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Irish Journal of Medical Science (1971 -)

, Volume 187, Issue 1, pp 225–229 | Cite as

Small interfering RNA targeting receptor for advanced glycation end products protects the rats from multibacterial sepsis

  • X. Zhao
  • Y. N. Liao
  • Q. HuangEmail author
Brief Report

Abstract

Background

Sepsis is a major challenge in clinical medicine, and treatment options are limited. Recently, the receptor for advanced glycation end products (RAGE) appears to be an excellent target for new therapeutic agents.

Aims

The objective of this study is to investigate the effect of small interfering RNA (siRNA) targeting RAGE on the outcome of multibacterial sepsis induced by cecal ligation and puncture (CLP) in a rat model.

Methods

A vector-based RAGE-targeted siRNA expression system (Psilencer-siRNA) was constructed and injected into rats via the jugular vein catheter after CLP injury. The RAGE expression in livers, survival rate, and plasma cytokine levels after CLP were compared between Psilencer-siRNA treated and control rats.

Results

The expression of RAGE in livers which was upregulated after CLP injury was greatly curtailed by Psilencer-siRNA administration. Compared to control rats, the Psilencer-siRNA-treated rats had significantly higher survival rate (p < 0.05) and markedly decreased plasma cytokine levels (p < 0.001) after CLP.

Conclusions

Targeting RAGE by siRNA might attenuate hyperinflammation, improve survival rate, and offer new therapeutic options for sepsis.

Keywords

Cecal ligation and puncture Receptor for advanced glycation end products Sepsis Small interfering RNA 

Notes

Compliance with ethical standards

Funding

This study was funded by the National Natural Scientific Foundation of China (Grant number: 81370514).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Ethics Committee of Jinling Hospital, Medical School of Nanjing University and was performed in accordance with the Guide for Care and Use of Laboratory Animals from National Institutes of Health. This article did not contain any studies with human participants performed by any of the authors.

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Copyright information

© Royal Academy of Medicine in Ireland 2017

Authors and Affiliations

  1. 1.Research Institute of General SurgeryJinling Hospital, Medical School of Nanjing UniversityNanjingChina

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