Skip to main content
SpringerLink
Log in

Fabrication and Characterization of Sr-doped Hydroxyapatite Porous Scaffold

  • Multiscale Experiments and Modeling in Biomaterials and Biological Materials
  • Published:
JOM Aims and scope Submit manuscript

Abstract

Strontium (Sr) is a trace metal ion that exists in natural bones. It not only regulates metabolism but also promotes osteoblast differentiation and the formation of new bones. In this study, a new method was developed to prepare Sr-doped hydroxyapatite (HA) three-dimensional (3D) porous scaffolds. A preliminary scaffold was obtained by an extrusion 3D printing method using HA–sodium alginate composite slurry as printing ink. Then, the obtained scaffolds were immersed in a strontium chloride (SrCl2·6H2O) solution of different concentrations (1 wt.%, 5 wt.%, 10 wt.%, and 15 wt.%) for 24 h. Finally, the scaffolds were dried and then sintered at 1200°C to obtain the final scaffolds. The Sr-doped scaffolds were characterized by scanning electron microscopy, x-ray diffraction, Fourier-transform infrared spectroscopy, mechanical testing, and cell culture testing. The results show that the Sr-doped HA scaffold has better compactness and compressive strength than the HA-0Sr scaffold, and promotes cell proliferation and osteoblast differentiation. This indicates that the alternative method is suitable for preparing ion-doping ceramic scaffolds for bone tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. B. Dhandayuthapani, Y. Yoshida, T. Maekawa, D. S. Kumar and S. Wang, Int. J. Polym. Sci. 290602 (2011).

  2. P.N. Kumta, C. Sfeir, D. Lee, D. Olton, and D. Choi, Acta Biomater. 1(1), 65. (2005).

    Article  Google Scholar 

  3. I. Ullah, M.A. Siddiqui, S.K. Kolawole, H. Liu, J. Zhang, L. Ren, and K. Yang, Ceram. Int. 46(10, Part A), 14448. (2020).

    Article  Google Scholar 

  4. G. Tripathi, and B. Basu, Ceram. Int. 38(1), 341. (2012).

    Article  Google Scholar 

  5. B.K. Culpepper, M.C. Phipps, P.P. Bonvallet, and S.L. Bellis, Biomaterials 31(36), 9586. (2010).

    Article  Google Scholar 

  6. A. Szcześ, L. Hołysz, and E. Chibowski, Adv. Colloid Interface Sci. 249, 321. (2017).

    Article  Google Scholar 

  7. N. Lowry, Y. Han, B.J. Meenan, and A.R. Boyd, Ceram. Int. 43(15), 12070. (2017).

    Article  Google Scholar 

  8. T.F. Sun, K. Zhou, M. Liu, X.D. Guo, Y.Z. Qu, W. Cui, Z.W. Shao, X.L. Zhang, and S.Y. Xu, J. Tissue Eng. Regener. Med. 12(4), 846. (2018).

    Article  Google Scholar 

  9. X. Zhao, Y. Han, J. Li, B. Cai, H. Gao, W. Feng, S. Li, J. Liu, and D. Li, Mater. Sci. Eng. C 78, 658. (2017).

    Article  Google Scholar 

  10. S. Bose, and S. Tarafder, Acta Biomater. 8(4), 1401. (2012).

    Article  Google Scholar 

  11. B.Y. Ozturk, I. Inci, S. Egri, A.M. Ozturk, H. Yetkin, G. Goktas, C. Elmas, E. Piskin, and D. Erdogan, Eur. J. Orthopaed. Surg. Traumatol. 23(7), 767. (2013).

    Article  Google Scholar 

  12. W. Zhang, Y. Tian, H. He, R. Chen, Y. Ma, H. Guo, Y. Yuan, and C. Liu, Acta Biomater. 33, 290. (2016).

    Article  Google Scholar 

  13. L. Ludmila, R. Anne-Laure, H.W. Beat, G. Robert, and J. Olivier, J. Control Release 147(1), 38. (2010).

    Article  Google Scholar 

  14. T. Tite, A.-C. Popa, L.M. Balescu, I.M. Bogdan, I. Pasuk, J.M.F. Ferreira, and G.E. Stan, Materials 11, 11. (2018).

    Article  Google Scholar 

  15. I. Ullah, W. Li, S. Lei, Y. Zhang, W. Zhang, U. Farooq, S. Ullah, M.W. Ullah, and X. Zhang, Ceram. Int. 44(17), 21338. (2018).

    Article  Google Scholar 

  16. O. Vincent, O.Z. Andersen, M. Sillassen, K.P. Almtoft, I.H. Andersen, F. Kloss, and M. Foss, Int. J. Nanomed. 13, 2189. (2018).

    Article  Google Scholar 

  17. Y. Okuzu, S. Fujibayashi, S. Yamaguchi, K. Yamamoto, T. Shimizu, T. Sono, K. Goto, B. Otsuki, T. Matsushita, T. Kokubo, and S. Matsuda, Acta Biomater. 63, 383–392. (2017).

    Article  Google Scholar 

  18. M. Avci, B. Yilmaz, A. Tezcaner, and Z. Evis, Ceram. Int. 43(12), 9431. (2017).

    Article  Google Scholar 

  19. V. Aina, L. Bergandi, G. Lusvardi, G. Malavasi, F.E. Imrie, I.R. Gibson, G. Cerrato, and D. Ghigo, Mater. Sci. Eng. C 33(3), 1132. (2013).

    Article  Google Scholar 

  20. S. Taherkhani, and F. Moztarzadeh, J. Sol-Gel Sci. Technol. 78(3), 539. (2016).

    Article  Google Scholar 

  21. Y.J. No, S. Roohaniesfahani, Z. Lu, J. Shi, and H. Zreiqat, Biomed. Mater. 12, 3. (2017).

    Article  Google Scholar 

  22. Y. Deng, M. Liu, X. Chen, M. Wang, X. Li, Y. Xiao, and X. Zhang, J. Mater. Chem. B 6(41), 6572. (2018).

    Article  Google Scholar 

  23. S. Zhao, J. Zhang, M. Zhu, Y. Zhang, Z. Liu, C. Tao, Y. Zhu, and C. Zhang, Acta Biomater. 12, 270. (2015).

    Article  Google Scholar 

  24. I. Denry, and L.T. Kuhn, Dent. Mater. 32(1), 43. (2016).

    Article  Google Scholar 

  25. K. Zhou, X. Zhang, Z. Chen, L. Shi, and W. Li, Ceram. Int. 41(10, Part B), 14029. (2015).

    Article  Google Scholar 

  26. B.F. Kieback, G. Leitner, and K. Pischang, J. Therm. Anal. 33(2), 559. (1988).

    Article  Google Scholar 

  27. C.F. Marques, S. Olhero, J.C.C. Abrantes, A. Marote, S. Ferreira, S.I. Vieira, and J.M.F. Ferreira, Ceram. Int. 43(17), 15719. (2017).

    Article  Google Scholar 

  28. C.J. Zhang, N.N. Zhang, Z. Wang, and P. Zhu, Dye Finish. 37(08), 1. (2011).

    Google Scholar 

  29. J. Zhang, H. Tanaka, F. Ye, D. Jiang, and M. Iwasa, Mater. Chem. Phys. 101(1), 69. (2007).

    Article  Google Scholar 

  30. N.C. Reger, B. Kundu, B.V.K. Biswanath, and A.K. Bhargava, Int. J. Appl. Ceram. Technol. 16, 2. (2019).

    Article  Google Scholar 

  31. H. Zhu, D. Guo, L. Sun, H. Li, D.A.H. Hanaor, F. Schmidt, and K. Xu, J. Eur. Ceram. Soc. 38(16), 5554. (2018).

    Article  Google Scholar 

  32. C.M. Mardziah, I. Sopyan, and S. Ramesh, Trends Biomater. Artif. Organs 2(23), 105. (2009).

    Google Scholar 

  33. Q. Wu, X. Zhang, B. Wu, and W. Huang, Ceram. Int. 39(3), 2389. (2013).

    Article  Google Scholar 

  34. M. Mazaheri, M. Haghighatzadeh, A.M. Zahedi, and S.K. Sadrnezhaad, J. Alloys Compd. 471(1), 180. (2009).

    Article  Google Scholar 

  35. Z. Chen, X. Zhang, Y. Yang, K. Zhou, N. Wragg, Y. Liu, M. Lewis, and C. Liu, Ceram. Int. 43(1, Part A), 336. (2017).

    Article  Google Scholar 

  36. K. Zhou, C. Dong, X. Zhang, L. Shi, Z. Chen, Y. Xu, and H. Cai, Ceram. Int. 41, 1. (2015).

    Article  Google Scholar 

  37. M.P. Moreira, V.T. da Silva Aragão, G.D. de Almeida Soares, and E.A. dos Santos, Key Eng. Mater. 1463, 20. (2012).

    Google Scholar 

  38. T. Joice, D.E. Rodrigues, E. Jean-Guillaume, E. Donald, G. Gabriela, and R.A. Malta, Phys. Chem. Chem. Phys. 11(3), 568. (2009).

    Article  Google Scholar 

  39. G. Renaudin, P. Laquerrière, Y. Filinchuk, E. Jallot, and J.M. Nedelec, J. Mater. Chem. 18(30), 3593. (2008).

    Article  Google Scholar 

  40. J. He, K. Zhang, S. Wu, X. Cai, K. Chen, Y. Li, B. Sun, Y. Jia, F. Meng, Z. Jin, L. Kong, and J. Liu, J. Hazard. Mater. 303, 119. (2016).

    Article  Google Scholar 

  41. N. Nassif, F. Martineau, O. Syzgantseva, F. Gobeaux, M. Willinger, T. Coradin, S. Cassaignon, T. Azaïs, and M.M. Giraud-Guille, Chem. Mater. 22(12), 3653. (2010).

    Article  Google Scholar 

  42. D.M. Andrade Neto, E.V. Carvalho, E.A. Rodrigues, V.P. Feitosa, S. Sauro, G. Mele, L. Carbone, S.E. Mazzetto, L.K. Rodrigues, and P.B.A. Fechine, Dent. Mater. 32(6), 784. (2016).

    Article  Google Scholar 

  43. Y. Li, D. Li, and Z. Xu, J. Mater. Sci. 44(5), 1258. (2009).

    Article  Google Scholar 

  44. M. Riaz, R. Zia, A. Ijaz, T. Hussain, M. Mohsin, and A. Malik, Mater. Sci. Eng. C 90, 308. (2018).

    Article  Google Scholar 

  45. D. Núñez, E. Elgueta, K. Varaprasad, and P. Oyarzún, Mater Lett. 230, 64. (2018).

    Article  Google Scholar 

  46. A. Oryan, M. Baghaban Eslaminejad, A. Kamali, S. Hosseini, F.A. Sayahpour, and H. Baharvand, J. Biomed. Mater. Res. B 107(1), 50. (2018).

    Article  Google Scholar 

  47. Q. Fu, M.N. Rahaman, H. Fu, and X. Liu, J. Biomed. Mater. Res. A 95A(1), 172. (2010).

    Article  Google Scholar 

  48. J.M. Cordell, M.L. Vogl, and A.J. Wagoner Johnson, J. Mech. Behav. Biomed. Mater. 2(5), 560. (2009).

    Article  Google Scholar 

  49. M. Wu, P. Wu, L. Xiao, Y. Zhao, F. Yan, X. Liu, Y. Xie, C. Zhang, Y. Chen, and L. Cai, Int. J. Biol. Macromol. 162, 1627. (2020).

    Article  Google Scholar 

  50. X.J. Wang, Y.C. Li, J.G. Lin, Y. Yamada, P.D. Hodgson, and C.E. Wen, Acta Biomater. 4(5), 1530. (2008).

    Article  Google Scholar 

  51. G. Kirmizidis, and M.A. Birch, Tissue Eng. A 15(6), 1427. (2009).

    Article  Google Scholar 

  52. R. Dimitriou, E. Jones, D. McGonagle, and P.V. Giannoudis, BMC Med. 9, 66. (2011).

    Article  Google Scholar 

  53. Z. He, Q. Zhai, M. Hu, C. Cao, J. Wang, H. Yang, and B. Li, J. Orthop. Transl. 3(1), 1. (2015).

    Google Scholar 

  54. X. Ma, Z. He, F. Han, Z. Zhong, L. Chen, and B. Li, Colloids Surf. B 143, 81. (2016).

    Article  Google Scholar 

  55. I. Cacciotti, and I.V. Antoniac, Handbook of Bioceramics and Biocomposites (Springer, Cham, 2014), pp 1–68.

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (No. 32001017, 31960207, 52061031). This work was also supported by Nanchang Municipal Key Laboratory of 3D Bioprinting Technology and Equipment (No. 2019NCZDSY001).

Author information

Authors and Affiliations

  1. School of Mechatronics Engineering, Nanchang University, Nanchang, 330031, People’s Republic of China

    Ke Li, Shuiyuan Li, Fanrong Ai, Jinchao Yan & Kui Zhou

  2. Nanchang Municipal Key Laboratory of 3D Bioprinting Technology and Equipment, Nanchang University, Nanchang, 330031, People’s Republic of China

    Fanrong Ai & Kui Zhou

Authors
  1. Ke Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuiyuan Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fanrong Ai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jinchao Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kui Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kui Zhou.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (PDF 739 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, K., Li, S., Ai, F. et al. Fabrication and Characterization of Sr-doped Hydroxyapatite Porous Scaffold. JOM 73, 1745–1753 (2021). https://doi.org/10.1007/s11837-021-04684-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11837-021-04684-0

Search

Navigation