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miR-210 expression in PBMCs from patients with systemic lupus erythematosus and rheumatoid arthritis

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Abstract

Background

In hypoxic conditions, miRNA-210 plays an important role in regulating the expression of hypoxia-inducing factor-1α (HIF-1α) and the differentiation of T helper 17 (Th17) cells, and this may be involved in the development and function of the immune system.

Aims

This study was to investigate the miR-210 expression levels in peripheral blood mononuclear cells (PBMCs) from patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and its association with the clinical and laboratory features of both diseases.

Methods

Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect miR-210 expression levels in PBMCs from 35 patients with SLE, 38 patients with RA, and 35 healthy controls.

Results

Compared with the healthy controls, the miR-210 expression levels were significantly increased in patients with SLE (P = 0.001) and there was increased significantly expression of miR-210 in SLE with pleuritis (Z = −2.345, P = 0.019) and anti-SSB/La-positive group (Z = −2.076, P = 0.038). However, we have not found the significant correlation between the miR-210 levels and the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) score (r s = 0.091, P = 0.602). Although, no significant difference between miR-210 levels in RA patients and those in healthy controls was found (Z = −1.226, P = 0. 220). There was a significant decreased expression of miR-210 in active RA patients than inactive RA patients (Z = −4.011, P < 0.001).

Conclusions

The dysregulation of miR-210 levels in SLE and RA patients suggests that miR-210 might play an important role in the pathogenesis of these diseases.

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Acknowledgements

Thank you to the First Affiliated Hospital of Anhui Medical University and Anhui Provincial Hospital.

Author information

Author notes

  1. Qing Huang and Shuang-Shuang Chen contributed equally to this work and should be considered as co-first authors.

Authors and Affiliations

  1. Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, 230032, People’s Republic of China

    Q. Huang, S.-S. Chen, J. Li, S.-S. Tao, M. Wang, R.-X. Leng, H.-F. Pan & D.-Q. Ye

  2. Department of Epidemiology and Biostatistics, School of Public Health, Bengbu Medical College, Bengbu, Auhui, People’s Republic of China

    Q. Huang

  3. Anhui Province Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, Hefei, Auhui, People’s Republic of China

    S.-S. Chen, J. Li, S.-S. Tao, M. Wang, R.-X. Leng, H.-F. Pan & D.-Q. Ye

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  1. Q. Huang
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Corresponding author

Correspondence to D.-Q. Ye.

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Funding

This study was supported by the National Natural Science Foundation of China (81172764).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Novelty statement

The expression of miR-210 was detected by RT-qPCR in these patients. This research is the first study to investigate the miR-210 expression levels in peripheral blood mononuclear cells (PBMCs) from patients with SLE and RA. And the results can provide an evidence for the further study of its function in the pathogenesis of SLE and RA.

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Huang, Q., Chen, SS., Li, J. et al. miR-210 expression in PBMCs from patients with systemic lupus erythematosus and rheumatoid arthritis. Ir J Med Sci 187, 243–249 (2018). https://doi.org/10.1007/s11845-017-1634-8

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  • DOI: https://doi.org/10.1007/s11845-017-1634-8

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