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Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System

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Abstract

The purification of lipoxygenase in soybeans (richest known source) was attempted by precipitation, using polyethylene glycol, followed by aqueous two-phase extraction (ATPE). The preliminary purification by precipitation was standardized which resulted in two-fold purification. The influence of process parameters such as phase forming salt, molecular weight of phase forming polymer, tie line length, and phase volume ratio on the partitioning behavior of lipoxygenase during ATPE was investigated. The aqueous two-phase system of polyethylene glycol 6000 and ammonium sulfate, with inherent pH of 5.2, was found to be most suitable. The tie line length of 41.25% and volume ratio of 1.7 have resulted in 125% activity recovery with the overall purification factor of 4.38 compared to crude enzyme extract.

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Acknowledgements

Authors thank Dr. V. Prakash, Director, CFTRI, Mysore, for his encouragement and keen interest in the area of downstream processing. MCL and MCM gratefully acknowledge CSIR, Government of India, for the Senior Research Fellowship.

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Authors and Affiliations

  1. Department of Food Engineering, Central Food Technological Research Institute, Council of Scientific and Industrial Research (CSIR), Mysore, 570 020, India

    M. C. Lakshmi, M. C. Madhusudhan & K. S. M. S. Raghavarao

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  1. M. C. Lakshmi
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  2. M. C. Madhusudhan
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  3. K. S. M. S. Raghavarao
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Correspondence to K. S. M. S. Raghavarao.

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Lakshmi, M.C., Madhusudhan, M.C. & Raghavarao, K.S.M.S. Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System. Food Bioprocess Technol 5, 193–199 (2012). https://doi.org/10.1007/s11947-009-0278-8

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