Pluripotent Stem Cells and Skeletal Regeneration—Promise and Potential
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The bone is a regenerative tissue, capable of healing itself after fractures. However, some circumstances such as critical-size defects, malformations, and tumor destruction may exceed the skeleton’s capacity for self-repair. In addition, bone mass and strength decline with age, leading to an increase in fragility fractures. Therefore, the ability to generate large numbers of patient-specific osteoblasts would have enormous clinical implications for the treatment of skeletal defects and diseases. This review will highlight recent advances in the derivation of pluripotent stem cells, and in their directed differentiation towards bone-forming osteoblasts.
KeywordsBone Skeleton regeneration Stem cells
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Conflict of Interest
Dr. Joy Y. Wu has received research support from the National Institute of Health and was funded by NIH grant OD008466.
Human and Animal Rights and Informed Consent
All studies by Dr. Joy Y. Wu involving animal and/or human subjects were performed after approval by the appropriate institutional review boards. When required, written informed consent was obtained from all participants.
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- 20.Bourne S, Polak JM, Hughes SP, Buttery LD. Osteogenic differentiation of mouse embryonic stem cells: differential gene expression analysis by cDNA microarray and purification of osteoblasts by cadherin-11 magnetically activated cell sorting. Tissue Eng. 2004;10(5–6):796–806.PubMedCrossRefGoogle Scholar
- 27.•Marolt D, Campos IM, Bhumiratana S, Koren A, Petridis P, Zhang G, et al. Engineering bone tissue from human embryonic stem cells. Proc Natl Acad Sci U S A. 2012;109(22):8705–9. This article reported the use of tissue engineering techniques to promote bone formation from ES cells. PubMedCentralPubMedCrossRefGoogle Scholar
- 64.•Fox IJ, Daley GQ, Goldman SA, Huard J, Kamp TJ, Trucco M. Stem cell therapy. Use of differentiated pluripotent stem cells as replacement therapy for treating disease. Science. 2014;345(6199):1247391. This article reviews the potential clinical applications of iPSC technology to regenerative medicine. PubMedCentralPubMedCrossRefGoogle Scholar
- 70.•Phillips MD, Kuznetsov SA, Cherman N, Park K, Chen KG, McClendon BN, et al. Directed differentiation of human induced pluripotent stem cells toward bone and cartilage: in vitro versus in vivo assays. Stem Cells Transl Med. 2014;3(7):867–78. This article compared the osteogenic differentiation capacity of iPSCs from fibroblasts vs bone marrow stromal cells, assayed by both in vitro and in vivo methods. PubMedCentralPubMedCrossRefGoogle Scholar
- 74.••Kanke K, Masaki H, Saito T, Komiyama Y, Hojo H, Nakauchi H, et al. Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions. Stem Cell Rep. 2014;2(6):751–60. This article reported the use of small molecule compounds to recapitulate the developmental stages of osteoblast differentiation starting with pluripotent stem cells. CrossRefGoogle Scholar