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Study of the Extraction, Concentration, and Partial Characterization of Lipases Obtained from Penicillium verrucosum using Solid-State Fermentation of Soybean Bran

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Abstract

Microbial lipases constitute an important group of enzymes with high biotechnological potential, but their application is limited due to production costs. The present study aimed to evaluate the extraction conditions, concentration with ammonium sulfate, and the partial characterization of the enzyme extracts (in terms of the optimum temperature and pH and their stability at low temperatures). It was shown that the optimal extraction conditions were established at 37 °C and pH of 7.0 and that the best conditions for precipitation with ammonium sulfate were with 60% saturation for 5 h. In the partial characterization of the concentrated extract, the optima conditions were obtained at 42 °C and pH of 8.5. In a study carried out with the concentrated enzymatic extract, the hydrolytic activity was shown to be practically the same as the initial value after 91 days of storage at both 4 and −10 °C.

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Acknowledgments

The authors would like to thank CNPq, CAPES/PROCAD, and Intecnial SA for the financial support and scholarships.

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Authors and Affiliations

  1. Programa de Mestrado em Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões–Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, Rio de Janeiro, Brazil

    Silvana Menoncin, Natália Molossi Domingues, Geciane Toniazzo, Rogério Luis Cansian, J. Vladimir Oliveira, Marco Di Luccio, Débora de Oliveira & Helen Treichel

  2. Depto. de Bioquímica–IQ/UFRJ, Centro de Tecnologia, Bl. A, Sala 549-2, Rio de Janeiro, 21945-900, Rio de Janeiro, Brazil

    Denise Maria Guimarães Freire

Authors
  1. Silvana Menoncin
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  2. Natália Molossi Domingues
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  3. Denise Maria Guimarães Freire
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  4. Geciane Toniazzo
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  5. Rogério Luis Cansian
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  6. J. Vladimir Oliveira
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  7. Marco Di Luccio
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  8. Débora de Oliveira
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  9. Helen Treichel
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Correspondence to Helen Treichel.

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Menoncin, S., Domingues, N.M., Freire, D.M.G. et al. Study of the Extraction, Concentration, and Partial Characterization of Lipases Obtained from Penicillium verrucosum using Solid-State Fermentation of Soybean Bran. Food Bioprocess Technol 3, 537–544 (2010). https://doi.org/10.1007/s11947-008-0104-8

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  • DOI: https://doi.org/10.1007/s11947-008-0104-8

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