Abstract
A slight halophilic heterotrophic nitrobacteria named gs1 was separated from the matured activated sludge. According to the morphological observation, physiological biochemical tests and sequence analysis of the 16S rDNA, strain gs1 was identified to be as Pseudomonas sp. Sodium acetate and ammonium chloride were used as carbon and nitrogen sources, respectively, to investigate the characteristics of the bacterium. When cultured for 24 h under aerobic conditions, with the removal rates of the NH4 +-N and COD being 82.2% and 74.73%, respectively, strain gs1 will have a nitrification function of producing NO2 −-N. When cultured for 24 h under aerobic conditions in nitrite medium, the removal rate of the NO2 −-N became 100%, and when cultured for 24 h under aerobic conditions in nitrate medium, the removal rate of the NO3 −-N became 97%. The result shows that this strain functions for either nitrification or denitrification, i.e., it can complete the full process of biological deoxidation.
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Papen H, von Berg R, Hinkel I, et al. Heterotrophic nitrification by alcaligenes faecalis, NO2, NO3, N2O and NO production in exponentially growing cultures[J]. Appl Environ Microbiol, 1989, 55: 2068–2072.
Lin Yan, He Yiliang, Kong Hainan, et al. Isolation and characterization of heterotrophic nitrifying bacteria in MBR[J]. Joumal of Environmental Science, 2005, 17(4): 589–592.
He Jihuan, Yu Yanping, Yu Jianyong, et al. A nonlinear dynamic model for two-strand yarn Spring[J]. Textile Res J, 2005, 75(1): 87.
Castignetti D, Hollocher T C. Heterotrophic nitrification among denitrifiers[J]. Appl Envi Microbiol, 1984, 12: 16–20.
Robertson L A, van Niel E W, Torremans R A M, et al. Simultaneous nitrification and denitrification in aerobic chemostat of Thiosphaera pantotropha[J]. Appl Envi Microbiol, 1988, 54: 2812–2818.
Arts P A M, Robertson L A, Kuenen J G. Nitrification and denitrification by Thiosphaera inaerobic chemostat cultures[J]. FEMS Microbiol Ecol, 1995, 18: 305–316.
Liu Wenyan, Yu Yanping, Hu Jihuan, et al. Effect of tension compensator on Sirofil Yarn properties[J]. Textile Research Journal, 2007, 77(4): 195–199.
Liu Wenyan, Yu Yanping, Hu Jihuan, et al. Effect of standspacing between roving and filament on sirofil yarn properties[ J]. Textile Research Journal, 2007, 77(4): 200–204.
Su Junfeng, Ma Fang, Wang Hongyu, et al. Isolation and characteristics of new heterotrophic nitrifying bacteria [J]. Journal of Donghua University (English Editon), 2007, 24(4): 546–553.
Fine D H, Lieb D, Rufeh F. Principle of operation of the thermal energy analyzer for the trace analysis of volatile and non-volatile N-nitroso compounds[J]. Journal of Chromatography A, 1975, 107(2): 351–357.
Dong Xiuzhu, Cai Miaoying. Manual of Determinative and Systematic Bacteriology[M]. Bejing: Science Press, 2001: 166–168(Ch).
Buchanan R E, Gibbons N E. Bergey’s Manual of Determinative Bacteriology[M]. 8th Edition. Translated by Institute of Microbiology Chinese Academy of Sciences. Bejing: Science Press, 1984: 12(Ch).
Robertson L A, Kuenen J G, Kleijntjens R. Aerobic denitrification and heterotrophic nitrification by Thiosphaera pantotropha[J]. Antoine Van Leeuwenhoek, 1985, 51(4): 445.
Frette L, Gejlsbjerg B, Weatermann P. Aerobic denitrifiers isolated from an alternating active sludge system[J]. FEMS Microbiol Ecol, 1997, 24: 363–370.
Brierley E D R, Wood M. Heterotrophic nitrification in an acid soil: Isolation and characterization of a nitrifying bacterium[ J]. Soil Biol Biochem, 2001, 33: 1403–1409.
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Foundation item: Supported by the National Natural Science Foundation of China (50678085, 50878107), the Innovative Programs Foundation of Graduate Education in Shandong Province, China (SDYY07091) and the Cultivate Project of Excellent Graduate Students’ Thesis of Qingdao University, China (YSPY2009014)
Biography: GUO Shasha, female, Master candidate, research direction: environmental microbiology.
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Guo, S., Zhang, P., Yu, D. et al. Screening and denitrification characteristics of a slight halophilic heterotrophic nitrobacteria. Wuhan Univ. J. Nat. Sci. 15, 363–368 (2010). https://doi.org/10.1007/s11859-010-0667-x
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DOI: https://doi.org/10.1007/s11859-010-0667-x