Bio tribune magazine

, Volume 40, Issue 1, pp 19–30 | Cite as

Spectrométrie de masse au laboratoire d’analyses: validation de méthode. Bilan de 15 mois d’utilisation

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Résumé

Cette étude rapporte l’expérience de l’utilisation en routine pendant 15 mois d’un spectromètre de masse (SM) de type MALDI-TOF (MALDI Biotyper-Microflex®, Bruker Daltonics) sur un plateau technique de bactériologie traitant en moyenne 250 échantillons par jour. Les résultats de validation de méthode (répétabilité et reproductibilité) à partir de 40 souches de référence sont présentés. Sur les 22 bactéries à Gram négatif, deux souches ont été mal identifiées et deux autres n’ont permis qu’une identification du genre. Pour les bactéries à Gram positif, les 18 souches testées ont toutes été correctement identifiées.

L’intérêt de la SM pour le laboratoire est décrit et illustré à l’aide d’exemples cliniques. Une étude économique est également présentée.

Mots clés

Identification microbienne MALDI-TOF validation de méthode 

Mass spectrometry in a medical laboratory: Outcome of 15 months of use

Abstract

This study reports the experiment of the routine use for 15 months of a mass spectrometer (MS) type MALDI-TOF (MALDI Biotyper-Microflex®, Bruker Daltonics) in a medical microbiology centre of a private laboratory, analyzing an average of 250 bacteriology samples per day. The results of the method’s validation tests (repeatability and reproducibility), from 40 reference strains, are shown. From 22 Gram-negative bacteria, two strains have been misidentified and two others only on genus. For the Gram-positive bacteria, the 18 tested strains have all been correctly identified to the species with first scores on average all exceeding 1.90.

Finally, the benefit of the introduction of mass spectrometry is described with several examples of clinical results as well as an economic approach.

Keywords

Microbial identification MALDI-TOF method’s validation 

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Copyright information

© Springer Verlag France 2011

Authors and Affiliations

  1. 1.Laboratoire BIOXAReimsFrance

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