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Multiscale Effects of Collagen Damage in Cortical Bone and Dentin

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Abstract

The denaturation of collagen at the molecular level in bone and dentin can impact their structure and properties, leading to increased brittleness in pathological diseases such as osteogenesis imperfecta, dentinogenesis imperfecta, diabetes, and cancer. This study investigates the relationship between collagen denaturation and the macroscale resistance of bone and dentin. Through heat treatment at \(160{^{\circ }}\hbox {C}\) on bovine bone and human dentin, the effects of collagen denaturation on macroscale flexural strength, scanning electron microscopy, and transmission electron microscopy imaging of micro- and nanostructure were studied. The results show that collagen denaturation decreases the resistance of bone and dentin to fracture, even though collagen denaturation did not impact the mineral organization around and inside collagen fibrils. This is attributable to (1) a reduction in bone and dentin ability to deform (e.g., 40–75% decrease in strain to failure) and to resist fracture (e.g., 83–95% decrease in work to fracture) properties and (2) to a smoother crack path with less crack deflection around microstructural features. Reduction in deformation and toughness not only removed plastic deformation but also drastically decreased elastic deformation and elastic work to fracture in all tissues. However, the elastic modulus was only affected in radial-oriented bone samples where collagen fibrils are oriented perpendicularly to crack opening forces. This study highlights the crucial role of collagen molecule integrity and orientation in bone/dentin deformability and resistance.

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Acknowledgements

This work has been supported as part of France 2030 program ANR-11-IDEX-0003.

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Authors and Affiliations

  1. LMPS, Université Paris-Saclay, CentraleSupélec, ENS Paris-Saclay, CNRS, LMPS - Laboratoire de Mécanique Paris-Saclay, 3 Rue Joliot Curie, 91190, Gif-sur-Yvette, France

    Qian Wu, Shangaya Touraivane, Thomas Reiss, Maxime Vallet, Nicolas Roubier & Elsa Vennat

  2. Université Paris-Saclay, CentraleSupélec, ENS Paris-Saclay, CNRS, Laboratoire SPMS, 3 Rue Joliot Curie, 91190, Gif-sur-Yvette, France

    Maxime Vallet

  3. Department of Mechanical Engineering, University of Utah, Salt Lake City, UT, 84112, USA

    Claire Acevedo

  4. Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, 84112, USA

    Claire Acevedo

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  1. Qian Wu
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CA and EV designed research; QW, TR and MV performed research; QW, ST analyzed data; CA, EV, QW, ST wrote the manuscript. All authors have given approval for the final version of the manuscript.

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Wu, Q., Touraivane, S., Reiss, T. et al. Multiscale Effects of Collagen Damage in Cortical Bone and Dentin. JOM 75, 2102–2113 (2023). https://doi.org/10.1007/s11837-023-05852-0

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