Skip to main content

Advertisement

SpringerLink
Log in

The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis

  • Original Article
  • Published:
Irish Journal of Medical Science (1971 -) Aims and scope Submit manuscript

Abstract

Background

The first research to determine synovial fluid ADAMTS4 and serglycin levels in osteoarthritis and OA progression.

Aim

We aimed to determine ADAMTS4 and serglycin levels, interactions, and changes in the synovial fluid of knee OA, and also to determine effective in OA progression.

Methods

A case-control study was carried out including a total of 88 participants (29 patients late OA [LOA], 28 early OA [EOA], and 30 controls). Synovial fluid serglycin and ADAMTS4 levels were measured by commercially available ELISA kits, and knee functions of the patients were evaluated with The Western Ontario and McMaster Universities Osteoarthritis score (WOMAC). Logistic regression analysis was applied for the associated with progression of OA.

Results

Synovial fluid ADAMTS4 and serglycin levels were significantly higher in LOA than EOA and control groups (p < .001 and p < .001; p = .038 and p = .007, respectively). All parameters were evaluated after adjustment for age. LOA patients had significantly higher levels of WOMAC score than EOA and controls (p < .001 and p < .001). According to the logistic regression analysis, synovial fluid ADAMTS4, serglycin levels, and WOMAC score were found to be significantly associated with progression of OA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Dillon CF, Rasch EK, Gu Q et al (2006) Prevalence of knee osteoarthritis in the United States: arthritis data from thethird national health and nutrition examination survey 1991–94. J Rheumatol 33(11):2271–2279

    PubMed  Google Scholar 

  2. Maroudas A (1976) Balance between swelling pressure and collagen tension in normal and degenerate cartilage. Nature (London) 260:808–809

    Article  CAS  Google Scholar 

  3. Song RH, Tortorella MD, Malfait AM et al (2007) Aggrecan degradation in human articular cartilage explants is mediated by both ADAMTS-4 and ADAMTS-5. Arthritis Rheum 56:575–585

    Article  CAS  PubMed  Google Scholar 

  4. Arner EC (2002) Aggrecanase-mediated cartilage degradation. Curr Opin Pharmacol 2:322–329

    Article  CAS  PubMed  Google Scholar 

  5. Yaykasli KO, Hatipoglu OF, Yaykasli E et al (2015) Leptin induces ADAMTS-4, ADAMTS-5, and ADAMTS-9 genes expression by mitogen-activated protein kinases and NF-ĸB signaling pathways in human chondrocytes. Cell Biol Int 39(1):104–112. https://doi.org/10.1002/cbin.10336

    Article  CAS  PubMed  Google Scholar 

  6. Malfait AM, Liu RQ, Ijiri K, Komiya S, Tortorella MD (2002) Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage. J Biol Chem 277:22201–22208

    Article  CAS  PubMed  Google Scholar 

  7. Scully OJ, Chua PJ, Harve KS, Bay BH, Yip GW (2012) Serglycin in health and diseases. Anat Rec (Hoboken) 295(9):1415–1420. https://doi.org/10.1002/ar.22536

    Article  CAS  Google Scholar 

  8. Schick BP, Gradowski JF, San Antonio JD (2001) Synthesis, secretion, and subcellular localization of serglycin proteoglycan in human endothelial cells. Blood 97(2):449–458

    Article  CAS  PubMed  Google Scholar 

  9. Omtvedt LA, Kolset SO, Thoen J, Førre Y, Gill MR (2001) Serglycin expression in CD2+ and CD14+ cells from patients with various rheumatic diseases. Scand J Rheumatol 30(3):164–166

    Article  CAS  PubMed  Google Scholar 

  10. Zhang L, Yang M, Yang D, Cavey G, Davidson P, Gibson G (2010) Molecular interactions of MMP-13 C-terminal domain with chondrocyte proteins. Connect Tissue Res 51(3):230–239. https://doi.org/10.3109/03008200903288902

    Article  CAS  PubMed  Google Scholar 

  11. Kellgren JH, Lawrence JS (1957) Radiological assessment of osteoarthrosis. Ann Rheum Dis 16:494e502

    Google Scholar 

  12. Bellamy N, Buchanan WW, Goldsmith CH, Campbell J, Stitt LW (1988) Validation study of WOMAC: a health status instrument for measuring clinically important patient relevant outcomes to anti-rheumatic drug therapy in patients with osteoarthritis of the hip or knee. J Rheumatol 15:1833–1840

    CAS  PubMed  Google Scholar 

  13. Tüzün EH, Eker L, Aytar A, Daşkapan A, Bayramoğlu M (2005) Acceptability, reliability, validity and responsiveness of the Turkish version of WOMAC osteoarthritis index. Osteoarthr Cartil 13(1):28–33

    Article  Google Scholar 

  14. Miller RG. (1966) Simultaneous statistical inference. McGraw-Hill: New York

  15. Xue J, Wang J, Liu Q et al (2013) Tumor necrosis factor-α induces ADAMTS-4 expression in human osteoarthritis chondrocytes. Mol Med Rep 8(6):1755–1760. https://doi.org/10.3892/mmr.2013.1729

    Article  CAS  PubMed  Google Scholar 

  16. He Y, Zheng Q, Jiang M, Sun S, Christiansen TG, Kassem M, Karsdal MA, Bay-Jensen AC (2015) The effect of protease inhibitors on the induction of osteoarthritis-related biomarkers in bovine full-depth cartilage explants. PLoS One 10(4):e0122700. https://doi.org/10.1371/journal.pone.0122700

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Moulharat N, Lesur C, Thomas M, Rolland-Valognes G, Pastoureau P, Anract P, de Ceuninck F, Sabatini M (2004) Effects of transforming growth factor-beta on aggrecanase production and proteoglycan degradation by human chondrocytes in vitro. Osteoarthr Cartil 12(4):296–305

    Article  CAS  Google Scholar 

  18. Roberts S, Evans H, Wright K, van Niekerk L, Caterson B, Richardson JB, Kumar KHS, Kuiper JH (2015) ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion. Osteoarthr Cartil 23(9):1622–1626. https://doi.org/10.1016/j.joca.2015.05.006

    Article  CAS  Google Scholar 

  19. Peng S, Zheng Q, Zhang X, Dai L, Zhu J, Pi Y, Hu X, Cheng W, Zhou C, Sha Y, Ao Y (2013) Detection of ADAMTS-4 activity using a fluorogenic peptide-conjugated Au nanoparticle probe in human knee synovial fluid. ACS Appl Mater Interfaces 5(13):6089–6096. https://doi.org/10.1021/am400854z.

    Article  CAS  PubMed  Google Scholar 

  20. Zhang E, Yan X, Zhang M, Chang X, Bai Z, He Y, Yuan Z (2013) Aggrecanases in the human synovial fluid at different stages of osteoarthritis. Clin Rheumatol 32(6):797–803. https://doi.org/10.1007/s10067-013-2171-0

    Article  CAS  PubMed  Google Scholar 

  21. Kamm JL, Nixon AJ, Witte TH (2010) Cytokine and catabolic enzyme expression in synovium, synovial fluid and articular cartilage of naurally osteoarthritic equine carpi. Equine Vet J 42(8):693–699. https://doi.org/10.1111/j.2042-3306.2010.00140.x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Bau B, Gebhard PM, Haag J, Knorr T, Bartnik E, Aigner T (2002) Relative messenger RNA expression profiling of collagenases and aggrecanases in human articular chondrocytes in vivo and in vitro. Arthritis Rheum 46:2648e57

    Article  CAS  Google Scholar 

  23. Wang P, Tortorella M, England K, Malfait AM, Thomas G, Arner EC, Pei D (2004) Proprotein convertase furin interacts with and cleaves pro-ADAMTS4 (Aggrecanase-1) in the trans-Golgi network. J Biol Chem 279(15):15434–15440

    Article  CAS  PubMed  Google Scholar 

  24. Mayer G, Hamelin J, Asselin MC, Pasquato A, Marcinkiewicz E, Tang M, Tabibzadeh S, Seidah NG (2008) The regulated cell surface zymogen activation of the proprotein convertase PC5A directs the processing of its secretory substrates. J Biol Chem 283(4):2373–2384

    Article  CAS  PubMed  Google Scholar 

  25. Ariyoshi W, Takahashi N, Hida D, Knudson CB, Knudson W (2012) Mechanisms involved in enhancement of the expression and function of aggrecanases by hyaluronan oligosaccharides. Arthritis Rheum 64(1):187–197. https://doi.org/10.1002/art.33329

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Kataoka Y, Ariyoshi W, Okinaga T, Kaneuji T, Mitsugi S, Takahashi T, Nishihara T (2013) Mechanisms involved in suppression of ADAMTS4 expression in synoviocytes by high molecular weight hyaluronic acid. Biochem Biophys Res Commun 432(4):580–585. https://doi.org/10.1016/j.bbrc.2013.02.043

    Article  CAS  PubMed  Google Scholar 

  27. Erwin WM, Islam D, Inman RD, Fehlings MG, Tsui FWL (2011) Notochordal cells protect nucleus pulposus cells from degradation and apoptosis: implications for the mechanisms of intervertebral disc degeneration. Arthritis Res Ther 13(6):R215. https://doi.org/10.1186/ar3548

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Korpetinou A, Skandalis SS, Labropoulou VT, Smirlaki G, Noulas A, Karamanos NK, Theocharis AD (2014) Serglycin: at the crossroad of inflammation and malignancy. Front Oncol 3:327. https://doi.org/10.3389/fonc.2013.00327

    Article  PubMed  PubMed Central  Google Scholar 

  29. D’Ascola A, Scuruchi M, Avenoso A, Bruschetta G, Campo S, Mandraffino G, Campo GM (2018) Serglycin is involved in inflammatory response in articular mouse chondrocytes. Biochem Biophys Res Commun 499(3):506–512. https://doi.org/10.1016/j.bbrc.2018.03.178

    Article  CAS  PubMed  Google Scholar 

  30. Toyama-Sorimachi N, Kitamura F, Habuchi H, Tobita Y, Kimata K, Miyasaka M (1997) Widespread expression of chondroitin sulfate type serglycins with CD44 binding ability in hematopoietic cells. J Biol Chem 272(42):26714–26719

    Article  CAS  PubMed  Google Scholar 

  31. Lord MS, Cheng B, Farrugia BL, McCarthy S, Whitelock JM (2017) Platelet factor 4 binds to vascular proteoglycans and controls both growth factor activities and platelet activation. J Biol Chem 292(10):4054–4063

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. de Girolamo L, Kon E, Filardo G, Marmotti AG, Soler F, Peretti GM, Vannini F, Madry H, Chubinskaya S (2016) Regenerative approaches for the treatment of early OA. Knee Surg Sports Traumatol Arthrosc 24(6):1826–1835. https://doi.org/10.1007/s00167-016-4125-y

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We would like to thank patients and staff who participated in the study.

Funding

The author did not receive financial support from any institution for this study.

Author information

Authors and Affiliations

  1. Konya Beysehir State Hospital, Beyşehir Devlet Hastanesi, 042100, Konya, Turkey

    kenan ÖZLER

Authors
  1. kenan ÖZLER
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to kenan ÖZLER.

Ethics declarations

Conflict of interest

The author declares no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

ÖZLER, k. The role of increased synovial fluid A disintegrin and metalloproteinase with thrombospondin motifs4 and serglycin levels in osteoarthritis. Ir J Med Sci 188, 867–872 (2019). https://doi.org/10.1007/s11845-018-1945-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11845-018-1945-4

Keywords

Search

Navigation