Osmotic Dehydration of Pineapple with Impregnation of Sucrose, Calcium, and Ascorbic Acid


Mass transfer was evaluated during osmotic dehydration of pineapple in solutions with until four components aiming to investigate the solutes concentration influence on impregnation. In the first step, the experimental trials for optimization of solution concentration were based on 23 factorial design. In the second step, effective diffusion coefficients were determined. Equations representing the influence of the concentration of sucrose, calcium lactate, and ascorbic acid in osmotic solutions on water loss and gains of sucrose, calcium, and vitamin C were found. Results showed that both calcium lactate and sucrose concentration affected calcium and sucrose gain. On the other hand, only vitamin C gain was significantly affected by the ascorbic acid concentration in the studied concentration range. However, when comparing diffusivities in pineapple immersed in sucrose solutions, with and without calcium lactate, with and without ascorbic acid, it was possible to verify that diffusivities of water, sugar, and calcium increased in presence of ascorbic acid in solution. Calcium in solution diminished the water and sucrose diffusivities. High calcium and vitamin C contents were obtained in 1 h immersion in the solutions studied.

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Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5


M :

mass (kg)

M 0 :

initial mass (kg)


total mass variation in relation to initial mass (dimensionless)

R2 :

determination coefficient (dimensionless)


sugar gain in relation to initial mass (dimensionless)

ΔG Ca :

calcium gain in relation to initial mass (dimensionless)

ΔG VitC :

Ascorbic acid gain in relation to initial mass (dimensionless)

ΔW :

water loss in relation to initial mass (dimensionless)

β 0 β 1 β 2, β 3, β 12, β 13, β 23, β 123 :

estimated regression coefficient of the Eq. (1)

w w :

water content

w SUC :

sucrose content

w Ca :

calcium content

w VitC :

ascorbic acid content

\( \overline{{{w_i}}}(t) \) :

mean concentration of the component for a time (t)


dimensionless sucrose content

ADM (Ca ):

dimensionless calcium content

ADM (VitC) :

dimensionless vitamin C content

ADM (w) :

dimensionless water content


sucrose concentration (%)


calcium lactate concentration (%)

VitC :

Ascorbic acid concentration (%)

Coded variables:

x 1i ; x 2i ; x 3i ; (1 = SUC, 2 = LAC, 3 = VitC)

Response variables:

Y i (Y 1 = water loss (ΔW); Y 2 sucrose gain (ΔG SUC ); Y 3 calcium gain (ΔG Ca ); Y 4 Ascorbic acid gain (ΔG VitC ));

Calc :





osmotically dehydrated

S :


Ca :


VitC :

ascorbic acid (vitamin C)

w :



initial state


  1. Aminzadeh, R., Sargolzaei, J., & Abarzani, M. (2010). Preserving melons by osmotic dehydration in a ternary system followed by air-drying. Food and Bioprocess Technology, 5(4), 1305–1316.

    Article  Google Scholar 

  2. Anino, S. V., Salvatori, D. M., & Alzamora, S. M. (2006). Changes in calcium level and mechanical properties of apple tissue due to impregnation with calcium salts. Food Research Internacional, 39, 154–164.

    CAS  Article  Google Scholar 

  3. AOAC - Association of Official Analytical Chemists. (1970). Official Methods of Analysis of the Association of Official Analytical Chemists (11th ed.). Arlington: Association of Official Analytical Chemists AOAC.

    Google Scholar 

  4. AOAC - Association of Official Analytical Chemists. (1984). Official Methods of Analysis of the Association of Official Analytical Chemists (14th ed.). Arlington: Association of Official Analytical Chemists AOAC.

    Google Scholar 

  5. AOAC - Association of Official Analytical Chemists (1995) Official Methods of Analysis of the Association of Official Analytical Chemists, 16th ed., v. 1, Arlington: Association of Official Analytical Chemists A.O.A.C., chapter 3. p. 4. (method 985.01).

  6. Atkins, C. D., & Rouse, A. H. (1953). Time-temperature relationships for heat inactivation of pectinesterase in citrus juices. Food Technology, Chicago, 7(12), 489–491.

    CAS  Google Scholar 

  7. Barrera, C., Betoret, N., Corell, P., & Fito, P. (2009). Effect of osmotic dehydration on the stabilization of calcium-fortified apple slices (var. Granny Smith): Influence of operating variables on process kinetics and compositional changes. Journal of Food Engineering, 92, 416–424.

    CAS  Article  Google Scholar 

  8. Benassi, M. T., & Antunes, A. J. (1988). A comparison of meta-phosphoric and oxalic acids as extractant solutions for the determination of vitamin C in selected vegetables. Arquivos de Biologia e Tecnologia, 31(4), 507–513.

    CAS  Google Scholar 

  9. Collet, L. S. F. C. A., Shigeoka, D. S., Badolato, G. G., & Tadini, C. C. (2005). A kinetic study on pectinesterase inactivation during continuous pasteurization of orange juice. Journal of Food Engineering, 69, 125–129.

    Article  Google Scholar 

  10. Crank, J. (1975). The Mathematics of Diffusion (2nd ed.). London: Clarendon Press-Oxford.

    Google Scholar 

  11. Crapiste, G. H., Whitaker, S., & Rotstein, E. (1988). Drying a cellular material - I. A mass transfer theory. Chemical Engineering Science, 43(11), 2919–2928.

    CAS  Article  Google Scholar 

  12. Ferrari, C. C., Carmello-Guerreiro, S. M., Bolini, H. M. A., & Hubinger, M. D. (2010). Structural changes, mechanical properties and sensory preference of osmodehydrated melon pieces with sucrose and calcium lactate solutions. International Journal of Food Properties, 13, 112–130.

    CAS  Article  Google Scholar 

  13. Fito, P., Chiralt, A., Betoret, N., Gras, M., Cháfer, M., Martínez-Monzó, J., Andrés, A., & Vidal, D. (2001). Vacuum impregnation and osmotic dehydration in matrix engineering. Application in functional fresh food development. Journal of Food Engineering, 49, 175–183.

    Article  Google Scholar 

  14. Garcia, C. C., Mauro, M. A., & Kimura, M. (2007). Kinetics of osmotic dehydration and air drying of pumpkins (Cucurbita moschata). Journal of Food Engineering, 82, 284–291.

    Article  Google Scholar 

  15. Henrion, P. N. (1964). Diffusion in the sucrose + water system. Transactions of the Faraday Society, 60, 72–82.

    CAS  Article  Google Scholar 

  16. Katz, F. (2000). Research priorities more toward healthy and safe. Food Technology, 54(12), 42–44.

    Google Scholar 

  17. Lenart, A. (1996). Osmo-convective drying of fruits and vegetables: technology and application. Drying Technology, 14(2), 391–413.

    CAS  Article  Google Scholar 

  18. Mastrantonio, S. D. S., Pereira, L. M., & Hubinger, M. D. (2005). Osmotic dehydration kinetics of guavas in maltose solutions with calcium salt. Alimentos e Nutrição, 16(4), 309–314.

    CAS  Google Scholar 

  19. Mavroudis, N. E., Gidley, M. J., & Sjöholm, I. (2012). Osmotic processing: effects of osmotic medium composition on the kinetics and texture of apple tissue. Food Research International, 48, 839–847.

    CAS  Article  Google Scholar 

  20. Meyer, R. H. (1971). Response Surface Methodology (pp. 126–175). Boston: Allen and Bacon.

    Google Scholar 

  21. Monerat, S. M., Pizzi, T. R. M., Mauro, M. A., & Menegalli, F. C. (2010). Osmotic dehydration of apples in sugar/salt solutions: concentration profile and effective diffusion coefficients. Journal of Food Engineering, 100, 604–612.

    Article  Google Scholar 

  22. Montgomery, D. C. (1991). Análisis y Diseño Experimental. Mexico: Grupo Editorial Iberoamerica.

    Google Scholar 

  23. Pereira, L. M., Ferrari, C. C., Mastrantonio, S. D. S., & Rodrigues, A. C. C. (2006). Kinetic aspects, texture, and color evaluation of some tropical fruits during osmotic dehydration. Drying Technology, 24, 475–484.

    CAS  Article  Google Scholar 

  24. Qi, H., Le Maguer, M., & Sharma, S. K. (1998). Design and selection of processing conditions of a pilot scale contactor for continuous osmotic dehydration of carrots. Journal of Food Processing and Engineering, 21, 75–88.

    Article  Google Scholar 

  25. Ramallo, L. A., & Mascheroni, R. H. (2010). Dehydrofreezing of pineapple. Journal of Food Engineering, 99, 269–275.

    Article  Google Scholar 

  26. Raoult-Wack, A. L. (1994). Recent advances in the osmotic dehydration of foods. Trends in Food Science and Technology, 5, 255–260.

    Article  Google Scholar 

  27. Robbers, M., Singh, R. P., & Cunha, L. M. (1997). Osmotic-convective dehydrofreezing process for drying kiwifruit. Journal of Food Science. Chicago, 62(5), 1039–1047.

    CAS  Article  Google Scholar 

  28. Saputra, D. (2001). Osmotic dehydration of pineapple. Drying Technology, 19, 415–425.

    CAS  Article  Google Scholar 

  29. Sereno, A. M., Moreira, R., & Martinez, E. (2001). Mass transfer coefficients during osmotic dehydration of apple in single and combined aqueous solutions of sugar and salt. Journal of Food Engineering, 47, 43–49.

    Article  Google Scholar 

  30. Silva, W.P. and Silva, C.M.D.P.S. (2008) Prescribed adsorption–desorption V 2.2, online, available from world wide web: http://zeus.df.ufcg.edu.br/labfit/Prescribed.htm, date of access: Septembre, 20

  31. Silva, A. C., Silva, C. R., Costa, L. M. S., Barros, N. A. M., Viana, A. S., Koblitz, M. G. B., & Souza, F. V. D. (2011a). Use of response surface methodology for optimization of the extraction of enzymes from pineapple pulp. Acta Horticulturae, 902, 575–584.

    Google Scholar 

  32. Silva, K. S., Caetano, L. C., Garcia, C. C., Romero, J. T., Santos, A. B., & Mauro, M. A. (2011b). Osmotic dehydration process for low temperature blanched pumpkin. Journal of Food Engineering, 105, 56–64.

    CAS  Article  Google Scholar 

  33. Singh, B., Kumar, A., & Gupta, A. K. (2007). Study of mass transfer kinetics and effective diffusivity during osmotic dehydration of carrot cubes. Journal of Food Engineering, 79, 471–480.

    CAS  Article  Google Scholar 

  34. Wilinska, A.; Rodrigues, A. S. F.; Bryjak, J.; Polakovic, M. (2008) Thermal inactivation of exogenous pectin methylesterase in apple and cloudberry juices. Journal of Food Engineering 85, 459exog

  35. Zemke-White, W. L., Clements, K. D., & Harris, P. J. (2000). Acid lysis of macroalgae by marine herbivorous fishes: Effects of acid pH on cell wall porosity. Journal of Experimental Marine Biology and Ecology, 245, 57–68.

    CAS  Article  Google Scholar 

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The authors would like to thank CAPES for the scholarship, PURAC Synthesis (Brazil) and Prozyn (Brazil).

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Correspondence to Keila S. Silva.

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Silva, K.S., Fernandes, M.A. & Mauro, M.A. Osmotic Dehydration of Pineapple with Impregnation of Sucrose, Calcium, and Ascorbic Acid. Food Bioprocess Technol 7, 385–397 (2014). https://doi.org/10.1007/s11947-013-1049-0

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  • Osmotic dehydration
  • Calcium
  • Ascorbic acid
  • Pineapple
  • Sucrose gain
  • Impregnation