L., Li, Y. Y., Yin, Y. Z., Li, D. M., & Zhou, G. T. (2008). Electro-hydrodynamic atomization of quasi-monodisperse drug-loaded spherical/wrinkled microparticles. Journal of Aerosol Science, 39(6), 525–536.

    Article  CAS  Google Scholar 

  • Kong, J., & Yu, S. (2007). Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta Biochimica et Biophysica Sinica, 39(8), 549–559.

    Article  CAS  Google Scholar 

  • Li, D., & Xia, Y. (2004). Electrospinning of nanofibres: reinventing the wheel? Advanced Materials, 16(14), 1151–1170.

    Article  CAS  Google Scholar 

  • Matioli, G., & Rodriguez-Amaya, D. B. (2002). Licopeno encapsulado em goma arábica e maltodextrina: estudo da estabilidade. Brazilian Journal of Food Technology, 5, 197–203.

    Google Scholar 

  • Mensi, A., Choiset, Y., Rabesona, H., Haertlé, T., Borel, P., & Chobert, J.-M. (2013). Interactions of β-lactoglobulin variants A and B with vitamin A. Competitive binding of retinoids and carotenoids. Journal of Agricultural and Food Chemistry, 6(17), 4114–4119.

    Article  Google Scholar 

  • Pérez-Masiá, R., Fabra, M.J., Lagarón, J.M., López-Rubio, A. (2013). Use of electrospinning for encapsulation. In: Vikas Mittal (Ed.), Encapsulation nanotechnologies, pp. 107–136. Scrivener & Wiley, Abu Dhabi.

  • Pérez-Masiá, R., Lagarón, J. M., & López-Rubio, A. (2014). Development and optimization of novel encapsulation structures of interest in functional foods through electrospraying. Food and Bioprocess Technology. doi:10.1007/s11947-014-1304-z.

    Google Scholar 

  • Pillai, C. K. S., Paul, W., & Sharma, C. P. (2009). Chitin and chitosan polymers: chemistry, solubility and fibre formation. Progress in Polymer Science (Oxford), 34(7), 641–678.

    Article  CAS  Google Scholar 

  • Rao, A. V., & Agarwal, S. (1999). Role of lycopene as antioxidant carotenoid in the prevention of chronic disease: a review. Nutrition Research, 19, 305–323.

    Article  CAS  Google Scholar 

  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237.

    Article  CAS  Google Scholar 

  • Rocha, G. A., Fávaro-Trindade, C. S., & Grosso, C. R. F. (2012). Microencapsulation of lycopene by spray drying: characterization, stability and application of microcapsules. Food and Bioproducts Processing, 90, 37–42.

    Article  Google Scholar 

  • Rocha-Selmi, G. A., Favaro-Trindade, C. S., & Grosso, C. R. F. (2013). Morphology, stability, and application of lycopene microcapsules produced by complex coacervation. Journal of Chemistry. doi:10.1155/2013/982603.

    Google Scholar 

  • Rubio-Diaz, D. E., Francis, D. M., & Rodriguez-Saona, L. E. (2011). External calibration models for the measurement of tomato carotenoids by infrared spectroscopy. Journal of Food Composition and Analysis, 24(1), 121–126.

    Article  CAS  Google Scholar 

  • Shu, B., Yu, W., Zhao, Y., & Liu, X. (2006). Study on microencapsulation of lycopene by spray-drying. Journal of Food Engineering, 76, 664–669.

    Article  CAS  Google Scholar 

  • Silva, D. F., Favaro-Trindade, C. S., Rocha, G. A., & Thomazini, M. (2012). Microencapsulation of lycopene by gelatin-pectin complex coacervation. Journal of Food Processing and Preservation, 36(2), 185–190.

    Article  CAS  Google Scholar 

  • Tan, B., & Soderstrom, D. N. (1989). Qualitative aspects of UV–Vis spectrophotometry of β-carotene and lycopene. Journal of Chemical Education, 66(3), 258–260.

    Article  CAS  Google Scholar 

  • Torres-Giner, S., Ocio, M. J., & Lagaron, J. M. (2008). Development of active antimicrobial fibre-based chitosan polysaccharide nanostructures using electrospinning. Engineering in Life Sciences, 8(3), 303–314.

    Article  CAS  Google Scholar 

  • Vlachos, N., Skopelitis, Y., Psaroudaki, M., Konstantinidou, V., Chatzilazarou, A., & Tegou, E. (2006). Applications of Fourier transform-infrared spectroscopy to edible oils. Analytica Chimica Acta, 573–574, 459–465.

    Article  Google Scholar 

  • Wolkers, W. F., Oliver, A. E., Tablin, F., & Crowe, J. H. (2004). A Fourier-transform infrared spectroscopy study of sugar glasses. Carbohydrate Research, 339, 1077–1085.

    Article  CAS  Google Scholar 

  • Xue, F., Li, C., Liu, Y., Zhu, X., Pan, S., & Wang, L. (2013). Encapsulation of tomato oleoresin with zein prepared from corn gluten meal. Journal of Food Engineering, 119, 439–445.

    Article  CAS  Google Scholar 

  • Zhang, Y., & Zhong, Q. (2013). Probing the binding between norbixin and dairy proteins by spectroscopy methods. Food Chemistry, 139(1–4), 611–616.

    Article  CAS  Google Scholar 

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The authors thank the Spanish MINECO projects AGL2012-30647 and FUN-C-FOOD (CSD2007-00063) for financial support. The Electronic Microscopy department at the SCIE from the University of Valencia is also acknowledged for the support with SEM analyses.

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Correspondence to Amparo Lopez-Rubio.

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Pérez-Masiá, R., Lagaron, J.M. & Lopez-Rubio, A. Morphology and Stability of Edible Lycopene-Containing Micro- and Nanocapsules Produced Through Electrospraying and Spray Drying. Food Bioprocess Technol 8, 459–470 (2015).

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