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NR_103776.1 as a novel diagnostic biomarker for systemic lupus erythematosus

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Abstract

Background

With the development of sequencing technologies, there is increasing evidence that long noncoding RNAs (lncRNAs) are involved in systemic lupus erythematosus (SLE). The level of NR_103776.1 expression in SLE and its clinical associations are still not well defined.

Objective

To identify differentially expressed lncRNAs and explore their functional roles in SLE.

Methods

Transcriptome sequencing was used to screen differentially expressed lncRNAs and mRNAs. Expression validation of clinical samples was performed by QRT-PCR. Bioinformatics was used to analyze its prognostic value and potential function.

Results

Of the 231 significantly differentially expressed lncRNAs, NR_103776.1 could be used to distinguish not only SLE patients and rheumatoid arthritis patients but also active SLE patients, stable SLE patients, and healthy controls. NR_103776.1 was significantly and negatively correlated with inflammatory indexes (CRP and ESR). NR_103776.1 dysregulation might contribute to the metabolism of RNA and proteins in SLE patients.

Conclusions

This study not only provided a transcriptome profile of lncRNAs aberrantly expressed in individual nucleated cells of SLE patients but also suggested NR_103776.1 as a novel potential diagnostic biomarker.

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Data availability

All data supporting the findings of this study are available within the paper and its Supplementary Information.

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Acknowledgements

We acknowledge Rheumatology and Immunology department, Affiliated Hospital of Weifang Medical University, Medicine Science and Technology Plan Project of Weifang, China (item no. WFZYY2022-4-026) and Shandong Provincial Natural Science Foundation, China (no: ZR2017LH039).

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Authors and Affiliations

  1. Department of Rheumatology and Immunology, School of Clinical Medicine, Affiliated Hospital of Weifang Medical University, Weifang Medical University, Weifang, Shandong Province, China

    Yuqun Wang, Jia He, Honglei Ma, Junhong Liu & Linping Du

  2. Department of Rheumatology and Immunology, Affiliated Hospital of Weifang Medical University, Weifang, Shandong Province, China

    Chunxiang Chai, Yajing Liu & Xiaodong Wang

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  1. Yuqun Wang
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  2. Jia He
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  3. Honglei Ma
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  4. Junhong Liu
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  6. Chunxiang Chai
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  7. Yajing Liu
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  8. Xiaodong Wang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Wang Yuqun, He Ja, Ma Honglei, Liu Junhong, Du Linping, Chai Chunxiang, Liu Yajing, and Wang Xiaodong. The first draft of the manuscript was written by Wang Yuqun and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Xiaodong Wang.

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Ethical approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of the Affiliated Hospital of Weifang Medical University (no: wyfy-2023-ky-033).

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Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare no competing interests.

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Wang, Y., He, J., Ma, H. et al. NR_103776.1 as a novel diagnostic biomarker for systemic lupus erythematosus. Ir J Med Sci 193, 211–221 (2024). https://doi.org/10.1007/s11845-023-03420-8

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  • DOI: https://doi.org/10.1007/s11845-023-03420-8

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