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Influence of a CYP1A2 polymorphism on post-exercise heart rate variability in response to caffeine intake: a double-blind, placebo-controlled trial

Irish Journal of Medical Science (1971 -) volume 186, pages 285–291 (2017)Cite this article

Abstract

Background

Proposed differences in caffeine metabolism due to the CYP1A2*1F polymorphism have been linked to variations in cardiovascular disease risk.

Aims

We examined the influence of a CYP1A2*1F polymorphism on post-exercise heart rate variability (HRV) in response to caffeine intake.

Methods

Volunteers were identified as A/A homozygotes (A/A; 4 females and 7 males; age: 25.3 ± 4.1 years; BMI: 25.9 ± 4.4 kg/m2) or C allele carriers (C allele; 3 females and 6 males; age: 25.5 ± 2.8 years; BMI: 26.6 ± 5.0 kg/m2) for participation in a repeated measures, counterbalanced, double-blind, placebo-controlled trial. Participants chewed three pieces of gum containing either caffeine (CAF) (100 mg/piece) or placebo for 5 min. Thereafter, participants cycled for 15 min at 75 % of their peak oxygen consumption. Eight HRV indices computed during 5 min at baseline (BASE), 0–5 min after exercise (POST1), and 5–10 min after exercise (POST2) were used for analysis.

Results

No significant group differences were detected in HRV indices at BASE, POST1, or POST2 during both trials (p > 0.05). Rate of recovery (POST2–POST1) for the square root of the mean of squared differences between successive RR intervals (RMSSD) was significantly different between A/A (6.0 ± 2.5 ms) and C allele (3.6 ± 2.5 ms) groups during the CAF trial (p = 0.048).

Conclusions

Rate of RMSSD recovery was the only variable influenced by the CYP1A2*IF polymorphism during post-exercise in response to caffeine intake. Thus, the CYP1A2*1F polymorphism did not overtly influence the effects of caffeine intake on post-exercise HRV.

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References

  1. Grant DM, Tang BK, Kalow W (1983) Variability in caffeine metabolism. Clin Pharmacol Ther 33:591–602

    Article  CAS  PubMed  Google Scholar 

  2. Lelo A, Birkett DJ, Robson RA, Miners JO (1986) Comparative pharmacokinetics of caffeine and its primary demethylated metabolites paraxanthine, theobromine and theophylline in man. Br J Clin Pharmacol 22:177–182

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Perera V, Gross AS, McLachlan AJ (2012) Influence of environmental and genetic factors on CYP1A2 activity in individuals of South Asian and European ancestry. Clin Pharmacol Ther 92:511–519

    CAS  PubMed  Google Scholar 

  4. Sachse C, Brockmoller J, Bauer S, Roots I (1999) Functional significance of a C—A polymorphism in intron 1 of the cytochrome P450 CYP1A2 gene tested with caffeine. Br J Clin Pharmacol 47:445–449

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. El-Sohemy A, Cornelis MC, Kabagambe EK, Campos H (2007) Coffee, CYP1A2 genotype and risk of myocardial infarction. Genes Nutr 2:155–156

    Article  PubMed  PubMed Central  Google Scholar 

  6. Womack CJ, Saunders MJ, Bechtel MK, Bolton DJ, Martin M, Luden ND, Dunham W, Hancock M (2012) The influence of a CYP1A2 polymorphism on the ergogenic effects of caffeine. J Int Soc Sports Nutr 9:7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Palatini P, Ceolotto G, Ragazzo F, Dorigatti F, Saladini F, Papparella I, Mos L, Zanata G, Santonastaso M (2009) CYP1A2 genotype modifies the association between coffee intake and the risk of hypertension. J Hypertens 27:1594–1601

    Article  CAS  PubMed  Google Scholar 

  8. Barbieri R, Matten EC, Alabi AA, Brown EN (2005) A point-process model of human heartbeat intervals: new definitions of heart rate and heart rate variability. Am J Physiol Heart Circ Physiol 288:H424–H435

    Article  CAS  PubMed  Google Scholar 

  9. Bigger JT Jr, Steinman RC, Rolnitzky LM, Fleiss JL, Albrecht P, Cohen RJ (1996) Power law behavior of RR-interval variability in healthy middle-aged persons, patients with recent acute myocardial infarction, and patients with heart transplants. Circulation 93:2142–2151

    Article  PubMed  Google Scholar 

  10. Dekker JM, Crow RS, Folsom AR, Hannan PJ, Liao D, Swenne CA, Schouten EG (2000) Low heart rate variability in a 2-min rhythm strip predicts risk of coronary heart disease and mortality from several causes: the ARIC Study. Atherosclerosis risk in communities. Circulation 102:1239–1244

    Article  CAS  PubMed  Google Scholar 

  11. Farrell TG, Bashir Y, Cripps T, Malik M, Poloniecki J, Bennett ED, Ward DE, Camm AJ (1991) Risk stratification for arrhythmic events in postinfarction patients based on heart rate variability, ambulatory electrocardiographic variables and the signal-averaged electrocardiogram. J Am Coll Cardiol 18:687–697

    Article  CAS  PubMed  Google Scholar 

  12. Dinas PC, Koutedakis Y, Flouris AD (2013) Effects of active and passive tobacco cigarette smoking on heart rate variability. Int J Cardiol 163:109–115

    Article  PubMed  Google Scholar 

  13. Sondermeijer HP, van Marle AG, Kamen P, Krum H (2002) Acute effects of caffeine on heart rate variability. Am J Cardiol 90:906–907

    Article  CAS  PubMed  Google Scholar 

  14. Monda M, Viggiano A, Vicidomini C, Viggiano A, Iannaccone T, Tafuri D, De Luca B (2009) Espresso coffee increases parasympathetic activity in young, healthy people. Nutr Neurosci 12:43–48

    Article  CAS  PubMed  Google Scholar 

  15. Hibino G, Moritani T, Kawada T, Fushiki T (1997) Caffeine enhances modulation of parasympathetic nerve activity in humans: quantification using power spectral analysis. J Nutr 127:1422–1427

    CAS  PubMed  Google Scholar 

  16. Rauh R, Burkert M, Siepmann M, Mueck-Weymann M (2006) Acute effects of caffeine on heart rate variability in habitual caffeine consumers. Clin Physiol Funct Imaging 26:163–166

    Article  CAS  PubMed  Google Scholar 

  17. Yeragani VK, Krishnan S, Engels HJ, Gretebeck R (2005) Effects of caffeine on linear and nonlinear measures of heart rate variability before and after exercise. Depress Anxiety 21:130–134

    Article  CAS  PubMed  Google Scholar 

  18. Bunsawat K, White DW, Kappus RM, Baynard T (2015) Caffeine delays autonomic recovery following acute exercise. Eur J Prev Cardiol 22:1473–1479

    Article  PubMed  Google Scholar 

  19. Malliani A, Pagani M, Lombardi F, Cerutti S (1991) Cardiovascular neural regulation explored in the frequency domain. Circulation 84:482–492

    Article  CAS  PubMed  Google Scholar 

  20. Esco MR, Williford HN (2013) Relationship between post-exercise heart rate variability and skinfold thickness. Springerplus 2:389

    Article  PubMed  PubMed Central  Google Scholar 

  21. Suresh K (2011) An overview of randomization techniques: an unbiased assessment of outcome in clinical research. J Hum Reprod Sci 4:8–11

    Article  PubMed  PubMed Central  Google Scholar 

  22. Ryan EJ, Kim CH, Fickes EJ, Williamson M, Muller MD, Barkley JE, Gunstad J, Glickman EL (2013) Caffeine gum and cycling performance: a timing study. J Strength Cond Res 27:259–264

    Article  PubMed  Google Scholar 

  23. Gamelin FX, Berthoin S, Bosquet L (2006) Validity of the polar S810 heart rate monitor to measure R–R intervals at rest. Med Sci Sports Exerc 38:887–893

    Article  PubMed  Google Scholar 

  24. Richman JS, Moorman JR (2000) Physiological time-series analysis using approximate entropy and sample entropy. Am J Physiol Heart Circ Physiol 278:H2039–H2049

    CAS  PubMed  Google Scholar 

  25. Cornelis MC, El-Sohemy A, Campos H (2004) Genetic polymorphism of CYP1A2 increases the risk of myocardial infarction. J Med Genet 41:758–762

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Cohen J (1988) Statistical power for the behavioral sciences. Erlbaum, Hillsdale

    Google Scholar 

  27. Esco MR, Flatt AA, Williford HN (2015) Postexercise heart rate variability following treadmill and cycle exercise: a comparison study. Clin Physiol Funct Imaging. doi:10.1111/cpf.12308

    Google Scholar 

  28. Michael S, Jay O, Halaki M, Graham K, Davis GM (2016) Submaximal exercise intensity modulates acute post-exercise heart rate variability. Eur J Appl Physiol 116:697–706

    Article  PubMed  Google Scholar 

  29. Niskanen JP, Tarvainen MP, Ranta-Aho PO, Karjalainen PA (2004) Software for advanced HRV analysis. Comput Methods Programs Biomed 76:73–81

    Article  PubMed  Google Scholar 

  30. Karakaya O, Barutcu I, Kaya D, Esen AM, Saglam M, Melek M, Onrat E, Turkmen M, Esen OB, Kaymaz C (2007) Acute effect of cigarette smoking on heart rate variability. Angiology 58:620–624

    Article  PubMed  Google Scholar 

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Acknowledgments

This work was conducted at Chatham University in Pittsburgh, PA, USA. Support for this research was provided by the Research and Sabbatical Committee at Chatham University, and the Departments of Biology and Exercise Science at Chatham University.

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Authors and Affiliations

  1. Department of Biology, Chatham University, Pittsburgh, PA, USA

    R. M. Thomas, H. A. Algrain, A. Popojas, P. Carrigan & A. Abdulrahman

  2. Department of Exercise Science, Chatham University, Pittsburgh, PA, USA

    E. J. Ryan & A. E. Carrillo

  3. FAME Laboratory, Department of Exercise Science, University of Thessaly, Trikala, Greece

    A. E. Carrillo

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  1. R. M. Thomas
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  2. H. A. Algrain
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  3. E. J. Ryan
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  4. A. Popojas
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  5. P. Carrigan
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  6. A. Abdulrahman
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  7. A. E. Carrillo
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Correspondence to A. E. Carrillo.

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The authors declare no conflict of interest in relation to this study.

Research involving human participants and/or animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Thomas, R.M., Algrain, H.A., Ryan, E.J. et al. Influence of a CYP1A2 polymorphism on post-exercise heart rate variability in response to caffeine intake: a double-blind, placebo-controlled trial. Ir J Med Sci 186, 285–291 (2017). https://doi.org/10.1007/s11845-016-1478-7

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  • DOI: https://doi.org/10.1007/s11845-016-1478-7

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