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Irish Journal of Medical Science

, Volume 183, Issue 2, pp 259–264 | Cite as

Promoter hypermethylation-mediated down-regulation of RUNX3 gene in human brain tumors

  • C. B. AvciEmail author
  • Y. Dodurga
  • S. Y. Susluer
  • Z. O. D. Sıgva
  • M. Yucebas
  • H. O. Caglar
  • T. Akalin
  • T. Dalbasti
  • N. Oktar
  • C. Gunduz
Original Article

Abstract

Background

The Runx family proteins, including RUNX3, are tissue-restricted transcription factors and play role in neuronal development and tumorigenesis. RUNX3 has an important role in glioblastoma (GBM) tumorigenesis because of its promoter hypermethylation.

Aim

We aimed to evaluate the methylation-mediated expression regulation of RUNX3 gene in brain tumors.

Patients and methods

Cases of meningiomas WHO grade III (3), anaplastic astrocytomas (3), diffuse astrocytoma (3), and GBM (12) were recruited into this study. Real-time quantitative PCR was performed for analyses of DNA promoter methylation and analyses of methylation-mediated expression status of RUNX3 gene was performed by real-time quantitative RT-PCR.

Results

There was no significant difference between methylated and unmethylated quantitative ratio of RUNX3 gene promoter region and also no significant difference in relative ratio of RUNX3 gene expression in brain tumor groups. Methylated and unmethylated ratio in anaplastic astrocytoma, diffuse astrocytoma, GBM, meningioma (WHO grade III) and in all groups were; 1.44, 1.09, 1.51, 1.52 and 1.43, respectively. One allele was found methylated necessarily. No methylation was detected in one case of GBM group and one case of anaplastic astrocytoma group. There was no unmethylated promoter in one of the GBM cases. There were significant differences between relative ratio of RUNX3 gene expression and methylated/unmethylated ratio rates for all cases (p = 0.001) and GBM groups (p = 0.041).

Conclusion

This study overemphasized the RUNX3 gene importance in brain tumors, due to the existence of at least one methylated allele.

Keywords

Human brain tumors RUNX3 DNA methylation Gene expression 

Notes

Acknowledgments

This study is supported by Ege University Medical Faculty Research Project Subcommittee (Grant number: T-00033 APAK).

Conflict of interest

The author declare that there is no conflict of interest.

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Copyright information

© Royal Academy of Medicine in Ireland 2013

Authors and Affiliations

  • C. B. Avci
    • 1
    Email author
  • Y. Dodurga
    • 5
  • S. Y. Susluer
    • 1
  • Z. O. D. Sıgva
    • 1
  • M. Yucebas
    • 1
  • H. O. Caglar
    • 2
  • T. Akalin
    • 3
  • T. Dalbasti
    • 4
  • N. Oktar
    • 4
  • C. Gunduz
    • 1
  1. 1.Department of Medical BiologyEge University Medical FacultyBornovaTurkey
  2. 2.Department of Stem Cell, Health Science InstituteEge UniversityBornovaTurkey
  3. 3.Department of PathologyEge University Medical FacultyBornovaTurkey
  4. 4.Department of NeurosurgeryEge University Medical FacultyBornovaTurkey
  5. 5.Department of Medical Biology, School of MedicinePamukkale UniversityDenizliTurkey

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