Wuhan University Journal of Natural Sciences

, Volume 20, Issue 5, pp 413–420 | Cite as

Histopathology and ultrastructural pathology of cyprinid herpesvirus II (CyHV-2) infection in gibel carp, Carassius auratus gibelio

  • Nan Jiang
  • Jin Xu
  • Jie Ma
  • Yuding Fan
  • Yong Zhou
  • Wenzhi Liu
  • Lingbing Zeng
Biology and Enviromental Science

Abstract

Cyprinid herpesvirus II (CyHV-2) infection is identified in cultured gibel carp, Carassius auratus gibelio, with high mortality in China in recent years. Histological pathology includes acute hepatocellular necrosis, splenic necrosis, kidney necrosis, hyperplasia of the secondary lamellae with focal necrosis. Acute necrotic myocarditis and granulocytes are prominent within the cardiac lumen in infected fish. In addition, necrosis is observed in the submucosa and mucosa epithelium of intestinal tract. Edemas are observed in renal glomerulus, submucosa and mucosa epithelium of intestinal tract, myocardial cells and neurons. Transmission electron microscopy indicates the cytoplasmic inclusions in splenocytes, glomerulus cells and hematopoietic tissue cells of kidney, epithelial cells of gills and brain cells. The histopathology and ultrastructural pathology in CyHV-2 infected gibel carp are characterized with extensive necrosis and cytoplasmic inclusions in spleen, kidney, gill and brain, which suggests that CyHV-2 may mainly infect the spleen, kidney, gill and brain of fish.

Keywords

Cyprinid herpesvirus II gibel carp histopathology ultrastructural pathology necrosis 

CLC number

S941.41+4 

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References

  1. [1]
    Waltzek T B, Kelley G O, Stone D M, et al. Koi herpesvirus represents a third cyprinid herpesvirus (CyHV-3) in the family Herpesviridae [J]. Journal of General Virology, 2005, 86(6): 1659–1667.CrossRefPubMedGoogle Scholar
  2. [2]
    Davison A J, Eberle R, Ehlers B, et al. The order Herpesvirales [J]. Arch Ives of Virology, 2009, 154(1): 171–177.CrossRefGoogle Scholar
  3. [3]
    Hanson L, Dishon A, Kotler M. Herpesviruses that infect fish [J]. Viruses, 2011, 3(11): 2160–2191.PubMedCentralCrossRefPubMedGoogle Scholar
  4. [4]
    Jung S J, Miyazaki T. Herpesviral haematopoietic necrosis of goldfish, Carassius auratus L. [J]. Journal of Fish Disease, 1995, 18(3): 211–220.CrossRefGoogle Scholar
  5. [5]
    Groff J M, LaPatra S E, Munn R J, et al. A viral epizootic in cultured populations of juvenile goldfish due to a putative herpesvirus etiology [J]. Journal of Veterinary Diagnostic Investigation, 1998, 10(4): 375–378.CrossRefPubMedGoogle Scholar
  6. [6]
    Chang P H, Lee S H, Chang H C, et al. Epizootic of herpes-like virus infection in goldfish, Carassius auratus, in Taiwan [J]. Fish Pathology, 1999, 34(4): 209–210.CrossRefGoogle Scholar
  7. [7]
    Stephens F J, Raidal S R, Jones B. Haematopoietic necrosis in a goldfish (Carassius auratus) associated with an agent morphologically similar to herpesvirus[J]. Australian Veterinary Journal, 2004, 82(3): 167–169.CrossRefPubMedGoogle Scholar
  8. [8]
    Goodwin A E, Khoo L, Lapatra S E, et al. Goldfish haematopoietic necrosis herpesvirus (cyprinid herpesvirus 2) in the USA: Molecular confirmation of isolates from diseased fish [J]. Journal of Aquatic Animal Health, 2006, 18(1): 11–18.CrossRefGoogle Scholar
  9. [9]
    Philbey A W. Herpesvirus haematopoietic necrosis in a goldfish (Carassius auratus) in the UK [J]. Veterinary Record, 2006, 158(23): 800–801.CrossRefPubMedGoogle Scholar
  10. [10]
    Hine P M, Tham K M, Morrison R. Cyprinid herpesvirus 2 in New Zealand goldfish [J]. Surveillance, 2006, 33(4): 3–5.Google Scholar
  11. [11]
    Jeffery K R, Bateman K, Bayley A, et al. Isolation of a cyprinid herpesvirus 2 from goldfish, Carassius auratus L., in the UK [J]. Journal of Fish Disease, 2007, 30(11): 649–656.CrossRefGoogle Scholar
  12. [12]
    Perelberg A, Smirov M, Hutoran M, et al. Epidemiological description of a new viral disease afflicting cultured Cyprinus carpio in Israel [J]. Isr J Aquacult Bamidgeh, 2003, 55(1): 5–12.Google Scholar
  13. [13]
    Miyazaki T, Kuzuya Y, Yasumoto S, et al. Histopathological and ultrastuctural features of Koi herpesvirus (KHV)- infected carp Cyprius carpio, and the morphology and morphogenesis of KHV [J]. Disease of Aquativ Organ, 2008, 80(1): 1–11.CrossRefGoogle Scholar
  14. [14]
    EI-Din M M M. Histopathological studies in experimentally infected koi carp (Cyprius carpio Koi) with koi herpesvirus in Japan [J]. World Journal of Fish and Marine Science, 2011, 3(3): 252–259.Google Scholar
  15. [15]
    Doszpoly A, Benko M, Csaba C, et al. Introduction of the family Alloherpesviridae: The first molecular detection of herpesviruses of cyprinid fish in Hungary [J]. Magyar Allatorvosok Lapja, 2011, 133(3): 174–181.Google Scholar
  16. [16]
    Xu J, Zeng L B, Zhang H, et al. Cyprinid herpesvirus 2 infection emerged in cultured giel carp Carassius auratus in China [J]. Veterinary Microbiology, 2013, 166(3): 138–144.CrossRefPubMedGoogle Scholar
  17. [17]
    Xue M, Cui Y. Effect of several feeding stimulants on diet preference by juvenile gibel carp (Carassius auratus gibelio), fed diets with or without partial replacement of fish meal by meat and bone meal [J]. Aquaculture, 2001, 198(3): 281–292.CrossRefGoogle Scholar
  18. [18]
    Wu T, Ding Z, Ren M, et al. The histo- and ultrapathological studies on a fatal disease of Prussian carp (Carassius gibelio) in mainland China associated with cyprinid herpesvirus 2 (CyHV-2) [J]. Aquaculture, 2013, 412: 8–13.CrossRefGoogle Scholar
  19. [19]
    Miwa S, Ito T, Sano M. Morphogenesis of koi herpesvirus observed by electron microscopy [J]. Journal of Fish Disease, 2007, 30(12): 715–722.CrossRefGoogle Scholar
  20. [20]
    Goodwin A E, Merry G E, Sadler J. Detection of the herpesviral hematopoietic necrosis disease agent (cyprinid herpesvirus 2) in moribund and healthy goldfish: Validation of a quantitative PCR diagnostic method [J]. Disease of Aquatic Organisms, 2006, 69(2): 137–143.CrossRefGoogle Scholar

Copyright information

© Wuhan University and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nan Jiang
    • 1
    • 2
  • Jin Xu
    • 1
  • Jie Ma
    • 1
  • Yuding Fan
    • 1
  • Yong Zhou
    • 1
  • Wenzhi Liu
    • 1
  • Lingbing Zeng
    • 1
  1. 1.Division of Fish Disease, Yangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhan, HubeiChina
  2. 2.College of FisheriesHuazhong Agricultural UniversityWuhan, HubeiChina

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