Abstract
Yellow tea is the least investigated type of tea (Camellia sinensis), therefore, this study is focused on characterization of bioactive content of yellow tea extracts as well as application of inovative extraction techniques (ultrasound bath and probe) compared to conventional extraction, in water and aqueous ethanol media. Comparison of obtained extracts was based on total flavonoid (TFC) and nonflavonoid (TNC) content, HPLC analysis of individual polyphenols and methylxanthines, and antioxidant capacity. The highest TFC was detected in extracts obtained by ultrasound probe assisted extraction in aqueous ethanol as an extraction medium and the lowest in extracts obtained by ultrasound bath extraction (water and aqueous ethanol) with a trend of increase with prolonged extraction time. Conventional extraction was successful when combined with 75% aqueous ethanol as an extraction medium. Results of HPLC analysis and antioxidant capacity assays were generally in compliance with these results. This study proves that ultrasound probe extraction could succesfully be used for extraction of polyphenols and methylxanthines from yellow tea.
This is a preview of subscription content, access via your institution.



References
Beltz, L. A., Bayer, D. K., Moss, A. L., & Simet, I. M. (2006). Mechanisms of cancer prevention by green and black tea polyphenols. Anti-Cancer Agents in Medicinal Chemistry, 6(5), 389–406.
Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239, 70–76.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. LWT—Food Science and Technology, 28, 25–30.
Chang, C. J., Chiu, K.-L., Chen, Y.-L., & Chang, C.-Y. (2000). Separation of catechins from green tea using carbon dioxide extraction. Food Chemistry, 68, 109–113.
Chen, L., Chen, Q., Zhang, Z., & Wan, X. (2009). A novel colorimetric determination of free amino acids content in tea infusions with 2,4-dinitrofluorobenzene. Journal of Food Composition and Analysis, 22, 137–141.
Dawidowicz, A. L., & Wianowska, D. (2005). PLE in the analysis of plant compounds: Part I. The application of PLE for HPLC analysis of caffeine in green tea leaves. Journal of Pharmaceutical and Biomedical Analysis, 37, 1155–1159.
Friedman, M., Kim, S.-Y., Lee, S.-J., Han, G.-P., Han, J.-S., Lee, K.-R., et al. (2005). Distribution of catechins, theaflavins, caffeine, and theobromine in 77 teas consumed in the United States. Journal of Food Science, 70(9), C550–C559.
Friedman, M., Levin, C. E., Choi, S.-H., Kozukue, E., & Kozukue, N. (2006). HPLC analysis of catechins, theaflavins, and alkaloids in commercial teas and green tea dietary supplements: comparison of water and 80% ethanol/water extracts. Journal of Food Science, 71(6), C328–C337.
Fukino, Y., Shimbo, M., Aoki, N., Okubo, T., & Iso, H. (2005). Randomized controlled trial for an effect of green tea consumption on insulin resistance and inflammation markers. Journal of Nutritional Science and Vitaminology, 51, 335–342.
Goto, T., Yoshida, Y., Amano, I., & Horie, H. (1996). Chemical composition of commercially available Japanese green tea. Foods and Food Ingredients Journal of Japan, 170, 46–51.
Horžić, D., Komes, D., Belščak, A., Kovačević Ganić, K., Iveković, D., & Karlović, D. (2009). The composition of polyphenols and methylxanthines in teas and herbal infusions. Food chemistry, 115, 441–448.
Jun, X. (2009). Caffeine extraction from green tea leaves assisted by high pressure processing. Journal of Food Engineering, 94, 105–109.
Jun, X., Shuo, Z., Bingbing, L., Rui, Z., Ye, L., Deji, S., et al. (2010). Separation of major catechins from green tea by ultrahigh pressure extraction. International Journal of Pharmaceutics, 386(1–2), 229–231.
Khan, M. K., Abert-Vian, M., Fabiano-Tixier, A.-S., Dangles, O., & Chemat, F. (2010). Ultrasound-assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry, 119(2), 851–858.
Komes, D., Horžić, D., Belščak, A., Kovačević Ganić, K., & Vulić, I. (2010). Green tea preparation and its influence on the content of bioactive compounds. Food research international, 43(1), 167–176.
Kramling, T. E., & Singleton, V. E. (1969). An estimate of the nonflavonoid polyphenols in wines. American Journal of Enology and Viticulture, 20(2), 86–92.
Lachman J, Hosnedl V, Pivec V & Orsak M (1998). Polyphenols in cereals and their positive and negative role in human and animal nutrition. In: Proceedings of conference cereals for human health and preventive nutrition, pp. 118–125. Brno, Czech Republic.
Lin, S.-D., Liu, E.-H., & Mau, J.-L. (2008). Effect of different brewing methods on antioxidant properties of steaming green tea. LWT—Food Science and Technology, 41, 1616–1623.
Mandel, S. A., Amit, T., Kalfon, L., Reznichenko, L., & Youdim, M. B. H. (2008). Targeting multiple neurodegenerative diseases etiologies with multimodalacting green tea catechins. Journal of Nutrition, 138(8), 1578S–1583S.
Margulis, M. A., & Margulis, I. M. (2003). Calorimetric method for measurement of acoustic power absorbed in a volume of a liquid. Ultrasonics Sonochemistry, 10, 343–345.
Mason, T. J. (1998). Power ultrasound in food processing—the way forward. In M. J. W. Povey & T. J. Mason (Eds.), ultrasound in food processing (pp. 105–126). London, UK: Thomson Science.
Mason, T. J., & Zhao, Y. Y. (1994). Enhanced extraction of tea solids using ultrasound. Ultrasonics, 32(5), 375–377.
Mejria, M., Souissib, A. B., Aroulmojib, V., & Rogéb, B. (2009). Hydration and self-association of caffeine molecules in aqueous solution: comparative effects of sucrose and β-cyclodextrin. Spectrochimica Acta A, 73, 6–10.
Najafia, N. M., Hamidb, A. S., & Afshina, R. K. (2003). Determination of caffeine in black tea leaves by Fourier transform infrared spectrometry using multiple linear regression. Microchemical Journal, 75, 151–158.
Nkhili, E., Tomao, V., El Hajji, H., El Boustani, E.-S., Chemat, F., & Dangles, O. (2009). Microwave-assisted water extraction of green tea polyphenols. Phytochemical Analysis, 20, 408–415.
Paniwnyk, L., Beaufoy, E., Lorimer, J. P., & Mason, T. J. (2001). The extraction of rutin from flower buds of Sophora japonica. Ultrasonics Sonochemistry, 8(3), 299–301.
Paniwnyk, L., Cai, H., Albu, S., Mason, T. J., & Cole, R. (2009). The enhancement and scale up of the extraction of anti-oxidants from Rosmarinus officinalis using ultrasound. Ultrasonics Sonochemistry, 16(2), 287–292.
Potterat, O. (1997). Antioxidants and free radical scavengers of natural origin. Current Organic Chemistry, 1, 415–440.
Rall, T. W. (1993). The pharmacological basis of therapeutics (pp. 618–637). New York, NY, USA: McGraw-Hill.
Rusak, G., Komes, D., Likić, S., Horžić, D., & Kovač, M. (2008). Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used. Food Chemistry, 110(4), 852–858.
Salisova, M., Toma, S., & Mason, T. J. (1997). Comparison of conventional and ultrasonically-assisted extraction of pharmaceutically active compounds from Salvia officinalis. Ultrasonics Sonochemistry, 4, 131–134.
Seeley, D., Mills, E. J., Wu, P., Verma, S., & Guyatt, G. H. (2005). The effects of green tea consumption on incidence of breast cancer and recurrence of breast cancer: a systematic review and meta-analysis. Integrative Cancer Therapies, 4, 144–155.
Sharangi, A. B. (2009). Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)—a review. Food Research International, 42, 529–535.
Suslick, K. S. (1988). Ultrasound: its chemical, physical, and biological effects. In K. S. Suslick (Ed.), Ultrasound, VCH Press, New York.
Vinatoru, M., Toma, M., & Mason, T. J. (1999). Ultrasound-assisted extraction of bioactive principles from plants and their constituents. Advances in Sonochemistry, 5, 209–247.
Wanasundara, P. K. J. P. D., Shahidi, F., & Shukla, V. K. S. (1997). Endogenous antioxidants from oilseeds and edible oils. Food Reviews International, 13, 225–292.
Wang, L.-F., Lee, J.-Y., Chung, J.-O., Baik, J.-H., So, S., & Park, S.-K. (2008). Discrimination of teas with different degrees of fermentation by SPME-GC analysis of the characteristic volatile flavour compounds. Food Chemistry, 109, 196–206.
Wanga, D., Lua, J., Miaob, A., Xiea, Z., & Yanga, D. (2008). HPLC-DAD-ESI-MS/MS analysis of polyphenols and purine alkaloids in leaves of 22 tea cultivars in China. Journal of Food Composition and Analysis, 21, 361–369.
Xia, T., Shi, S., & Wan, X. (2006). Impact of ultrasonic-assisted extraction on the chemical and sensory quality of tea infusion. Journal of Food Engineering, 74, 557–560.
Acknowledgement
This work was supported by the Ministry of Science, Education and Sports, Republic of Croatia project 058 3470.
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was supported by the Ministry of Science, Education and Sports, Republic of Croatia project 058 3470.
Rights and permissions
About this article
Cite this article
Horžić, D., Jambrak, A.R., Belščak-Cvitanović, A. et al. Comparison of Conventional and Ultrasound Assisted Extraction Techniques of Yellow Tea and Bioactive Composition of Obtained Extracts. Food Bioprocess Technol 5, 2858–2870 (2012). https://doi.org/10.1007/s11947-012-0791-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11947-012-0791-z
Keywords
- Yellow tea
- HPLC
- Antioxidants
- Extraction
- Ultrasound