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Synergic Inhibition of Lung Carcinoma 95-D Cell Proliferation and Invasion by Combination with (−)-Epigallocatechin-3-Gallate and Ascorbic Acid

  • Yan Ma
  • Jingya Yang
  • Shiyi Li
  • Wenjuan Yu
Chemistry and Biology
  • 16 Downloads

Abstract

In this study, the anti-invasion effects of (−)-epigallocatechin- 3-gallate (EGCG) mixed with ascorbic acid (Vc) on human lung carcinoma 95-D cells in vitro were examined and the synergism of the combination of EGCG and Vc was evaluated. Soft agar colony formation assay, cell migration assay, invasion assay, western blot analysis of NF-κB, in situ detection of cellular oxidative stress, and statistical analysis were assessed. The results showed that combining EGCG with Vc could inhibit clone forming rate of 95-D cell by 73.2%, reduce the migration ability of 95-D cell by 65.9%, and decrease the intracellular reactive oxygen species(ROS) level by 76.8%. The results of western blot proved that Vc enhanced the activity of EGCG in inhibiting NF-κB localization. It is speculated that the combination of EGCG and Vc can strongly suppress the proliferation and metastasis of lung carcinoma cells in a synergic manner, possibly with a mechanism associated with the scavenging of reactive oxygen species.

Key words

(−)-epigallocatechin-3-gallate (EGCG) reactive oxygen species (ROS) ascorbic acid (Vc) cancer metastasis 

CLC number

R 73 

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References

  1. [1]
    Grove K A, Lambert J D. Laboratory, epidemiological, and human intervention studies show that tea (Camellia sinensis) may be useful in the prevention of obesity[J]. Journal of Nutrition, 2010, 140(3): 446–453.CrossRefPubMedPubMedCentralGoogle Scholar
  2. [2]
    Yang C S, Wang H, Li G X, et al. Cancer prevention by tea: Evidence from laboratory studies[J]. Pharmacological Research, 2011, 64(2): 113–122.CrossRefPubMedGoogle Scholar
  3. [3]
    Recio M C, Andujar I, Rios J L. Anti-inflammatory agents from plants: Progress and potential[J]. Current Medicinal Chemistry, 2012, 19(14): 2088–2103.CrossRefPubMedGoogle Scholar
  4. [4]
    Deng Y T, Lin J K. EGCG inhibits the invasion of highly invasive CL1-5 lung cancer cells through suppressing MMP-2 expression via JNK signaling and induces G2/M arrest[ J]. J Agric Food Chem, 2011, 59(24): 13318–13327.CrossRefPubMedGoogle Scholar
  5. [5]
    Man G C W, Wang C C, Xu H, et al. Green tea epigallocatechin-3-gallate inhibits angiogenesis and suppresses vascular endothelial growth factor C/vascular endothelial growth factor receptor 2 expression and signaling in experimental endometriosis in vivo [J]. Fertility & Sterility, 2011, 96(4): 1021–1028.CrossRefGoogle Scholar
  6. [6]
    Shimizu M, Shirakami Y, Sakai H, et al. (−)-Epigallocatechin gallate inhibits growth and activation of the VEGF/ VEGFR axis in human colorectal cancer cells[J]. Chemico-Biological Interactions, 2010, 185(3): 247–252.CrossRefPubMedGoogle Scholar
  7. [7]
    Zhu B H, Chen H Y, Zhan W H, et al. (−)-Epigallocatechin-3-gallate inhibits VEGF expression induced by IL-6 via Stat3 in gastric cancer[J]. World Journal of Gastroenterology, 2011, 17(18): 2315–2325.CrossRefPubMedPubMedCentralGoogle Scholar
  8. [8]
    Tudoran O, Soritau O, Balacescu O, et al. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells[J]. Journal of Cellular & Molecular Medicine, 2012, 16(3): 520–530.CrossRefGoogle Scholar
  9. [9]
    Hoan N N, Jun P H, Yang S S, et al. Anti-cancer efficacy of nonthermal plasma dissolved in a liquid, liquid plasma in heterogeneous cancer cells[J]. Scientific Reports, 2016, 6: 29020.CrossRefGoogle Scholar
  10. [10]
    Yang J, Wei D, Liu J. Repressions of MMP-9 expression and NF-kappa B localization are involved in inhibition of lung carcinoma 95-D cell invasion by (−)-epigallocatechin-3-gallate [J]. Biomedicine & Pharmacotherapy, 2005, 59(3): 98–103.CrossRefGoogle Scholar
  11. [11]
    Forester S C, Lambert J D. Synergistic inhibition of lung cancer cell lines by (−)-epigallocatechin-3-gallate in combination with clinically used nitrocatechol inhibitors of catechol-O-methyltransferase [J]. Carcinogenesis, 2014, 35(2): 365–372.CrossRefPubMedGoogle Scholar
  12. [12]
    Zhao B L. Oxygen Free Radical and Nature Antioxidant[M]. Beijing: Science Press, 1999(Ch).Google Scholar
  13. [13]
    Giancotti F G, Ruoslahti E. Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells[J]. Cell, 1990, 60(5): 849–859.CrossRefPubMedGoogle Scholar
  14. [14]
    Liu J W, Nagao N, Kageyama K, et al. Antimetastatic and anti-invasive ability of phospho-ascorbyl palmitate through intracellular ascorbate enrichment and the resultant antioxidant action[J]. Oncology Research, 1999, 11(10): 479–487.PubMedGoogle Scholar
  15. [15]
    Zhang G, Miura Y, Yagasaki K. Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture[J]. Cytotechnology, 1999, 31(1-2): 37–44.CrossRefPubMedPubMedCentralGoogle Scholar
  16. [16]
    Aykinburns N, Ahmad I M, Zhu Y, et al. Increased levels of superoxide and H2O2 mediate the differential susceptibility of cancer cells versus normal cells to glucose deprivation[J]. Biochemical Journal, 2009, 418(1): 29–37.CrossRefGoogle Scholar
  17. [17]
    Su D F, Xu L P, Miao C Y, et al. Two useful methods for evaluating antihypertensive drugs in conscious freely moving rats[J]. Acta Pharmacologica Sinica, 2004, 25(2): 148–151.PubMedGoogle Scholar
  18. [18]
    Gao Y, Li W, Jia L, et al. Enhancement of (-)-epigallocatechin-3-gallate and theaflavin-3-3'-digallate induced apoptosis by ascorbic acid in human lung adenocarcinoma SPC-A-1 cells and esophageal carcinoma Eca-109 cells via MAPK pathways.[J]. Biochemical & Biophysical Research Communications, 2013, 438(2): 370–374.CrossRefGoogle Scholar
  19. [19]
    Price J E. Clonogenicity and experimental metastatic potential of spontaneous mouse mammary neoplasms[J]. J Natl Cancer Inst, 1986, 77(2): 529–535.PubMedGoogle Scholar
  20. [20]
    López-Lázaro M. Dual role of hydrogen peroxide in cancer: Possible relevance to cancer chemoprevention and therapy[J]. Cancer Letters, 2007, 252(1): 1–8.CrossRefPubMedGoogle Scholar
  21. [21]
    Liou G Y, Storz P. Reactive oxygen species in cancer[J]. Free Radical Research, 2010, 44(5): 479–496.CrossRefPubMedGoogle Scholar
  22. [22]
    Deepagan V G, Kwon S, You D G, et al. In situ diselenide-crosslinked polymeric micelles for ROS-mediated anticancer drug delivery[J]. Biomaterials, 2016, 103: 56–66.CrossRefPubMedGoogle Scholar
  23. [23]
    Nonaka Y, Iwagaki H, Kimura T, et al. Effect of reactive oxygen intermediates on the in vitro invasive capacity of tumor cells and liver metastasis in mice[J]. International Journal of Cancer Journal International Du Cancer, 1993, 54(6): 983–986.CrossRefPubMedGoogle Scholar
  24. [24]
    Binker M G, Binker-Cosen A A, Richards D, et al. EGF promotes invasion by PANC-1 cells through Rac1/ROS-dependent secretion and activation of MMP-2[J]. Biochemical & Biophysical Research Communications, 2009, 379(2): 445–450.CrossRefGoogle Scholar
  25. [25]
    Lee K H, Sang W K, Kim J R. Reactive oxygen species regulate urokinase plasminogen activator expression and cell invasion via mitogen-activated protein kinase pathways after treatment with hepatocyte growth factor in stomach cancer cells[J]. Journal of Experimental & Clinical Cancer Research: CR, 2009, 28(1): 73.CrossRefPubMedCentralGoogle Scholar
  26. [26]
    Salim A S. Oxygen-derived free-radical scavengers prolong survival in gastric cancer. Chemotherapy, 1992, 38: 135–144.CrossRefPubMedGoogle Scholar
  27. [27]
    Anasagasti M J, Martin J J, Mendoza L, et al. Glutathione protests metastatic melanoma cells against oxidative stress in the murine hepatic microvasculature. Hepatology, 1998, 27: 1249–1256.CrossRefPubMedGoogle Scholar
  28. [28]
    Albini A, D'Agostini F, Giunciuglio D, et al. Inhibition of invasion, gelatinase activity, tumor take and metastasis of malignant cells by N-acetylcysteine.[J]. International Journal of Cancer, 1995, 61(1): 121–129.CrossRefPubMedGoogle Scholar
  29. [29]
    Gohji K, Nakajima M, Boyd D, et al. Organ-site dependence for the production of urokinase-type plasminogen activator and metastasis by human renal cell carcinoma cells[J]. American Journal of Pathology, 1997, 151(6): 1655–1661.PubMedPubMedCentralGoogle Scholar
  30. [30]
    Kim A, Kim M J, Yang Y, et al. Suppression of NF-kappaB activity by NDRG2 expression attenuates the invasive potential of highly malignant tumor cells[J]. Carcinogenesis, 2009, 30(6): 927–936.CrossRefPubMedGoogle Scholar
  31. [31]
    Xiang W, Yu Z, Yan H, et al. Isothiocyanates induce oxidative stress and suppress the metastasis potential of human non-small cell lung cancer cells[J]. Bmc Cancer, 2010, 10(1): 269.CrossRefGoogle Scholar
  32. [32]
    Lai W W, Hsu S C, Chueh F S, et al. Quercetin inhibits migration and invasion of SAS human oral cancer cells through inhibition of NF-κB and matrix metalloproteinase-2/-9 signaling pathways[J]. Anticancer Research, 2013, 33(5): 1941–1950.PubMedGoogle Scholar
  33. [33]
    Cullen S, Ponnappan S, Ponnappan U. Redox-regulated pathway of tyrosine phosphorylation underlies NF-κB induction by an atypical pathway independent of the 26S proteasome[ J]. Biomolecules, 2015, 5(1): 95–112.CrossRefPubMedPubMedCentralGoogle Scholar
  34. [34]
    Lee J Y, Paik J S, Yun M, et al. The effect of (-)-epigallocatechin-3-gallate on IL-1β induced IL-8 expression in orbital fibroblast from patients with thyroid-associated ophthalmopathy[J]. Plos One, 2016, 11(2): e0148645.CrossRefPubMedPubMedCentralGoogle Scholar
  35. [35]
    Yang F, Oz H S, Barve S, et al. The green tea polyphenol (-)-epigallocatechin-3-gallate blocks nuclear factor-kappa B activation by inhibiting I kappa B kinase activity in the intestinal epithelial cell line IEC-6[J]. Molecular Pharmacology, 2001, 120(5): 528–533.Google Scholar
  36. [36]
    Cheng G, Yu W H, Yan C, et al. Nuclear factor-κB is involved in oxyhemoglobin-induced endothelin-1 expression in cerebrovascular muscle cells of the rabbit basilar artery[J]. Neuroreport, 2016, 27(12): 875–882.CrossRefPubMedGoogle Scholar
  37. [37]
    Panicker S R, Sreenivas P, Babu M S, et al. Quercetin attenuates monocyte chemoattractant protein-1 gene expression in glucose primed aortic endothelial cells through NF-κB and AP-1[J]. Pharmacological Research, 2010, 62(4): 328–336.CrossRefPubMedGoogle Scholar
  38. [38]
    Calfee-Mason K G, Lee E Y, Spear B T, et al. Role of the p50 subunit of NF-kappaB in vitamin E-induced changes in mice treated with the peroxisome proliferator, ciprofibrate[J]. Food & Chemical Toxicology, 2008, 46(6): 2062–2073.CrossRefGoogle Scholar

Copyright information

© Wuhan University and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Food Science and TechnologyShanghai Ocean UniversityShanghaiChina
  2. 2.Shanghai Engineering Research Center of Aquatic-Product Processing & PreservationShanghai Ocean UniversityShanghaiChina
  3. 3.Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai)Ministry of AgricultureShanghaiChina
  4. 4.College of Aqua-Life Science and TechnologyShanghai Ocean UniversityShanghaiChina

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