Abstract
This article discusses the characteristics of the drug-resistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833(PSC833) and Verapamil (Ver) upon HeLa/MMC. Relative content of cell DNA detection, H3-TdR incorporation test and drug-resistance have been experimented, meanwhile a tumor transplant model in the nude mice for Hela/MMC has been established. Then the volume of cancer, tumor weight, curve line of growing up in tumor-bearing nude mouse and its morphological variation of carcinoma organism are observed to evaluate the reversal effect of PSC833 and VER. It has been found that: ➀ Compared with HeLa cells,subline HeLa/ MMC Cells’ drug-resistant capability against Mitomycin(MMC) or cisplatin(DDP) is as 5.02 folds or 2 folds as that of the former, respectively. The Hela/MMC cell line has characteristics of multi-drug resistance and stronger multiplication; ➁ PSC833 and VER can reverse resistance of HeLa/MMC to Mitomycin in vivo.PSC833 will be a better candidate for reversing multidrug resistant than verapamil in clinic application.
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Foundation item: Supported by the Key Scientific and Technical Research Project of Hubei Provicial Department of Education (EK980038)
Biography: CHEN Hong(1965–), female, Associate professor, research direction: gynecological tumor.
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Chen, H., Chen, H., Wu, X. et al. Reversal efficacy of SDZ-PSC833 and verapamil on drug-resistant human cervical carcinoma cells line. Wuhan Univ. J. of Nat. Sci. 12, 379–384 (2007). https://doi.org/10.1007/s11859-006-0065-6
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DOI: https://doi.org/10.1007/s11859-006-0065-6