Skip to main content

Advertisement

SpringerLink
Log in

Lactic acid bacterial extracts as anti-Helicobacter pylori: a molecular approach

  • Original Article
  • Published:
Irish Journal of Medical Science Aims and scope Submit manuscript

Abstract

Background

Helicobacter pylori (H. pylori) infection, the main cause of chronic gastritis, increases gastric cancer risk. Antibiotics-based H. pylori eradication treatment is 90 % effective. However, it is expensive and causes side effects and antibiotic resistance. Lactic acid bacteria (LAB) could present a low-cost, large-scale alternative solution to prevent or decrease H. pylori colonization.

Aim

This work aimed to study the inhibitory effects of LAB strains on the growth and pathogenic activity of H. pylori stains. To this end, we have selected the most virulent H. pylori strains (out of 20 mucosal antral biopsies) regarding cellular vacuolization and induction of apoptosis/necrosis.

Method

The selection of H. pylori pathogenic strains (clinically pre-isolated) were based on their impact of VacA activities on Hep-2 cell line, induction of apoptosis and necrosis in Caco-2 cell line. The Inhibitory effect of LAB strains on the invasion was carried out using the Caco-2 and Hela cell lines, where, they were co-cultured with the pathogenic H. pylori in the presence or absence of LAB extracts. The effect of LAB extracts on TNF-α secretion which induced by H. pylori-LPS was carried out by RT-qPCR.

Results

L. bulgaricus DSMZ 20080, L. acidophilus and L. plantarum (studied previously and reported as high antioxidant candidate strains) showed the highest anti-pylori activities with inhibition ranged from 51.46 to 88.19 %, they preventing the adhesion, invasion and DNA fragmentation of cell lines. In addition, they could reduce the TNF-α expression by 62.13 %.

Conclusion

LAB extracts could inhibit the bacterial adhesion and invasion, gastric inflammation and DNA fragmentation induced by Helicobacter pylori.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Stege PW, Davicino RC, Veja AE, Casali YA, Correa S, Micalizzi B (2006) Antimicrobial activity of aqueous extracts of Larrea divaricata Cav (jarilla) against Helicobacter pylori. Phytomedicine 13:724–727

    Article  PubMed  CAS  Google Scholar 

  2. Baldari CT, Lanzavecchia A, Telford JL (2005) Immune subversion by Helicobacter pylori. Trends Immunol 26:199–207

    Article  PubMed  CAS  Google Scholar 

  3. Noach LA, Bosma NB, Jansen J, Hoek FJ, van Deventer SJ, Tytgat GN (1994) Mucosal tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-8 production in patients with Helicobacter pylori infection. Scand J Gastroenterol 29:425–429

    Article  PubMed  CAS  Google Scholar 

  4. Peek RM, Crabtree JE (2006) Helicobacter infection and gastric neoplasia. J Pathol 208:233–248

    Article  PubMed  CAS  Google Scholar 

  5. Ogawa T, Asai Y, Sakai Y et al (2003) Endotoxic and immunobiological activities of a chemically synthesized lipid A of Helicobacter pylori strain 206-1. FEMS Immunol Med Microbiol 36:1–7

    Article  PubMed  CAS  Google Scholar 

  6. Castillo-Juárez I, González V, Jaime-Aguilar H et al (2009) Anti-Helicobacter pylori activity of plants used in Mexican traditional medicine for gastrointestinal disorders. J Ethnopharmacol 122(2):402–405

    Article  PubMed  Google Scholar 

  7. De Francesco V, Zullo A, Ierardi E et al (2009) Phenotypic and genotypic Helicobacter pylori clarithromycin resistance and therapeutic outcome: benefits and limits. J Antimicrob Chemother 65:327–332

    Article  PubMed  Google Scholar 

  8. Wolle K, Malfertheiner P (2007) Treatment of Helicobacter pylori. J Clin Gastroenterol 21(2):315–324

    CAS  Google Scholar 

  9. Jenks PJ, Edwards D (2002) Metronidazole resistance in Helicobacter pylori. Int J Antimicrob Agents 19:1–7

    Article  PubMed  CAS  Google Scholar 

  10. Weizman Z, Asli G, Alsheikh A (2005) Effect of a probiotic infant formula on infections in child care centers: comparison of two probiotic agents. Pediatrics 115:5–9

    PubMed  Google Scholar 

  11. Michetti P, Dorta G, Wiesel PH et al (1999) Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Digestion 60:203–209

    Article  PubMed  CAS  Google Scholar 

  12. Mukai T, Asasaka T, Sato E, Mori K, Matsumoto M, Ohori H (2002) Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri. FEMS Immunol Med Microbiol 32:105–110

    Article  PubMed  CAS  Google Scholar 

  13. Linsalata M, Russo F, Berloco P et al (2004) The influence of Lactobacillus brevis on ornithine decarboxylase activity and polyamine profiles in Helicobacter pylori-infected gastric mucosa. Helicobacter 9:165–172

    Article  PubMed  CAS  Google Scholar 

  14. El-Adawi H, Khalil AM, El-Sheekh MM, El-Deeb NM, Hussein MZ (2012) Cytotoxicity assay and antioxidant activities of the lactic acid bacterial strains. Afr J Microbiol Res 6(8):1700–1712

    Google Scholar 

  15. Borenfreund E, Puerner JA (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. J Toxicol Lett 24:119–124

    Article  CAS  Google Scholar 

  16. Backert S, Clyne M (2011) Pathogenesis of Helicobacter pylori infection. Helicobacter 16(S1):19–25

    Article  PubMed  CAS  Google Scholar 

  17. Kranzer K, Söllner L, Aigner M, Schneider-Brachert W (2005) Impact of Helicobacter pylori virulence factors and compounds on activation and maturation of human dendritic cells. Infect Immun 73(7):4180–4189

    Article  PubMed  CAS  Google Scholar 

  18. Rena J, Peter J, Nicola L (2004) Helicobacter pylori induces apoptosis of macrophages in association with alterations in the mitochondrial pathway. Infect Immun 72(5):2889–2898

    Article  Google Scholar 

  19. Knutton S, Lloyd DR, McNeish AS (1987) Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun 55(1):69–77

    PubMed  CAS  Google Scholar 

  20. Wilkinson SM, Uhl JR, Kline BC, Cockerill FR 3rd (1998) Assessment of invasion frequencies of cultured HEp-2 cells by clinical isolates of Helicobacter pylori using an acridine orange assay. J Clin Pathol 51:127–133

    Article  PubMed  CAS  Google Scholar 

  21. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284(5756):555–556

    Article  PubMed  CAS  Google Scholar 

  22. Uchida K, Mizushima S (1987) A simple methods for isolation of lipopolysaccharide from Pseudomonas aeruginosa and some other bacterial strains. Agric Biol Chem 51:3107–3114

    Article  CAS  Google Scholar 

  23. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-chloroform extraction. Anal Biochem 162:156–159

    Article  PubMed  CAS  Google Scholar 

  24. Stalke P, Abu Al-Soud W, Bielawski KP et al (2005) Detection of Helicobacter species in liver and stomach tissues of patients with chronic liver disease using polymerase chain reaction—denaturing gradient gel electrophoresis and immunohistochemistry. Scand J Gastroenterol 40:1032–1041

    Article  PubMed  CAS  Google Scholar 

  25. Calvet X, Navarro M, Gil M et al (1997) Seroprevalence and epidemiology of Helicobacter pylori infection in patients with cirrhosis. J Hepatol 26(6):1249–1254

    Article  PubMed  CAS  Google Scholar 

  26. Parsonnet J (1998) Helicobacter pylori: the size of the problem. Gut 43(Suppl 1):S6–S9

    Google Scholar 

  27. Tombola F, Carlesso C, Szabò I et al (1999) Helicobacter pylori vacuolating toxin forms anion-selective channels in planar lipid bilayers: possible implications for the mechanism of cellular vacuolation. Biophys J 76(3):1401–1409

    Google Scholar 

  28. Montecucco C, Papini E, de Bernard M et al (1999) Molecular and cellular activities of Helicobacter pylori pathogenic factors. FEBS Lett 452(1–2):16–21

    Google Scholar 

  29. de Bernard M, Burroni D, Papini E et al (1998) Identification of the Helicobacter pylori VacA toxin domain active in the cell cytosol. Infect Immun 66(12):6014–6016

    Google Scholar 

  30. Wu Y-Y, Tsai H-F, Lin W-C et al (2004) Helicobacter pylori enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in human gastric epithelial cells. World J Gastroenterol 10(16):2334–2339

    PubMed  CAS  Google Scholar 

  31. Bernet MF, Brassart D, Neeser JR, Servin AL (1994) Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut 35(4):483–489

    Article  PubMed  CAS  Google Scholar 

  32. Cats A, Kuipers EJ, Bosschaert MAR et al (2003) Effect of frequent consumption of a Lactobacillus casei-containing milk drink in Helicobacter pylori-colonized subjects. Aliment Pharmacol Ther 17:429–435

    Article  PubMed  CAS  Google Scholar 

  33. Vandenbergh PA (1993) Lactic acid bacteria, their metabolic products and interference with microbial growth. FEMS Microbiol Rev 12:221–238

    Article  CAS  Google Scholar 

  34. Sgouras D, Maragkoudakis P, Petraki K et al (2004) In vitro and in vivo inhibition of Helicobacter pylori by Lactobacillus casei strain Shirota. Appl Environ Microbiol 70(1):518–526

    Article  PubMed  CAS  Google Scholar 

  35. Guruge JL, Falk PG, Lorenz RG, Dans M et al (1998) Epithelial attachment alters the outcome of Helicobacter pylori infection. Proc Natl Acad Sci USA 95:3925–3930

    Article  PubMed  CAS  Google Scholar 

  36. Smoot DT (1997) How does Helicobacter pylori cause mucosal damage? Direct mechanisms. Gastroenterology 113(6 Suppl):S31–S34

    Article  PubMed  CAS  Google Scholar 

  37. Coconnier MH, Lievin V, Bernet-Camard MF, Hudault S, Servin AL (1997) Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB. Antimicrob Agents Chemother 41(5):1046–1052

    PubMed  CAS  Google Scholar 

  38. Nam HHM, Bae O, Lee Y (2002) Effect of Weissella confusa strain PL9001 on the adherence and growth of Helicobacter pylori. Appl Environ Microbiol 68:4642–4645

    Article  PubMed  CAS  Google Scholar 

  39. Jack RW, Tagg JR, Ray B (1995) Bacteriocins of gram-positive bacteria. Microbiol Mol Biol Rev 59(2):171–200

    CAS  Google Scholar 

  40. Ryan KA, Daly P, Li Y, Hooton C, O’Toole PW (2008) Strain-specific inhibition of Helicobacter pylori by Lactobacillus salivarius and other lactobacilli. J Antimicrob Chemother 61(4):831–834

    Article  PubMed  CAS  Google Scholar 

  41. Tegtmeyer N, Wessler S, Backert S (2011) Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis. FEBS J 278:1190–1202

    Google Scholar 

  42. Bhattacharyya A, Pathak S, Datta S, Chattopadhyay S, Basu J, Kundu M (2002) Mitogen-activated protein kinases and nuclear factor-kappaB regulate Helicobacter pylori-mediated interleukin-8 release from macrophages. Biochem J 368:121–129

    Article  PubMed  CAS  Google Scholar 

  43. Arakawa T, Higuchi K, Fujiwara Y et al (2000) Helicobacter pylori: criminal or innocent bystander? J Gastroenterol 35(Suppl 12):42–46

    PubMed  Google Scholar 

  44. Harris RA, Owens DK, Witherell H, Parsonnet J (1999) Helicobacter pylori and gastric cancer: what are the benefits of screening only for the CagA phenotype of H. pylori? Helicobacter 4:69–76

    Article  PubMed  CAS  Google Scholar 

  45. Gill HS, Rutherfurd KJ, Cross ML, Gopal PK (2001) Enhancement of immunity in the elderly by dietary supplementation with the probiotic B lactis HN019. Am J Clin Nutr 74:833–839

    PubMed  CAS  Google Scholar 

  46. Haller D, Bode C, Hammes WP, Pfeifer AM, Schiffrin EJ, Blum S (2000) Non-pathogenic bacteria elicit a differential cytokine response by intestinal epithelial cell/leucocyte co-cultures. Gut 47:79–87

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Hanan Sloiman and Dr. Asem Elfert (Department of Tropical Medicine & Infectious Diseases, Faculty of Medicine-Tanta University) for kindly providing the biopsy specimens.

Author information

Authors and Affiliations

  1. Department of Medical Biotechnology, Genetic Engineering and Biotech Institute, Mubarak City for Scientific Research, Universities and Research District Sector, New Borg El-Arab, P.O.BOX 29134, Alexandria, Egypt

    H. El-Adawi & N. El-Deeb

  2. Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt

    M. El-Sheekh & M. Khalil

  3. Faculty of Medicine, Tanta University, Tanta, Egypt

    M. Hussein

Authors
  1. H. El-Adawi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. El-Sheekh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Khalil
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. El-Deeb
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Hussein
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. El-Adawi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

El-Adawi, H., El-Sheekh, M., Khalil, M. et al. Lactic acid bacterial extracts as anti-Helicobacter pylori: a molecular approach. Ir J Med Sci 182, 439–452 (2013). https://doi.org/10.1007/s11845-013-0909-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11845-013-0909-y

Keywords

Search

Navigation