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Intérêt de la spectrométrie de masse de type MALDI-TOF pour l’identification des champignons filamenteux

Interest of MALDI-TOF mass spectrometry for the identification of filamentous fungi

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Bio tribune magazine

Résumé

L’identification des champignons filamenteux, moisissures et dermatophytes, repose actuellement sur l’étude morphologique macroscopique et microscopique des colonies et, par conséquence, sur l’expérience du mycologue. Cette approche longue et fastidieuse, souvent imprécise, est incompatible avec les exigences actuelles de diagnostic des infections fongiques. L’identification des champignons filamenteux par MALDI-TOF est un champ d’innovation en pleine expansion. Bien que très peu d’études soient encore disponibles, les premiers travaux ont montré que la spectrométrie de masse MALDI-TOF permet d’obtenir une identification précise des espèces de champignons filamenteux, y compris au sein des complexes d’espèces. La spectrométrie de masse MALDI-TOF représente ainsi le premier outil permettant une identification rapide, précise et standardisée des champignons filamenteux. Les bases de données actuellement disponibles doivent être désormais évaluées dans les différents laboratoires de mycologie cliniques et complétées afin d’élargir le spectre des espèces identifiées.

Abstract

Identification of filamentous fungi, molds and dermatophytes, is currently based on the morphological study of colonies and therefore the experience of the mycologist. These techniques are not sufficiently precise to distinguish between different species within the same section. Furthermore, identification can be delayed for several weeks due to subcultures on specific media. MALDI-TOF MS allows correct identification of filamentous fungi until the species level in more than 95% of cases in most studies. MALDI-TOF MS is a fast and precise identification technique for filamentous fungi; however most of the different databases need to be further evaluated in routine and completed to broaden the spectrum of species identified.

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Authors and Affiliations

  1. Service de microbiologie, hôpital Necker Enfants Malades, APHP, 149 rue de Sèvres, 75015, Paris, France

    M. -É. Bougnoux, N. Hassouni, J. -L. Beretti & B. Dauphin

  2. Service de parasitologie-mycologie, hôpital Saint Louis, APHP, 75010, Paris, France

    C. Lacroix

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  1. M. -É. Bougnoux
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  2. C. Lacroix
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  3. N. Hassouni
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  4. J. -L. Beretti
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  5. B. Dauphin
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Correspondence to M. -É. Bougnoux.

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Bougnoux, M.É., Lacroix, C., Hassouni, N. et al. Intérêt de la spectrométrie de masse de type MALDI-TOF pour l’identification des champignons filamenteux. Bio trib. mag. 40, 45–49 (2011). https://doi.org/10.1007/s11834-011-0064-6

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  • DOI: https://doi.org/10.1007/s11834-011-0064-6

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