Molecular hydrogen (H2) effectively treats obesity-related disorders in animal models, yet no studies have investigated the effectiveness and safety of H2 for improving biomarkers of obesity in humans.
In this double blind, placebo-controlled, crossover pilot trial, we evaluated the effects of H2 intervention on body composition, hormonal status, and mitochondrial function in ten (n = 10) middle-aged overweight women.
Volunteers received either hydrogen-generating minerals (supplying ~6 ppm of H2 per day) or placebo by oral administration of caplets for 4 weeks. The primary end-point of treatment efficacy was the change in the body fat percentage from baseline to 4 weeks. In addition, assessment of other body composition indices, screening laboratory studies, and evaluation of side effects were performed before and at follow-up. Clinical trial registration www.clinicaltrials.gov, ID number NCT02832219.
No significant differences were observed between treatment groups for changes in weight, body mass index, and body circumferences at 4-week follow-up (P > 0.05). H2 treatment significantly reduced body fat percentage (3.2 vs. 0.9%, P = 0.05) and arm fat index (9.7 vs. 6.0%, P = 0.01) compared to placebo administration, respectively. This was accompanied by a significant drop in serum triglycerides after H2 intervention comparing to placebo (21.3 vs. 6.5%; P = 0.04), while other blood lipids remained stable during the study (P > 0.05). Fasting serum insulin levels dropped by 5.4% after H2 administration, while placebo intervention augmented insulin response by 29.3% (P = 0.01).
It appears that orally administered H2 as a blend of hydrogen-generating minerals might be a beneficial agent in the management of body composition and insulin resistance in obesity.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Marseglia L, Manti S, D'Angelo G, Nicotera A, Parisi E, Di Rosa G, Gitto E, Arrigo T (2014) Oxidative stress in obesity: a critical component in human diseases. Int J Mol Sci 16:378–400
Ríos-Hoyo A, Gutiérrez-Salmeán G (2016) New dietary supplements for obesity: what we currently know. Curr Obes Rep 5:262–270
Bournat JC, Brown CW (2010) Mitochondrial dysfunction in obesity. Curr Opin Endocrinol Diabetes Obes 17:446–452
Ostojic SM (2015) Targeting molecular hydrogen to mitochondria: barriers and gateways. Pharmacol Res 94:51–53
Ohno K, Ito M, Ichihara M, Ito M (2012) Molecular hydrogen as an emerging therapeutic medical gas for neurodegenerative and other diseases. Oxid Med Cell Longev 2012:353152
Huang CS, Kawamura T, Toyoda Y, Nakao A (2010) Recent advances in hydrogen research as a therapeutic medical gas. Free Radic Res 44:971–982
Wang QJ, Zha XJ, Kang ZM, Xu MJ, Huang Q, Zou DJ (2012) Therapeutic effects of hydrogen saturated saline on rat diabetic model and insulin resistant model via reduction of oxidative stress. Chin Med J 125:1633–1637
Kamimura N, Nishimaki K, Ohsawa I, Ohta S (2011) Molecular hydrogen improves obesity and diabetes by inducing hepatic FGF21 and stimulating energy metabolism in db/db mice. Obesity 19:1396–1403
Craig CL, Marshall AL, Sjöström M, Bauman AE, Booth ML, Ainsworth BE, Pratt M, Ekelund U, Yngve A, Sallis JF, Oja P (2003) International physical activity questionnaire: 12-country reliability and validity. Med Sci Sports Exerc 35:1381–1395
Hebert JR, Ockene IS, Hurley TG, Luippold R, Well AD, Harmatz MG (1997) Development and testing of a seven-day dietary recall. Dietary assessment working group of the Worcester Area Trial for Counseling in Hyperlipidemia (WATCH). J Clin Epidemiol 50:925–937
Ostojic SM (2012) Serum alkalinization and hydrogen-rich water in healthy men. Mayo Clin Proc 87:501–503
Gibson RS (2005) Principle of nutritional assessment. Oxford University Press, Oxford
Ostojic SM (2015) Molecular hydrogen: an inert gas turns clinically effective. Ann Med 47:301–304
Kajiyama S, Hasegawa G, Asano M, Hosoda H, Fukui M, Nakamura N, Kitawaki J, Imai S, Nakano K, Ohta M, Adachi T, Obayashi H, Yoshikawa T (2008) Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose intolerance. Nutr Res 28:137–143
Nakao A, Toyoda Y, Sharma P, Evans M, Guthrie N (2010) Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome—an open label pilot study. J Clin Biochem Nutr 46:140–149
Song G, Li M, Sang H, Zhang L, Li X, Yao S, Yu Y, Zong C, Xue Y, Qin S (2013) Hydrogen-rich water decreases serum LDL-cholesterol levels and improves HDL function in patients with potential metabolic syndrome. J Lipid Res 54:1884–1893
Janssen I, Fortier A, Hudson R, Ross R (2002) Effects of an energy-restrictive diet with or without exercise on abdominal fat, intermuscular fat, and metabolic risk factors in obese women. Diabetes Care 25:431–438
Ohta S (2011) Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des 17:2241–2252
Shiiya T, Nakazato M, Mizuta M, Date Y, Mondal MS, Tanaka M, Nozoe S, Hosoda H, Kangawa K, Matsukura S (2002) Plasma ghrelin levels in lean and obese humans and the effect of glucose on ghrelin secretion. J Clin Endocrinol Metab 87:240–244
McCarty MF (2015) Potential ghrelin-mediated benefits and risks of hydrogen water. Med Hypotheses 84:350–355
Blüher M (2009) Adipose tissue dysfunction in obesity. Exp Clin Endocrinol Diabetes 117:241–250
Chen YD, Varasteh BB, Reaven GM (1993) Plasma lactate concentration in obesity and type 2 diabetes. Diabete Metab 19:348–354
Andersen LW, Mackenhauer J, Roberts JC, Berg KM, Cocchi MN, Donnino MW (2013) Etiology and therapeutic approach to elevated lactate levels. Mayo Clin Proc 88:1127–1140
Qian L, Shen J, Chuai Y, Cai J (2013) Hydrogen as a new class of radioprotective agent. Int J Biol Sci 9:887–894
This work was supported by the Serbian Ministry of Education, Science and Technological Development (grant number 175037), the Provincial Secretariat for Science and Technological Development (grant number 114-451-710), the Faculty of Sport and Physical Education, University of Novi Sad (2016 Annual Award), and SevenPoint2, Newport Beach, USA (grant number 075-B/2016). The funders had no role in the study design, data collection, analysis, and interpretation, decision to publish, or preparation of the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
Statement of human rights
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.
About this article
Cite this article
Korovljev, D., Trivic, T., Drid, P. et al. Molecular hydrogen affects body composition, metabolic profiles, and mitochondrial function in middle-aged overweight women. Ir J Med Sci 187, 85–89 (2018). https://doi.org/10.1007/s11845-017-1638-4
- Mitochondrial dysfunction
- Molecular hydrogen