Abstract
Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA (RAPD) marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ± 0.033 and the single-locus outcrossing rate was 0.795 ± 0.032. Multilocus estimates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was −0.142 ±0.000. The estimates of multilocus outcrossing rates (t m) and single-locus outcrossing rates (t s) obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of t m.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gituru W R, Wang Q F, Wang Y, et al. Pollination Ecology, Breeding System, and Conservation of Caldesia grandis (Alismataceae), an Endangered Marsh Plant in China [J]. Bot Bull Acad Sin, 2002, 43: 231–240.
Cook C D K. Aquatic and Wetland Plants of India: A Reference Book and Identification Manual for the Vascular Plants Found in Permanent or Seasonal Fresh Water in the Subcontinent of India South of the Himalaya [M]. Oxford: Oxford University Press, 1996.
Morgante M, Vendramin G G, Olivier A M. Mating System Analysis in Pinus leucodermis Ant.: Detection of Self-fertilization in Natural Populations [J]. Heredity, 1991, 67: 197–203.
Muona V. Population Genetics in Forest Tree Improvement [M]//Brown A D H, Clegg M T, Kahler A L, et al. Plant Population Genetics Breeding and Genetic Resources. Sunderland: Sinauer, 1989: 282–292.
Ge Jingping, Lin Peng. Mating System of Bruguiera gymnorrhiza Populations of Mangrove, China [J]. Acta Phytoecologica Sin, 2001, 25: 50–56 (Ch).
He Yaping, Liu Jianquan. A review on Recent Advances in the Studies of Plant Breeding System [J]. Acta Phytoecologica Sin, 2003, 27: 151–163 (Ch).
Sun M, Ritland K. Mating System of Yellow Starthistle (Centaurea solstitialis), a Successful Colonizer in North America [J]. Heredity, 1998, 80: 225–232.
Sun M, Wong K C, Lee J S Y. Reproductive Biology and Population Genetics of a Viviparous Mangrove Species, Kandelia candel (Rhizophoraceae) [J]. Amer J Bot, 1998, 85: 1631–1637.
Wang Xiaofan, Chen Jiakuan. Quantitative Estimates of Outcrossing Rates in Natural Populations of Sagittaria trifolia Linn [J]. Hereditas, 2000, 22: 316–318 (Ch).
Barrett S C H, Eckert C G, Husband B C. Evolution Processes in Aquatic Plant Population [J]. Aquat Bot, 1993, 44: 105–145.
Fritsch P, Rieseberg L H. High Outcrossing Rates Maintain Male and Hermaphrodite Individuals in Populations of the Flowering Plant Datisca glomerata [J]. Nature, 1992, 359: 633–636.
Gaiotto F A, Bramucci M, Grattapaglia D. Estimation of Outcrossing Rate in a Breeding Population of Eucalyptus urophylla with Dominant RAPD and AFLP Markers [J]. Theor Appl Genet, 1997, 95: 842–849.
Ge X J, Sun M. Reproductive Biology and Genetic Diversity of a Cryptoviviparous Mangrove Aegiceras Corniculatum (Myrsinaceae) Using Allozyme and Inter-Simple Sequence Repeat (ISSR) Analysis [J]. Mol Ecol, 1999, 8: 2061–2069.
Maguire T L, Peakall R, Saenger P. Comparative Analysis of Genetic Diversity in the Mangrove Species Avicennia marina (Forsk.) Vierh (Avicenniaceae) Detected by AFLPs and SSRs [J]. Theor Appl Genet, 2002, 104: 388–398.
Chen Jinming, Wang Qingfeng. Genetic Diversity and Structure in a Natural Caldesia grandis Population [J]. Biodiversity Sciences, 2005, 13(5): 398–406 (Ch).
Chen J M, Wang J Y, Liu X, et al. RAPD Analysis for Genetic Variation within the Endangered Quillwort Isoetes hypsophila (Isoetaceae) [J]. Wuhan University Journal of Natural Sciences, 2005, 10: 455–459.
Ritand K, Jain S. A Model for the Estimation of Outcrossing Rate and Gene Frequencies Using n Independent Loci [J]. Heredity, 1981, 47: 35–52.
Ritland K. A Series of FORTRAN Computer Programs for Estimating Plant Mating Systems [J]. J Heredity, 1990, 81: 235.
Heun M, Murphy J P, Philips T D. A Comparison of RAPD and Isozyme Analysis for Determining the Genetic Relationships among Avena sterilis L. Accessions [J]. Theor Appl Genet, 1994, 87: 689–696.
Huff D R, Peakall R, Smouse P E. RAPD Variation within and among Natural Populations of Outcrossing Buffalograss [Buchloe dactyloides (Nutt.)] [J]. Theor Appl Genet, 1993, 86: 927–934.
Wang Xiaofan, Chen Jiakuan. Studies on Mating System of Ranalisma rostratum (Alismataceae) [J]. Acta Bot Yunnanica, 1998, 20: 315–320 (Ch).
Wang Xiaofan, Chen Jiakuan. Pollination Mechanism and Mating System of Sagittaria pygmaea (Alismataceae) [J]. Acta Bot Yunnanica, 1999, 21: 225–231 (Ch).
Wang Xiaofan, Chen Jiakuan. Floral Expression, Pollination Mechanism and Mating System of Sagittaria potamogetifolia [J]. Acta Phytoecologica Sin, 2001, 25: 155–160 (Ch).
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: Supported by the State Key Basic Research and Development Plan (G2000046805) and the National Natural Science Foundation of China (30370098, 30570111)
Biography: CHEN Jinming (1975–), male, Ph.D., research direction: plant systematics and evolutionary biology.
Rights and permissions
About this article
Cite this article
Chen, J., Gituru, W.R. & Wang, Q. Quantitative estimates of outcrossing rates in a natural population of Caldesia grandis (Alismataceae). Wuhan Univ. J. of Nat. Sci. 12, 337–342 (2007). https://doi.org/10.1007/s11859-006-0004-6
Received:
Issue Date:
DOI: https://doi.org/10.1007/s11859-006-0004-6