First Irish delivery following sequential, two-stage embryo and blastocyst transfer
The timing of embryo transfer (ET) after in vitro fertilisation (IVF) remains controversial, and there are no reliable guidelines available to prospectively identify which patients would benefit from either day-3 or blastocyst transfer. While blastocyst transfer is generally favoured over day-3 transfers, very few IVF patients get both in the same treatment cycle.
We report on a 35.5-year-old female with tubal factor infertility who underwent IVF, which included transfer of a fresh day-3 embryo and a thawed blastocyst frozen at day 6. Transfer occurred on two separate days (days 3 and 6) in a two-stage/dual catheter fashion and resulted in a healthy term singleton livebirth.
While combined day-3 and day-5 ET has been available elsewhere for several years, this is the first description of its successful application in Ireland and confirms the effectiveness of coordinated two-stage transfer in a single IVF treatment cycle.
KeywordsEmbryo transfer Sequential Two stage Blastocyst
Embryo transfer (ET) is typically a single-step process where all embryos destined for the uterus are placed by one catheter. The timing of ET has long been controversial, but as the human embryo normally arrives inside the uterine compartment from the oviduct at the blastocyst stage, many authorities have advocated this as a more natural and physiologically preferred approach, superior to day 3 ET. Moreover, when transferred at day 2 or 3, embryo development is directed by maternal transcripts and stored mRNA exclusively from the oocyte only, because the embryonic genome remains latent at this time. Although blastocyst transfer has distinct advantages over earlier transfer protocols in in vitro fertilisation (IVF), intentionally culturing all embryos to blastocyst stage brings the risk of cancelling the transfer should no embryos survive in culture. To avoid this unfortunate outcome, a strategy of consecutive (two stage) ET has been described [1, 2] where the patient first has a day-3 transfer followed by a second (blastocyst) transfer in the same cycle—usually 3 days later. Although deployed elsewhere for several years, this two-stage approach to ET for IVF patients has not been reported in Ireland until now.
A 35.5-year-old non-smoking Caucasian G2 P1011 attended for reproductive endocrinology consultation with a history of bilateral tubal disease and unilateral oophorectomy. Two years previously, the patient underwent IVF with a two-ET (at day 3) which resulted in a healthy singleton term livebirth; that treatment cycle also produced two embryos which were cryopreserved at day 3 but did not survive thaw. She returned for a second fresh IVF cycle, but follicular recruitment was poor and only one blastocyst was available for transfer. Unfortunately, her transfer was cancelled secondary to an intrauterine fluid collection identified on transvaginal ultrasound. The single blastocyst was frozen at day 6 with spontaneous resolution of the endometrial fluid the following week.
Embryo transfer is the final and perhaps most critical step in determining IVF outcome. Indeed, it has been estimated that poor ET technique may account for many IVF failures—perhaps up to 30% of unsuccessful cycles . It is generally recognised that the skill of the clinician performing ET is more important than catheter type or day of transfer, although most ETs in IVF are done as one procedure where the uterine cavity receives all cultured embryos from a single catheter at the same time. However, recent work has suggested that a two-stage ET specifically for patients with prior IVF failure can yield significantly higher pregnancy rates compared with those having traditional one-step ET [4, 5]. Which ET procedure resulted in the ongoing singleton pregnancy cannot be known with certainty in our patient, but since allied research has suggested that performing an endometrial biopsy prior to actual transfer induces a favourable endometrial response , we speculate that our patient’s viable pregnancy may be a continuation of the blastocyst transfer.
In this report, we confirm the effectiveness of coordinated two-stage transfer in a single cycle. Considerable multi-centre data have shown that offering extended in vitro culture and blastocyst transfer, whenever possible, can provide significantly improved pregnancy rates compared with traditional day-3 transfer [7, 8]. Day-3 transfer can still be appropriate for some patients, but there are no reliable guidelines available to prospectively identify which patients would benefit from each treatment approach . Anticipating blastocyst transfer for every IVF patient is inappropriate and risks cycle cancellation due to culture failure, although we are unaware of any published data identifying unsuccessful blastocyst culture as a specific stressor causing patients to abandon IVF. Nevertheless, patient education and counselling are a crucial component of the pre-treatment programme for IVF. Although blastocyst transfer is generally favoured at our institution, day 3 transfers are still performed when clinically indicated. Very few IVF patients get both in the same treatment cycle, however. The current case illustrates a plan tailored to meet the disparate physiological needs of very different embryos, and presents the first published report of a delivery in Ireland after sequential ETs. Additional studies are underway to evaluate this treatment approach for other couples referred for prior IVF failure.
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