Skip to main content
Log in

Front-Face Fluorescence Spectroscopy as a Rapid and Non-Destructive Tool for Differentiating Between Sicilo–Sarde and Comisana Ewe’s Milk During Lactation Period: A Preliminary Study

  • Published:
Food and Bioprocess Technology Aims and scope Submit manuscript

Abstract

The objective of this study was to assess the potential of front-face fluorescence spectroscopy for differentiating between two genotypes (Comisana and Sicilo–Sarde) of ewe’s milk collected during lactation period. Physico-chemical analyses and fluorescence spectra were performed on milk samples during the first 15 weeks of lactation period. Regarding fluorescence spectra, aromatic amino acids and nucleic acids (AAA+NA), tryptophan, and vitamin A were recorded on milk samples after excitation set at 250, 290, and 322 nm, respectively. Emission spectra of vitamin A were scanned after emission set at 410 nm. Among the investigated intrinsic probes, only the principal component analysis (PCA) performed on AAA+NA allowed a good discrimination between milks produced from Comisana and those collected from Sicilo–Sarde ewes. Also, the PCA carried out on the physico-chemical parameters did not allow any discrimination between milk samples according to their genotypes. It can be concluded that emission spectra of AAA+NA could be considered as fingerprints allowing a good identification of milk samples according to ewe’s genotypes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aleandri, R., Buttazoni, L. G., & Schneider, J. C. (1990). The effects of milk protein polymorphism on milk components and cheese producing ability. Journal of Dairy Science, 73, 241–255.

    Article  Google Scholar 

  • Antunovié, Z., Steiner, Z., Senčié, Đ., Mandié, M., & Klapec, T. (2001). Changes of ewe milk composition depending on lactation stage and feeding season. Czech Journal of Animal Science, 46, 75–82.

    Google Scholar 

  • Bencini, R., Hartmann, P. E., & Lightfoot, R. J. (1992). Comparative dairy potential of Awassi x Merino and Merino ewes. Proceedings of the Australian Association of Animal Breeding and Genetics, 10, 114–117.

    Google Scholar 

  • Bencini, R., & Pulina, G. (1997). The quality of sheep milk: A review. International Journal of Sheep and Wool Science, 45, 182–220.

    Google Scholar 

  • Bertrand, D., & Scotter, C. N. G. (1992). Application of multivariate analyses to NIR spectra of gelatinized starch. Applied Spectroscopy, 46, 1420–1425.

    Article  CAS  Google Scholar 

  • Casu, S., & Boyazoglu, J. (1990). La production ovine laitière méditerranéenne: Régions de production, types génétiques utilisés, systèmes d’élevage et perspectives d’avenir. Options Méditerranéennes - Série A, 12, 19–24.

    Google Scholar 

  • Chiofalo, V., Micari, P., Chiofalo, V., & Giunta, P. (1989). Chemical and coagulation parameters in the milk of the Sarda and Comisana breeds. Proceeding of the Italian Society of Veterinary Science, 43, 1851–1860.

    Google Scholar 

  • Dufour, E., & Riaublanc, A. (1997). Potentiality of spectroscopic methods for the characterisation of dairy products, I- Front-face fluorescence study of raw, heated and homogenised milks. Le Lait, 77, 657–670.

    Article  CAS  Google Scholar 

  • Geenty, K. G. (1979). Lactation performance, growth and carcass composition of sheep. 1. Milk production, milk composition and live weights of Romney, Corriedale, Dorset, Romney x Dorset and Dorset x Romney ewes in relation to the growth of their lambs. New Zealand Journal of Agricultural Research, 22, 241–250.

    Google Scholar 

  • Genot, C., Tonetti, F., Montenay-Garestier, T., & Drapron, R. (1992). Front face fluorescence applied to structural studies of proteins and lipid-protein interactions of viscoelastic food products. 1. Designing of front-face adaptor and validity of front face fluorescence measurements. Sciences des Aliments, 12, 199–212.

    CAS  Google Scholar 

  • Herbert, S. (1999). Caractérisation de la structure moléculaire et microscopique de fromages à pâte molle. Analyse multivariée des données structurales en relation avec la texture. PhD thesis, Ecole Doctorale Chimie Biologie de l’Université de Nantes, Nantes, France.

  • Karoui, R., Cartaud, G., & Dufour, E. (2006). Front-face fluorescence spectroscopy as a rapid and nondestructive tool for differentiating various cereal products: A preliminary investigation. Journal of Agricultural and Food Chemistry, 54, 2027–2034.

    Article  CAS  Google Scholar 

  • Karoui, R., Martin, B., & Dufour, E. (2005). Potentiality of front face fluorescence spectroscopy to determine the geographic origin of milks from Haute-Loire department (France). Le Lait, 85, 223–236.

    Article  Google Scholar 

  • Karoui, R., Mazerolles, G., Dufour, E, & , . (2003). Spectroscopic techniques coupled with chemometric tools for structure and texture determinations in dairy products: A review. International Dairy Journal, 13, 607–620.

    Article  Google Scholar 

  • Kulmyrzaev, A., Levieux, D., & Dufour, E. (2005). Front-face fluorescence spectroscopy allows the characterization of mild heat treatment applied to milk. Relations with the denaturation of milk proteins. Journal of Agricultural and Food Chemistry, 53, 502–507.

    Article  CAS  Google Scholar 

  • Jolliffe, I. T. (1986). Principal component analysis. New York, USA: Springer.

    Google Scholar 

  • Moore, R. W. (1996a). Genetic factors affecting the milk intake of lambs. Australian Journal of Agricultural Research, 17, 191–199.

    Article  Google Scholar 

  • Moore, R. W. (1996b). Milk quality in Merino and Corriedale ewes. Australian Journal of Agricultural Research, 17, 201–208.

    Article  Google Scholar 

  • Pirisi, A., Piredda, G., Papoff, C. M., Di Salvo, R., Pintus, S., Garro, G., Ferranti, P., & Chianese, L. (1999). Effect of sheep alpha S1-casein CC, CD and DD genotypes on milk composition and cheese making properties. Journal of Dairy Research, 66, 409–419.

    Article  CAS  Google Scholar 

  • Rouissi, H., Dridi , S., Kammoun, M., De Baerdemaeker, J., & Karoui, R. (2007). Front face fluorescence spectroscopy: a rapid tool for determining the effect of replacing soybean meal with scotch bean in the ration on the quality of Sicilo-Sarde ewe’s milk during lactation period. European Food Research and Technology (in press).

  • Sahan, N., Say, D., & Kaçar, A. (2005). Changes in chemical and mineral contents of Awassi ewe’s milk during lactation. Turkish Journal of Veterinary and Animal Sciences, 29, 589–593.

    CAS  Google Scholar 

  • Schamberger, G. P., & Labuza, T. P. (2006). Evaluation of front-face fluorescence for assessing thermal processing of milk. Journal of Food Science, 71, 69–74.

    Article  Google Scholar 

  • Strasburg, G. M., & Ludescher, R. D. (1995). Theory and application of fluorescence spectroscopy in food research. Trends in the Food Science and Technology, 6, 69–75.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Romdhane Karoui.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zaïdi, F., Rouissi, H., Dridi, S. et al. Front-Face Fluorescence Spectroscopy as a Rapid and Non-Destructive Tool for Differentiating Between Sicilo–Sarde and Comisana Ewe’s Milk During Lactation Period: A Preliminary Study. Food Bioprocess Technol 1, 143–151 (2008). https://doi.org/10.1007/s11947-007-0028-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11947-007-0028-8

Keywords

Navigation