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Evaluation of IL-10, MCP-1, IFN gamma, and protectin D1 levels in patients with Hashimoto’s thyroiditis

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Abstract

Purpose

Hashimoto’s thyroiditis (HT) is one of the most prevalent autoimmune endocrine diseases and caused by the loss of immune tolerance for the thyroid gland. Many pathophysiological mechanisms were speculated about the development of HT. In our study, we aimed to reveal the relationship between HT and IL-10, MCP-1, IFNɤ, and PD1 levels and compare them with control subjects.

Methods

We collected 37 patients with HT and 25 controls referred to our outpatient clinic. The diagnosis of HT was based on the detection of circulating antibodies to thyroid antigens and decreasing echogenicity on thyroid USG in patients with appropriate clinical characteristics. Serum IL-10, MCP-1, IFNɤ, and PD1 levels were detected using an ELISA KIT (96 T) method according to the manufacturer’s instructions.

Results

All subjects were euthyroid (median TSH level was 1.68 mU/L in HT vs 1.83 mU/L in the controls, p = 0.672). Twenty-three of 37 patients with HT were taking L-thyroxin replacement. Levels of serum IL-10, IFNɤ, and PD1 in patients with HT were higher than the controls, but the differences were not statistically significant (p = 0.393, p = 0.495, and p = 0.052 respectively). The serum levels of MCP-1 in HT patients were statistically different and higher than the controls (p = 0.018). Correlation analysis displayed significant associations between IL-10, MCP-1, IFNɤ, and PD1 levels.

Conclusion

Our study demonstrated that serum MCP-1 levels in HT patients were significantly increased; on the other hand, significant difference was not found between HT patients and the controls in terms of serum IL-10, IFNɤ, and PD1 levels.

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Acknowledgements

We are grateful to Ahmet Topacoglu for his linguistic revision.

Funding

This project was supported by Ege University Scientific Research Projects Coordination Unit through Project Number: TGA-2022-23376.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Ege University, İzmir, 3500, Turkey

    Hatice Ozisik, Gokhan Ozgen & Mehmet Erdogan

  2. Department of Medical Biology, Ege University, İzmir, Turkey

    Ayse Cekin, Buket Ozel & Nur Selvi Gunel

  3. Department of Biostatistics and Medical Informatics, Ege University, İzmir, Turkey

    Aslı Suner

  4. Department of Medical Biochemistry, Ege University, İzmir, Turkey

    Burak Durmaz

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  1. Hatice Ozisik
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  2. Ayse Cekin
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Contributions

Conceptualization: HO, ME,NSG. Data preparation/acquisition: HO, AC, BO. Methodology: BD, AC, HO. Statistical analysis: AS Writing—original draft preparation: HO, AS, BD. Supervision: NSG, GO, ME

Corresponding author

Correspondence to Hatice Ozisik.

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The study protocol was approved by the Ethics Committee of Ege University Faculty of Medicine (21-9 T/11).

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Ozisik, H., Cekin, A., Suner, A. et al. Evaluation of IL-10, MCP-1, IFN gamma, and protectin D1 levels in patients with Hashimoto’s thyroiditis. Ir J Med Sci 192, 177–184 (2023). https://doi.org/10.1007/s11845-022-03231-3

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  • DOI: https://doi.org/10.1007/s11845-022-03231-3

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